缺失誘變 的英文怎麼說
中文拼音 [quēshīyòubiàn]
缺失誘變
英文
deletion mutagenesis-
Two magnetosome deletion mutants were constructed by conjugative transposon mutagensis and the application of this genetic system. the two magnetosome deletion mutants were named as nm4 and nm21 respectively
並利用此體系，通過接合轉座誘變技術，獲得了2個磁小體缺失突變株： nm4 、 nm21 。( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1. 2 gene promoter. these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants. the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene, and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling
（ 2 ）對該啟動子的5 』端進行不同長度的缺失突變，突變的啟動子與gus構建的融合基因在煙草中受meja誘導的瞬時表達結果表明，該啟動子中- 300 - 243bp區域（有一個gccbox和一個gbox - likesequence ）是其應答meja處理所必須的區域，並將- 300bp作為該啟動子應答ja信號的最小區域的邊界位置。( 3 ) on the basis of the deletion analysis, three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element. transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation. loss - of - function experiment, using transient expression analysis of gus reporter genes, confirmed that gcc box act as an essential element to respond ja signaling in pdf1. 2 promoter
（ 3 ）在缺失突變的基礎上，通過對gccbox及其相鄰的上下游六個堿基進行取代突變，將突變啟動子與gus構建融合基因，在煙草中受heja誘導的瞬時表達結果表明， h1和m3的突變對該啟動子應答ja信號的影響很小，而m2 （ gccbox的突變）則幾乎使該啟動子應答ja信號的功能完全喪失，所以gccbox是該啟動子中應答ja信號的必需元件。In this paper, an important cis - acting element within the promoter of pdf7. 2, which is activated by jas, was analyzed, and the interaction of this element with relevant factors ( jerfs ) was also studied. the results were as fellows : la ja responsive cis - acting element within the promoter of pdf 1. 2 was investigated via comprehensive mutant analysis. ( 1 ) a strategy based on cdna sequence was used to amplify the promoter ofpdf1. 2 gene with arabidopsis gdna as template
本文對受jas誘導的pdf1 . 2啟動子的順式作用元件及其與jerfs的相互作用進行了系統研究，取得如下結果： 1 、通過對pdf1 . 2啟動子的缺失突變分析和取代突變分析，對該啟動子應答jas信號的順式作用元件進行了較詳細的探討。Two pta - mutants have been selected by using suicide substrate after the mini - tn5 transposon insertion mutagenesis of klebsiella pneumoniae m5al. when used in microaerobic fermentation, the amount of acetate produced by the mutants reduced to less than 50 % of the parent strain, and the yields improved whereas the 1, 3 - propanediol titers and productivities decreased
以klebsiellapneumoniaem5al為出發菌株，用mini - tn5隨機轉座誘變結合自殺性底物篩選的方法得到了兩株產乙酸途徑pta基因缺失突變株xl - 6和xl - 11 ，應用到微氧法發酵中，突變株的乙酸產量為親株的50以下，甘油轉化率有所提高，但1 ， 3 -丙二醇濃度和生產強度有所下降。分享友人