胚性結織 的英文怎麼說
中文拼音 [pēixìngjiēzhī]
胚性結織
英文
embryonic connective dissue-
Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件,以hprt為陽性對照,用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析,結果發現,轉錄sry基因的睪丸組織以及雄性胚胎,從受精卵發育到囊胚的過程中,基本上不轉錄xist基因;不轉錄sry基因的雌性卵母細胞和雌性胚胎,從出現原核開始,到發育至2 -細胞期的過程中, xist基因一直不轉錄,但是,從4 -細胞期開始,一直到孵化前囊胚階段,雌性胚胎都轉錄xist基因。Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter. lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv, raise the level of serum antibody. they make chickens obtaining strong immunocompetente in a short time. agp liters of the group of infecting 0. 4mlcbs + ibdv of germinal and 0. 8mlcbs + ibdv of cellcular or 0. 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 liters averagely. the chickens were inoculated with ibdv live vaccine mixed with the different dosages of lyophilized bursin by the eye drop method. the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv. they may also alleviate the immunological injury of activated virus to bursa of fabricius. and promote the repairation of the lesion. it can be found that bs can raise body weight gain and feed coversion ratio
將超濾獲得的法氏囊活性肽分別以不同劑量肌肉注射21日齡spf雞,同時頸部皮下注射ibd胚毒或細胞毒滅活苗,結果表明:雞、鴨法氏囊活性肽都能夠縮短ibd油苗誘導產生抗體的時間,提高抗體水平,使雞可以在比較短的時間內獲得堅強的免疫。 0 . 4mlcbs胚毒組和0 . 8mlcbs細胞毒組或0 . 8mldbs胚毒和細胞毒組的agp抗體滴度平均比免疫對照組高2個滴度。將法氏囊活性肽與ibd活苗聯合免疫雞,結果表明:不同劑量的cbs和dbs都可以對不同日齡雞ibd抗體的產生有不同程度的促進作用;還可以減少弱毒對雞法氏囊組織的損傷,加快其修復。The endocrine cells in the digestive and glands of alligator sinensis embryos aged from 8th to 55th day were localized and compared by using immunohistochemical method with thirteen kinds of antiseras of hormone. during the development of pancreas in alligator sinensis embryos, somatostatin ( ss ) immunoreactive ( ir ) cells, 5 - hydroxytryptamine ( 5 - ht ) - ir cells, glucagon ( glu ) - ir cells, epidermal growth factor ( egf ) - ir cells appeared on 18th day. no p53 protein - ir cell, gastrin - ir cell, testosterone - ir cell, chromogranin a - ir cell, vasoactive intestinal polypeptide - ir cell, epithelial membrane antigen - ir cell or insulin - ir cell was found in the pancreas of alligator sinensis embryos
本實驗採用免疫組織化學技術,應用13種不同的抗血清,對孵育時間8 ? 55天揚子鱷胚胎消化道及消化腺內分泌細胞的種類進行鑒別、定位和比較,結果如下:揚子鱷胚胎胰腺中,生長抑素、 5 ?羥色胺、胰高血糖素、表皮生長因子、胰多肽免疫反應陽性細胞出現于第8天; p物質免疫陽性細胞出現于第18天; p53 、胃泌素、睪酮、嗜鉻素a 、血管活性腸肽、上皮膜骯原、胰島素免疫陽性細胞在各期揚子鱷胚胎胰腺中均未發現。The difference between these 2 groups was statistically significant. conclusions the results suggested that the genesis of nephroblastoma is relevant to the retarded differentiation of fetal kidney
結果腎母細胞瘤與早期胚胎腎在組織形態上有相似之處; 22例腎母細胞瘤瘤旁腎組織中6例見腎源性剩餘存在, 30例非腫瘤成熟腎組織中僅1例見腎源性剩餘存在。The main results are as follows : ( 1 ) plant regeneration system of tall fescue embryogenic calli was established according to studies on impacts of manifold factors on callus induction from mature seeds and subculture production and differentiation of embryogenic calli
主要研究結果如下: ( 1 )通過對成熟種子愈傷組織誘導、胚性愈傷組織繼代發生和分化的多種影響因素的研究,建立了高羊茅胚性愈傷組織植株再生體系。Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle, immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens, the transformation rate of the above acceptor was investigated respectively. the results showed that immature embryo after precultured for 4 ~ 6d was the best. in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb, to agrobacterium tumefaciens eha 105, od value of 0. 8 was the best
採用農桿菌介導法將bt毒蛋白基因導入水稻同樣以上述兩種秈稻為主要研究材料,比較了分別以預培養的幼胚和幼穗、幼胚、成熟胚來源的愈傷組織作為轉化受體的愈傷組織轉化頻率,結果表明預培養4 6天的幼胚最適宜作為農桿菌介導轉化的受體;其次是來源於幼胚和成熟胚的生長狀態良好的胚性愈傷組織。The results showed that, at 12 48 hrs post infection, the bursal epithelial cells bore swollen and necrotic shape. the lymphoid cells in the medulla and cortex of lymphoid follicles had various degrees of degeneration and necrosis. the lymphoid follicles showed glandular structures or cystic vacuoles owing to the prolifetation of bursal epithelium and lymphocyte depletion. lymphoid follicles from bursa at 72 144 hrs post inoculation became atropy. marked proliferation of interfollicular and interstitial connective tissue was present
結果表明,病毒感染后12 48小時,雛雞法氏囊粘膜上皮細胞腫脹、壞死脫落,淋巴濾泡髓質部及皮質部淋巴細胞不同程度變性、壞死、排空,形成腺管樣結構或囊狀空泡,接毒后72 144小時,法氏囊淋巴濾泡淋巴細胞壞死排空,淋巴濾泡萎縮,網狀結締組織大量增生,而胚胎發育時期,法氏囊粘膜上皮腫脹變性,法氏囊淋巴濾泡形成延遲或不完整,淋巴濾泡內淋巴細胞缺乏或空虛。The expression patterns of soluble proteins, peroxidase and esterase isozymes were analysed on globular embryogenesis in tissue cultures of alfalfa, it was concluded that from embryogenic callus to globular embryo genesis h peptides emerged and disappeared orderly, the patterns of peroxidase isozyme changed dramatically, esterase isozymes changed slightly while the total enzyme activity was important to keeping the potential of somatic embryogenesis
摘要試驗在苜蓿組織培養中,對球形胚形成過程中特異蛋白質表達的模式、過氧化物酶及酯酶同工酶酶譜變化進行研究,結果表明:苜蓿組織培養中從胚性愈傷組織到球形胚發育的進程中,順序消失和出現了11種中小分子量多肽;過氧化物酶同工酶酶譜發生了顯著的變化;酯酶同工酶酶譜變化不大,但其總活力對于維持體細胞胚胎發生是必須的。The results showed that the levels of endogenous polyamines in embryogenic calli were higher than those in non - embryogenic calli ; the changes of endogenous polyamines in the process of somatic embryogenesis approximately showed an " m " curve during embryogenic callus and globular embryoids ; the content of polyamines was the 2 turning - point stages in the changes of endogenous polyamines in somatic embryogenesis ; the changes of endogenous polyamines were earlier than those of morphogenesis
結果表明:龍眼胚性愈傷組織中內源多胺含量比非胚性愈傷組織高;體胚發生過程中內源多胺含量變化趨勢大致呈" m "狀,在胚性愈傷組織和球形胚階段轉折;內源多胺含量的變化先於形態學的變化。分享友人