胚後期的 的英文怎麼說
中文拼音 [pēihòuqīde]
胚後期的
英文
postembryonic-
The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 (含2既的小牛血清,常規量雙抗)為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式(例如,從0一一6 ,凍存速度為一0 . 05 / min ,再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養,解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁,但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52
以細胞培養液m199 (含20 %的小牛血清)為稀釋液, dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣,採取先慢后快的方式,冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍,成活率為72 % ,第二組冷凍保存13天後解凍,成活率為520 / 0 。Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %
採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵,實驗結果如下: ( 1 )顯微注射后,根據胚胎發育分期,胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光,原腸期發熒光的胚胎比例為25 ,後期發育熒光率逐漸下降,肌肉效應期后又相對穩定。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件,以hprt為陽性對照,用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析,結果發現,轉錄sry基因的睪丸組織以及雄性胚胎,從受精卵發育到囊胚的過程中,基本上不轉錄xist基因;不轉錄sry基因的雌性卵母細胞和雌性胚胎,從出現原核開始,到發育至2 -細胞期的過程中, xist基因一直不轉錄,但是,從4 -細胞期開始,一直到孵化前囊胚階段,雌性胚胎都轉錄xist基因。The valt and the p valt of cyanide - resistant respiration were induced dramatically as well as the total respiration in early stage of vernalization treatment and deceased subsequently. the western blotting results also showed the parallel changes. during the whole treament time of 0 - 30d, although the cyanide - resistant respiration was increased, its opearation was lower than the cytochrome pathway which also made the main contribution to total respiration of winter wheat
結果表明:冬小麥胚芽在春化處理初期,伴隨著總呼吸速率的上升,抗氰呼吸容量( valt )和實際運行活性( pvalt )也快速升高,但在春化後期兩者均呈下降趨勢,與westernblotting結果十分吻合;在整個春化處理期間,抗氰呼吸活性雖有所上升,但相對于細胞色素主路來說,其運行還是處在一個較低的水平,而細胞色素主路則仍然占據了優勢。With the increase of substrate salinity, the accumulated sodium and chloride increased. as a result, all tissues had considerablly lower osmotic potentials than that of the solution on which they were grown at 60 day after planting. changes in length, dry weight, water content, ion concentrations, osomotic potential, ion content of hypocotyls during culture indicated that viviparous hypocotyls not only afforded nutrition for seedling growth, but also reserved ions, thus charged the balance of ion concentration and osmotic potential of the seedling
鹽脅迫下幼苗單株葉面積下降的程度大於光合速率的降低,葉面積的減小是導致減產的主要原因;木欖幼苗各組分中的離子濃度以及含量隨栽培時間而變化;栽培初期剛萌根時,幼苗原胚軸中的離子滲漏到培養液中;此後隨著根系的發育以及芽的生長,幼苗轉為從培養液中吸收離子,並以吸收na 、 cl離子為主。The first period is the embryonic period ( from around the usa was found to the middle of the 1960s ), the second period is the initial period ( from the middle of the 1960s to the end of the 1980s ), and the third is the flourishing period ( from the end of the 1980s to this day )
筆者將美國擇校制度的發展歷程分為三個階段,即美國擇校制度的胚胎期(建國前後至1960年代中期) 、美國擇校制度的初步形成期( 1960年代中期至1980年代末期)和美國擇校制度的蓬勃發展期( 1980年代末至今) 。Following the embryonic stage of development
胚胎後期的在胚胎發育期之後的Stem ce11 factor is a multipotent cytokine which is found in recent years, fol1owing research in vitro and in vivo found that scf and its receptors have familiar regu1ation function in mature brain with in embryo and developing period
幹細胞因子是近年來發現的一種多功能細胞因子。近年來研究發現,幹細胞因子( stemcellfactor , scf )及其受體對胚胎期及成年後神經系統生長發育起調控作用,但其作用機制尚不明了。Using anatomical method, the gonadal development in postembryonic period of rana chensinensis, which had been migrated from northeast china, is studied
摘要採用解剖學方法,對南移后的中國林蛙胚胎後期性腺發育進行了觀察。The results showed that the gonadal development in postembryonic period of rana chensinensis was well - balanced, and the environment of guangdong is adapted to the gonadal development
結果表明,南移后的中國林蛙胚胎後期的性腺發育基本正常,環境的變化並未對其產生不良影響。The effect of temperature on the late embryonic development of the toad, bufo raddei
不同溫度對花背蟾蜍後期胚胎發育的影響Although earlier attempts at retina transplantation using embryonic stem cells failed, the researchers say that by using stem cells at a later stage of development, they were able to repair damaged retinas in the mice
盡管以前嘗試使用胚胎幹細胞進行視網膜移植失敗了,研究人員稱通過使用發育後期的幹細胞,他們修復了小鼠受損的視網膜。“ we worked on the theory that cells at a later stage of development might have a higher probability of success upon transplantation ” says researcher robin ali of the university college london institute of ophthalmology in a news release
盡管以前嘗試使用胚胎幹細胞進行視網膜移植失敗了,研究人員稱通過使用發育後期的幹細胞,他們修復了小鼠受損的視網膜。Method of metaphase chromosome preparation from scallop was optimized. the gill tissue and embryo before the stage of trochophoro larve were proved to be good materials for chromosome preparation, while excellent chromosomes can hardly be prepared from the gonad tissue or embryo after the stage of trochophoro larve
確定成體扇貝鰓絲或者擔輪幼蟲期的早期胚胎是較為優良的染色體制備取材來源,而性腺材料、進入d型幼蟲期以後的扇貝胚胎則較難以制備優良的染色體分裂相。This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
該載體的特點是:具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區;將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後,而且沒有增加額外的序列和使人血清白蛋白cdna移碼;引入標記基因gfp ,便於在胚胎期鑒定陽性胚胎,減少受體;引入cre lox重組系統: ( ? )標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后,刪除標記基因; ( ? )餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。Ss - ir cells, 5 - ht - ir cells, pp - ir cells, tgf p 1 - ir cells and sp - ir cells were found in the esophagus, the stomach and the duodenum of alligator sinensis embryos
在胚胎發育後期,消化道中以胃的內分泌細胞數量和種類最多,也是內分泌調控最重要的部位,其次是十二指腸。The postembryonic development of rana chensinensis which had been moved to south china were first studied in this paper
摘要首次對中國林蛙在華南地區的胚胎後期發育進行觀察研究。The results showed that the environment of south china adapt to postembryonic development of the rana chensinensis and the higher temperature had no effects on the growth but it accelerated the embryonic development speed
結果表明:華南地區的環境條件基本適合中國林蛙的胚胎後期發育,較高的溫度加快了中國林蛙的胚胎發育進程,但對其生長尤明顯影響。The ability to clone embryos was perfected11 by scientists in scotland in the late 1990s and the ability to grow stem cells into tissues and organs is being developed12 by american researchers
克隆胚胎的技能在20世紀90年代後期由蘇格蘭科學家研究成功並日臻完善;把幹細胞培養成組織和器官的技術正由美國研究人員加以開發。分享友人