肽基轉移 的英文怎麼說
中文拼音 [tàijīzhuǎnyí]
肽基轉移
英文
peptidyl transfer-
Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin
這些基因中有和肝再生有直接關系的如：胎球蛋白、組織蛋白酶；和肝臟功能密切相關的如：胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶；與物質能量代謝有關的如： atp合成酶、核糖體蛋白；以及與急相反應有關的如：觸珠蛋白、轉鐵蛋白。Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。Gene inductive expression of bifidobacterium on glutathione s - transferase in ht29 cell line
29細胞谷胱甘肽巰基轉移酶基因的誘導表達This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway
測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。Cloning and function characterization of glutathione s - transferase gene from tamarix androssowii
紫桿檉柳谷胱甘肽硫轉移酶基因的克隆及功能鑒定Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector, and e. coli bl21 was transformed by the recombinant plasmid for expression
Pcr產物經純化、酶切后，按正確的閱讀框架定向克隆到表達性載體pgex - 6p - 1中谷胱甘肽轉移酶（ gst ）基因的下游。Objective : polypeptide : n - acetylgalactosaminyl transferase is the initiation enzyme catalyzing the linkage of o - glucan chain. recent study shows that o - glucosylation is closely related to molecular recognition, tumor formation, development and metastasis, as well as embryonic development. due to the initial study on function of o - glucosylation in china, this thesis aims to obtain stably expressed pp - galnac - t2 gene clones for further study
目的多肽： n乙酰氨基半乳糖轉移酶是合成o糖鏈的起始酶，而目前的研究認為， o -糖基化與分子及細胞識別、腫瘤的發生發展和轉移以及胚胎發育等功能密切相關。The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。Two expression systems were used, one of which was qiaexpress system, the other glutathione s - transferase ( gst ) gene fusion system
）系統和谷胱甘肽s ?轉移酶（ gst ）基因融合表達系統。The research of gene expression condition of the glutathione s - transferase in escherichia coli bl21 de
含谷胱甘肽硫轉移酶基因工程菌表達條件研究分享友人