纖纖四糖 的英文怎麼說
中文拼音 [xiānxiānsìtáng]
纖纖四糖
英文
cellotetrose-
On the lower shelf five vertical breakfast plates, six horizontal breakfast saucers on which rested inverted breakfast cups, a moustachecup, uninverted, and saucer of crown derby, four white goldrimmed eggcups, and open shammy purse displaying coins, mostly copper, and a phial of aromatic violet comfits. on the middle shelf a chipped eggcup containing pepper, a drum of table salt, four conglomerated black olives in oleaginous paper, an empty pot of plumtree s potted meat, an oval wicker basket bedded with fibre and containing one jersey pear, a halfempty bottle of william gilbey and co s white invalid port, half disrobed of its swathe of coralpink tissue paper, a packet of epps s soluble cocoa, five ounces of anne lynch s choice tea at 2 - per lb. in a crinkled leadpaper bag, a cylindrical canister containing the best crystallised lump sugar, two onions, one the larger, spanish, entire, the other, smaller, irish, bisected with augmented surface and more redolent, a jar of irish model dairy s cream, a jug of brown crockery containing a noggin and a quarter of soured adulterated milk, converted by heat into water, acidulous serum and semisolidified curds, which added to the quantity subtracted for mr bloom s and mrs fleming s breakfasts made one imperial pint, the total quantity originally delivered, two cloves, a halfpenny and a small dish containing a slice of fresh ribsteak
中層放著一隻盛了胡椒粉的有缺口的蛋杯，飯桌上還擺著那種鼓狀食鹽瓶，用油紙包著的四顆粘成一團的黑色橄欖，一聽李樹商標肉罐頭36的空罐兒，墊著纖絲的橢圓形柳條筐里是一隻澤西37梨，喝剩下的半瓶威廉吉爾比公司38釀造的藥用白葡萄酒裹在瓶子上的粉珊瑚色薄繪紙已剝掉了一半，一包埃普斯公司製造的速溶可可一隻縐錫紙袋裡裝著安妮林奇公司39出品的五英兩特級茶葉，每磅二先令一隻圓筒形罐子，盛著優質結晶角沙糖兩顆蔥頭，較大的那顆西班牙種的是完整的，較小的那顆愛爾蘭種的已經切成兩瓣兒，面積擴大了，氣味也更沖鼻了一罐愛爾蘭模範奶場的乳酪，一隻褐色陶罐，盛著四分之一品脫零四分之一兌了水並變酸了的牛奶由於炎熱，它已化為水酸性乳漿與半固體凝乳，再加上布盧姆先生和弗萊明大媽40作為早餐消費掉的部分，就足夠一英品脫了，相當于原先送來的總量兩朵丁香花蕾，一枚半便士硬幣和盛有一片新鮮排骨肉的一個小碟子。This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan
在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。The greater amount of dietary fibre, as much as four times than found in refined grains, is likely the most important benefit, as it has been shown to reduce the incidence of some forms of cancer, digestive system diseases, coronary heart disease, diabetes, and obesity
粗糧帶來的最重要的好處是其富含的四倍于細糧的纖維素，據研究顯示可以降低某些癌癥、消化系統疾病、冠心病、糖尿病以及肥胖癥的發病率。Methods the 54th generation of transformed human embryonic tendon cells and artificial composite materials of carbon fibers ( cf ) and polyglycolic ( pga ) were co - cultured in vitro to construct tet. lt was frozen in liquid nitrogen with four kinds of cpa for 2 months. post - thawed quickly and transplanted into hind limbs of nude mice, and repaired the defects of achilles tendon. after 2, 4, 6, 8, 12 weeks, the morphological, histological, ultrastructure, short tandem repeat loci and immunohistochemistry examination were detected, and biomechanical strength of tet were examined. result tendon cell survived and could secret type i collagen after 12 weeks to transplanted into nude mice. in the group of dmso + raffmose + kh2o4, vacuole in mitochondrion degraded i tendon cell ranged in order, abundant collagen fibers were found and linked each other and the biomechanical strength was increased as time elapsed. c onclusion dmso + raffmose + kh2o4 could protect tet in deep low temperature
組織工程肌腱制備完成後在四種抗凍劑保護下液氮凍存2月；快速復溫后植入裸鼠以修復跟腱缺損， 2 、 4 、 6 、 8 、 12周后取出，觀察形態學、組織學、電鏡和免疫組織化學變化，短串聯重復位點檢測和生物力學變化。結果實驗組組織工程肌腱體內植入12周后仍有肌腱細胞存活並分泌型膠原；隨著時間延長， 10二甲基亞碸（ dmso ） +棉子糖（ 30mmol l ） + kh _ 2po _ 4 （ 25mmol l ）組線粒體空泡減少，肌腱細胞排列整齊，膠原纖維增粗並連接，抗拉強度增高。分享友人