肽基部位 的英文怎麼說
中文拼音 [tàijībùwèi]
肽基部位
英文
p site-
In this paper, the relationship between cyanide - resistant respiration and expression of alternative oxidase ( aox ) using polyantibodies prepared by synthetic polypeptide in mung bean seedling, as well as the effects of vernalization on cyanide - resistant respiration and expression of aox in winter wheat were studied respectively. in the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic s helix of aox. were synthesized by solid - phase method
第一部分，我們按照交替氧化酶（ alternativeoxidase ， aox ）位於線粒體內膜外側親水區s螺旋的氨基酸序列，用固相法合成由12個氨基酸殘基組成的多肽，將此多肽與-牛胰凝乳蛋白酶原a相連，用作半抗原，免疫家兔制備人工合成12肽的抗體。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。Part iv computer - aided molecule modelling of tnfa mimotopes and tnfa - binding peptides : to investigate the interaction between tnfa mimotopes and tnfa - binding peptides, the computational docking program autodock ( with confirming calculations using discover ) was used to predict the binding modes of llt - 18 with tnfa firstly, then lcs - 7 was docked to llt - 18 by manual. the interaction between llt - 18 and tnfa or lcs - 7 showed electrostatic interaction and h - bond dominant
進行對接，對兩者間結合位點進行分析；在此基礎之上，以insight11軟體構建lcs刁分於三維結構， autodock程序對接llt和lcs 7兩個短肽分子，找尋兩者結合的關鍵性殘基，結果表明llt與tnfa及lcs 7分子間相互作用以靜電相互作用為主， lcs刀和tnfa活性部位的arg在分于間相互作用中起重要作用。The active site consists of amino acids arranged in a configuration specific to a particular substrate or type of substrate
構成酶活性部位的這些基團由於肽鏈的盤繞折疊使它們在空間結構上彼此靠近，形成具有一定空間結構的區域。分享友人