肽篩選 的英文怎麼說

中文拼音 [tàishāixuǎn]
肽篩選 英文
peptide screening
  • 肽 : 名詞[化學] （有機化合物） peptide
  • 篩 : 名詞[書面語] （植物名） sedge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 篩選 : dressing by screening; screen; preparation by screening; preparation; choose by means of a sift; ...
  1. Selection of ligand from a heptapeptide phage display library for lysozyme

    從七肽噬菌體展示庫中篩選蛋白質配基
  2. Screening of mimicry peptide of japanese encephalitis virus e protein from phage 15 - mer peptide library

    從噬菌體15肽庫中篩選乙腦病毒糖蛋白模擬肽
  3. The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )

    以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。
  4. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  5. The gene of amoa in ammonia - oxidizing encodes the active - site polypeptide of ammonia monooxygenase which catalyzes the oxidation of ammonia to hydroxylamine. we designed a pair of primers special for the amoa gene by comparing the known amoa gene sequences and used pcr to amplify the amoa gene fragments

    Amoa基因是編碼氨單加氧酶活性多肽位點基因，我們通過引物篩選合成了對氨氧化細菌amoa基因特異結合的引物序列，利用pcr技術對活性污泥中的amoa基因片段進行特異擴增，得到的dna片段大約為490bp 。
  6. By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq

    然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。
  7. Screening the antibody mimic peptide binding to hepatocellular carcinoma cells by phage display technique

    用噬菌體表面展示技術篩選與肝癌細胞系結合的抗體模擬肽
  8. The recombinant fasl - ecd was purified and its biological activity was analyzed on several cell lines and the most sensitive cell lines are selected. ( 2 ) using a computer program, a single short peptide is derived from antisense homology box of fas ligand and is chemically synthesized. ( 3 ) examine the apoptosis - inducing effect of the recombinant fasl - ecd and the ten - peptide on the most sensitive cell lines, and the relationship between them was analysed

    純化重組蛋白fasl - ecd ，並分析其生物學活性，篩選出對之最敏感的腫瘤細胞株； （ 2 ）根據生物信息學軟體分析結果，選取fasl胞外區256 - 265的十肽（ n ） - hlyvnvsels - （ c ）作為目標分子，委託生物公司合成該十肽； （ 3 ）分析fasl - ecd和十肽對最敏感腫瘤細胞的毒性作用，分析重組fasl一ecd及十膚作用的相關性。
  9. Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv

    方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。
  10. In order to obtain a new peptide antibiotics mutant molecule with strong antimicrobial activity and / or more simple structure, a mutant library was often constructed by genetical method and then the desirable mutant molecule was screened out from the library

    不同種類、不同突變體的肽抗生素活性有不同程度差異。為了獲得結構簡單、活性更強的肽抗生素，研究者們常常在克隆到肽抗生素分子后，通過構建其突變體庫，從中篩選更理想的目的分子。
  11. What is the most cost - effective strategy to screen for left ventricular systolic dysfunction : natriuretic peptides, the electrocardiogram, hand - held echocardiography, traditional echocardiography, or their combination

    篩選左心室收縮功能障礙最佳費用效益比的策略：鈉尿肽、心電圖、手持式超聲心動圖、傳統超聲心動圖或聯合應用這些方法？
  12. Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies

    小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選
  13. By far, there is no such reported research work as screening its prohibitors or ligands. our research work aims at constructing an expression system that could express bioactive ns5 protein and screening its small peptide ligands from which to single out its pioneer prohibitor

    本研究旨在建立denns5蛋白表達體系，獲得具有rdrp活性的重組表達產物；以ns5蛋白為靶分子，篩選相應的小分子結合肽，期望從中獲得具有rdrp活性抑制作用的先導物。
  14. In this study we screen the phage - epitope library in attempt to predict the receptor recognition site on vegf, identify peptide able to block the interaction of vegf - kdr and find a potent vaccine against tumor angiogenesis by targeting vegf

    [方法]以兩個具有中和vegf活性的抗- vegf單克隆抗體jh121 、 vg189為目標，分別對噬菌體隨機7肽、 12肽庫進行篩選。
  15. [ results ] all the selected clones gave strong signal in dot bloting and elisa

    [結果]對肽庫進行三輪篩選后，噬菌體克隆具有良好的富集效果。
  16. In this study, a novel strategy to inhibit the activation of the complement system has been developed. to get the purified clq as the target molecule for biopanning, the first step of our purification was to isolate the clq from clr2cls2 by affinity chromatography on igg - sepharose 4b column

    本研究另闢蹊徑，以補體經典激活途徑始動分子c1q為靶標，採用噬菌體肽庫技術，親和篩選能與c1q結合的噬菌體克隆，研製可抑制補體激活的c1q模擬短肽。
  17. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  18. Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa, for exploring whether the binding - peptides can be used as antagonist of tnfa or not

    篩選tnf小分子模擬肽及結合肽對于tnf研究具有重要的意義。本研究包括以下四個方面： 1 、 tnf結合肽的研究：以rhtnf為靶對噬菌體環七肽庫進行篩選，以尋求可拮抗tnf活性的小分子短肽。
  19. Immunoscreening of phage random peptide library with sera from patients with schistosomiasis japonica

    日本血吸蟲病患者血清對噬菌體隨機7肽庫的免疫篩選
  20. The antibody induced by peptide gwyydal abolished vegf - induced huvec growth in dose dependent which specifically express the kdr. moreover, both of the peptide gwyydal and vasavfysalve displaying on phage also inhibited the growth of huvec

    [結論]從噬菌體肽庫中篩選到了與抗體有高親和力的陽性噬菌體克隆，噬菌體表達的7 、 12肽gwyydal 、 vasavfysalve模擬了vegf的抗原表位，引起顯著的抗- vegf抗體反應。
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