肽質指紋圖譜 的英文怎麼說

中文拼音 [tàizhízhǐwéntúpǔ]
肽質指紋圖譜 英文
peptide mass fingerprinting
  • 肽 : 名詞[化學] （有機化合物） peptide
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 指 : 指構詞成分。
  • 紋 : 名詞（花紋; 紋縷兒） lines; veins; grain
  • 圖 : Ⅰ名詞1 （繪畫表現出的形象; 圖畫） picture; chart; drawing; map 2 （計劃） plan; scheme; attempt 3...
  • 譜 : Ⅰ名詞[書面語]1 （按類別或系統編成的書或冊子等） table; chart; register 2 （指導練習的格式或圖形）...
  • 指紋 : 1 （手指上的紋理） loops and whorls on a finger2 （指紋留下的痕跡） fingerprint; dactylogram; fin...
  1. For protein identification, proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised, digested in - gel with trypsin, and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ). erp60, trypsinogen, proelastase, lipaseandso on were identified. this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development

    從中隨機選擇一些差異蛋白質點，進行基質輔助激光解吸-電離飛行時間質譜（ maldi - tof - ms ）測定其膠內酶解后的肽質指紋圖譜，用mascot軟體查詢swiss - port數據庫，初步鑒定為一些與生長發育、物質代謝、細胞因子、信號轉導等有關的蛋白質，如erp60 、 trypsinogen 、 proelastase 、 lipase等。
  2. Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion

    差異蛋白經胰蛋白酶酶切后產生肽片段，再利用基質輔助激光解吸電離飛行時間質譜得到肽指紋圖譜來鑒定。
  3. 57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane. 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching. the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared

    二是通過原位酶解，抽提蛋白質片段進行maldi - tof質譜分析，利用肽質量指紋圖數據在數據庫中進行檢索，在雙向電泳凝膠上取了57個點進行分析，有34個蛋白質點在數據庫得到歸屬鑒定。
  4. In he - 3 and de - 3, 16 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 16 protein spots that were up - and down - r gulated were identified unambiguously. in he - 2 and de - 2, 20 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 5 protein spots that were up - and down - regulated were identified unambiguously, some of these different proteins are correlative with eyes development, nerve development, development regulation, cell differentiation and maintenance, arthromere formation and signal transduction. it shown that gene expression have significant difference between diploid and haploid during embryos developing

    分別選取其中70個分辨好的差異蛋白質點進行原位胰蛋白酶酶解，酶解的肽片段用基質輔助激光解析電離分行時間質譜（ maldi - tofms ）得到肽質指紋圖譜，再在網上peptident軟體中搜索swiss - prot和trembl數據庫，初步鑒定了he - 3和de - 3上32個差異蛋白質點，其中包括16個表達量上有差異的蛋白質點（ p 0 . 05 ）和16個有無表達的差異蛋白質點，並且初步鑒定了he - 2和de - 2上25個差異蛋白質點，其中包括5個表達量上有差異的蛋白質點（ p 0 . 05 ）和20個有無表達的差異蛋白質點。
  5. Methods and results : proteomics approaches involve three critical techniques : two dimensional electrophoresis, biological mass spectrometry and bioinformatics. in 2 - de, we applied traditional ief in the first dimension and tris - tricine system in the second dimension and acquired protein profiles of serum and skin samples. in the identification of 10 proteins of serum and 16 proteins of skin, we acquired peptide mass of fingerprint ( pmf ) maps for all targets by means of matrix - assisted laser desorption - ionization time of flight mass spectrometry ( maldi - tof - ms ) and partial aminio acid sequences for 9 proteins by means of electrospray ionization ms / ms ( lisi - ms / ms )

    研究方法：採用雙向電泳技術獲取泥鰍創傷前後血清和皮膚的小分子蛋白圖譜，利用基質輔助激光解析飛行時間檢測質譜分析（ matrix - assistedlaserdesorption - ionizationtimeofflightmassspectrometry ， maldi - tof - ms ）和電噴霧離子化串連質譜分析（ electrosprayionizationms / ms ， esi - ms hs ）分別獲得差異蛋白點的肽指紋圖譜（ peptidemassoffingerprint ， pmf ）和部分序列信息，通過網際網路上的expasy服務器中和ncbi的相關軟體將這些信息和swissprot數據庫進行匹配鑒定蛋白質種類。
  6. Proteins were identified with peptide mass fingerprinting ( pmf ). firstly, the first molecular anatomic profile of aeromonas hydrophila was established, 40 proteins were identitied from analysis of the pmf of l24 proteins. of the 40 proteins, 17 were from the source of aeromons hydrophila genes / proteins

    實驗首先初步構建了一張嗜水氣單胞菌總蛋白質的分子解剖圖譜，通過對124個蛋白質的肽質量指紋圖譜分析，鑒定了40個蛋白質，其中17個為嗜水氣單胞菌的蛋白。
分享友人
分享到 Line

分享到臉書