胚囊細胞 的英文怎麼說
中文拼音 [pēinángxìbāo]
胚囊細胞
英文
embryo sac cell- 胚 : 名詞[生物學] (初期發育的生物體) embryo
- 囊 : 囊名詞1. (口袋) bag; pocket; sack; purse 2. (像口袋的東西) anything shaped like a bag 1. (姓氏) a surname
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Before anthesis wall of egg apparatus is complete ; most part of egg wall at the chalazal end disappears at the day of anthesis ; after frtilization ( 20h after pollinating ) egg wall becomes intact. the results suggest that rebuilding of wall is one of the early cytological events during fertilization
結果表明,開花前藍豬耳的卵細胞的胞壁是完整的;開花時胚囊已發育成熟,此時卵細胞在合點端部分區域是裸的;受精后(大約授粉后20小時)卵細胞的胞壁恢復完整。Antipodal cells the three haploid cells found in the embryo sac of seed - bearing plants that migrate to the chalaxal end of the sac farthest from the micropyle
反足細胞:胚囊三次分裂形成卵細胞,助細胞和極核等,其中位於合點端的三個單倍體細胞稱反足細胞。Embryological studies reveal that megaspore and such cells as egg, synergid and antipodals in mature embryo sac initiate the division of forming haploid plants through embryogenesis or callus formation
胚胎學觀察揭示大孢子與胚囊內的卵細胞、助細胞和反足細胞均有可能在培養中啟動分裂,通過胚狀體或愈傷組織形成單倍體植株。Shortly after implantation the blastocyst wall, the trophoblast, consists of two layers of cells.
植入之後不久,胚囊的壁,即滋養細胞含有兩層細胞。Chromosome analysis confirmed that blastocyst cells were derived from es donor cell
重構囊胚細胞經染色體分析,證實其染色體來源於小鼠胚胎幹細胞。According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere
摘要從哺乳動物早期胚胎體外培養發育阻斷機理和早期胚胎的體內環境入手,闡述了胚胎體外培養各細胞階段胚胎對營養物質的需求,尋求合理的體外培養條件和方法,以便提高體外胚胎早期的囊胚發育率。Purpose embryonic stem cells ( escs ) which are from the inner cell mass ( icm ) of preimplantation blastula are pluripotent. neurons are considered as terminally differentiated cells that can not divide nor proliferate after injury
目的胚胎幹細胞( embryonicstemcells , escs )來源於著床前胚胎的囊胚期內細胞團( innercellmass , icm ) ,具有發育全能性。The fertilized egg entered the 4 - cell stage in 2hlomin, and the 8 - cell stage in 2h20min. the fertilized egg entered the 32 - cell stage in 3hl2min, and the cells distributed in different layers. the fertilized egg entered the multi - cell stage in 4h05min and the early blastula stage in 5h
1小時30分受精卵開始第一次分裂; 2小時10分進入4胞期; 2小時20分進入8胞期: 3小時12分進入32胞期,細胞出現分層現象; 4小時05分進入多細胞期; 5小時進入高囊胚期; 6小時50分進入低囊胚期。The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 (含2既的小牛血清,常規量雙抗)為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式(例如,從0一一6 ,凍存速度為一0 . 05 / min ,再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養,解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁,但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52
以細胞培養液m199 (含20 %的小牛血清)為稀釋液, dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣,採取先慢后快的方式,冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍,成活率為72 % ,第二組冷凍保存13天後解凍,成活率為520 / 0 。Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。Observation by paraffin section showed that lipeng no. 2 embryo sac mother cells were almost entirely degenerated during megasporogenesis
切片觀察發現,麗? 2號胚囊母細胞在四核期已完全退化。Eg cells of the 2th and 4th passage were akp ( alkaline phosphate ) positive. when cultured on degenerated feeder layers or in suspension, eg c ells formed embryoid bodies ( ebs ) in vitro
當eg細胞脫離飼養層懸浮培養,或在衰老的飼養層上延遲培養時,發現eg細胞或單個存在,或聚集成團,形成類似於早期胚胎的囊狀胚體結構The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性:雞胚尿囊液經離心、磷鎢酸負染后,電鏡觀察該病毒為典型的冠狀病毒;該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用,當繼代到第5代后,胚體嚴重病變;病毒在雞胚中隨著接種時間的延長,其效價增高, 96h可達到48h的2倍;該毒株可在cef上生長,但不能形成明顯的蝕斑;經1胰酶處理后可凝集雞紅細胞;雞胚的第四代尿囊液病毒回歸動物體,病死雞腎臟呈典型的花斑腎,腺胃則未見肉眼可見的病變。The mice showed also ageing - associated decline in oocyte quality, as indicated by increased frequency of premature chromatid separation, spindle disruption and / or chromosome misalignments in mii oocytes of aged mice. the gv oocytes from aged mice normally matured in vitro. the percentages of pronuclear - stage embryos, 2 - cell embryos and blastocyst derived from mii oocytes of aged mice by in vitro fertilization ( ivf ) were comparable with ones of young mice, but pronuclei formed 1 h later in aged mice than in young mice
老齡小鼠gv期卵母細胞能正常體外成熟;老齡小鼠m期卵母細胞經體外受精后形成原核期胚、 2 -細胞期胚和囊胚的比率與年輕小鼠比較無顯著差異,但原核形成時間延遲1小時;在老齡小鼠卵母細胞,注射牛精子抽提物或sr ~ ( 2 + )處理時所誘發的ca ~ ( 2 + )振蕩以及在減數分裂成熟及早期胚胎中的pkc分佈未發生改變。The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger
膽囊充盈、其內充滿稀薄的膽汁;法氏囊、胸腺萎縮,腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物;腎臟腫大。病理組織學變化方面,早期肝臟、腎臟、腸主要以出血、水腫和壞死為主,且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體,包涵體呈嗜酸性,為大型圓形包涵體或不規則的顆粒狀;法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡,法氏囊濾泡數目增多細胞間有較大空隙。The percentage of blastocysts in ml 6 medium with both taurine and edta ( 73. 6 + 2. 4 % ) was significantly higher than that in m16 with edta and taurine alone ( 60 + 1. 0 % and 61. 9 + 2. 0 %, respectively ). the change pattern in the cell parameters ( inner cell mass, icm ; total cell numb, tcn ) of the three groups was the same as the blastocyst formation rate
且m16 + tau + edta培養液中的囊胚總細胞數、內細胞團數、內細胞團與滋養層細胞數的比率均顯著高於m16 + tau和m16 + edta培養液的( p 0 . 05 ) ;內細胞團占總細胞數的百分率也相應增加。Since the first embryonic stem cell derivation from mouse blastocyst by british scientists in 1981, groups of scientists were devoted into the research field of stem cells. in 1998, two scientific groups of america obtained human embryonic stem cells and human embryonic germ cells respectively from human blastocysts and gonadal ridge of early embryos
1998年,美國的兩個科學家小組分別從人體外受精發育而成的囊胚內細胞團和5 9周齡流產的胚胎原始生殖嵴中,成功獲得了人類胚胎幹細胞系和人類生殖嵴幹細胞系。Based on these observations, we isolated protoplasts from female germ unit and performed extracellular ca2 + influx experiment in order to check whether the cytological event similar to what happens during fertilization will be induced by extracellular ca2 + influx
表明細胞壁的合成是受精后早期發生的細胞學事件。在此基礎上,我們分離了胚囊細胞的原生質體。通過外源導入鈣離子檢查鈣能否引發類似受精過程發生的細胞學事件。Although the cell parameters ( icm and tcn ) of blastocysts of both groups differed significantly ( p < 0. 05 ), the mml 6 + e2 + p group was the closest to the in vivo group, and those of the mm16 group were the lowest
同時添加e2和p的mm16培養液中囊胚的細胞總數、內細胞團數、內細胞團占總細胞的百分率和內細胞團與滋養層細胞數的比率低於體內正常發育囊胚,但顯著高於其它體外培養組( p 0 . 05 ) 。分享友人