考隆 的英文怎麼說
中文拼音 [kǎolōng]
考隆
英文
khao luong-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。It comprises, besides the brahmanic monuments built by the cholas, the monumental ruins of the fabulous garden - city created by parakramabahu i in the 12th century
在波隆納魯沃古城裡，不僅有考拉斯時期的婆羅門教遺址，還能看到帕拉克拉馬一世十二世紀時修建的神話般花園城市的遺跡。Qing scholars turned their attention to textual studies of the classics in the eighteenth century and established the qian - jia school of scholarship, making encouraging advances in the interpretation of words in confucian classics, textual criticism, philology, epigraphy and phonology
清代乾隆、嘉慶兩朝，考據之風盛行，訓詁、文字、金石、音韻等專門學科得到突破性發展，時稱"乾嘉學派" 。Through the investigation trip in rome ( italy ), vienna ( austria ), munich ( germany ), cologne ( germany ), frankfort ( germany ) and paris ( france ), the differences of fire prevention systems between europe and china are discussed
摘要通過考察義大利羅馬、奧地利維也納、德國慕尼黑、科隆和法蘭克福以及法國巴黎等城市的地鐵車站防災設施，探討了歐洲與我國防災系統的異同。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Problems and thoughts on mammalian somatic cloning
哺乳動物體細胞克隆存在的問題及其思考The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。If you are really into a fashion lifestyle, you should consider a disconnected, asymmetrical haircut, such as the one charlize theron wears in her new movie aeon flux
如果你的生活方式真的很入流，那麼你就該考慮來一個無層次感的非對稱發式，比如查理茲?塞隆在其電影新作《魔力女戰士》中的那種。It ' s the usual struggle. jerry sloan, sam mitchell, byron scott and maybe avery johnson most of all … you couldn ' t be wrong if you picked any of them
我總是要為此掙扎一番：傑瑞斯隆，薩姆米切爾，拜倫斯考特或許小將軍約翰遜。 。 。 。 。 。你選他們中的任一位都不為過。Long term disability pricing and profitability, looking beyond the calculations for better disability risk selection, underwriting and claims considerations for home - based workers, underwriting substandard disability insurance, underwriting income protection benefits, individual long term care as part of the individual disability insurance continuum, effective disability claims underwriting, and case management - transferring the skills to claims management
；超越計算，選擇更好的傷殘風險；針對在家工作者的核保和理賠考慮的因素；次標準傷殘險的核保；收入保障保險的核保-加費與除外條款；個人長期護理保險作為個人殘障保險的延續；有效的傷殘理賠-科隆再保險公司為德國人壽保險公司提供一項獨特的服務；案例管理：將案例管理技巧運用到理賠管理上。It is right to go together the industry to this gram that yuesheng breeze, yuesheng that designer that also seem to be to is very helpless, their speech : this kind of gram the s breeze ream they are proud of to again make them anxious about, the proud of is an own product to acquire the market with the approbation that go together, worry of is continuously for a long time hence, last in market positive development for having the yuesheng at considering yuesheng in the creation, style, disadvantage in go togethering the industry, let a hundred flowers blossom to is just spring. do not more lift under the watch elephant of the gram, the more is flow outing of appearance goods to counterfeit now, most the injure the consumer s benefits. the imitator is only meeting mimicry skin, copy not yuesheng creation is with the quality and to the vogue s sharp and full with confidence ability
對同行業對悅盛的這股克隆之風，悅盛的設計師也顯得頗為無奈，他們坦言：這種克隆之風令他們自豪又令他們擔心，自豪的是自己的產品獲得市場和同行的認同，擔心的是長此以往，市場上只有悅盛在思考悅盛在創造，風格趨同，不利於同行業的良性發展，百花放才是春。更別提在克隆的表象下，更多的是假冒偽劣的樣子貨的涌現，最終損害到消費者的利益。模仿者只會模仿皮毛，模仿不了悅盛的創造性和品質以及對時尚的敏銳把握能力。With an area of 5300 km2, dongpu depression is located in the southwest of lin - qing depression, bohaiwan basin, bounded with lu - xi uplift by lan - liao fault in the east, with nei - huang uplift by chang - yuan fault in the west, with lan - kao heave by the north feng - qiu fault in the south, with xin country depression by ma - ling fault in the north, while spreading along nne defection, taking on the forms of being wide in the south, and narrow in the north. it is a rift fault depression basin with the characteristics of striking, with the basement being the paleozoic and mesozoic, and the capping beds being the cenozoic
東濮凹陷位於渤海灣盆地臨清坳陷西南端，東側以蘭聊斷裂為界與魯西隆起為鄰，西側以長垣斷裂為界與內黃隆起相接，南以封丘北斷層為界與與蘭考凸起相鄰，北以馬陵斷層為界與與莘縣凹陷相望，是以古?中生界為基底，以新生界為蓋層，古近紀形成的拉分走滑?裂谷型箕狀斷陷盆地。What is it is going on in the world ? he asked himself in perplexity several times a day, instinctively beginning to sound the hidden significance in the phenomena of life. but knowing by experience that there was no answer to these questions, he made haste to try and turn away from them, took up a book, or hurried off to the club, or to apollon nikolaevitchs to chat over the scandals of the town
在一日之內他就有幾次惶惑不安地問自己，情不自禁地開始縝密思考生活中的各種現象的涵義，但他憑經驗也知道，這些問題都沒有答案，於是他趕緊設法迴避它，他時常看書，或者趕著上俱樂部，或者到阿波隆尼古拉耶維奇那裡去閑談市內的流言飛語。Fan sin temple is the main temple in the villages of the sheung wun yiu and ha wun yiu and is the only sample of this kind that exists in hong kong. the construction date of the temple is uncertain but a wooden plaque hanging at the main hall of the temple was carved in the geng - xu year of the qianlong reign of qing dynasty, indicating that the temple has a history of over 200 years
樊仙宮是上碗村和下碗村的主要廟宇，也是唯一在本港現存的該類廟宇，其建築日期已無從稽考，懸掛在正廳的木牌匾為清朝乾隆庚戍年間（一七九年）的雕刻，故相信該廟已有超過二百年歷史。Dai zhen ( 1723 ? 1777 ), xiuning county native in the qing dynasty, was a successful candidate in the imperial examinations at the provincial level in the reign of qianlong
戴震（ 1723 ? 1777 ） ：清休寧人，字慎修、東原，乾隆舉人，屢考進士不中，后開館纂修《四庫全書》 ，授翰林院庶吉士。On bronze mirror of qianlong design at the imperial palace in shenyang
沈陽故宮藏乾隆款銅鏡考According to the comprehensive survey on the development changes of the ideas about the subject, object, and the media of the tourism culture, we can divide the development course of china ' s tourism culture into six periods, namely the origin stage, the growth stage, the prosperity stage, the change stage, the dilemma stage and the transition stage
根據對旅遊文化的主體、客體和媒體以及旅遊觀念等發展變遷的綜合考察，可將中國旅遊文化的發展歷程劃分為濫觴、勃興、隆盛、新變、穴結和轉型等六個時期。And the fact shows both the high tide of textual researches in the society and zhu yi - zun ' s great influence on beijing scholars during the ruling of qianlong
從中可見戴震在治學方向上的自覺及考據學的高漲所引起的社會反映，同時也能看到朱彝尊在乾隆年間北京學人中的顯著影響。By tracing back to qian zai ' s quarrel with dai zhen after being enrolled into the imperial library, this essay probes into the origin of the disagreement with each other
摘要就戴震進四庫館后與錢載發生激烈爭執一事，追溯錢戴爭執的緣起，在乾隆三十一、二年，戴震曾因議論朱彝尊《經義考》而招致錢載極大不滿。Conservationist tenant preferred, but extractive forestry also considered. please apply to the cameroonian minister of forestry
保育人士優先考慮，但採掘業也會考慮。良機不再（ ） ，有意者請速向喀麥隆林業部申請。分享友人