胚細胞的 的英文怎麼說
中文拼音 [pēixìbāode]
胚細胞的
英文
protoblastic- 胚 : 名詞[生物學] (初期發育的生物體) embryo
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 的 : 4次方是 The fourth power of 2 is direction
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Metabolic activity quickly develops in the cells of the embryo and the aleurone layer.
胚細胞和糊粉層中的代謝活性立即活躍起來。Before anthesis wall of egg apparatus is complete ; most part of egg wall at the chalazal end disappears at the day of anthesis ; after frtilization ( 20h after pollinating ) egg wall becomes intact. the results suggest that rebuilding of wall is one of the early cytological events during fertilization
結果表明,開花前藍豬耳的卵細胞的胞壁是完整的;開花時胚囊已發育成熟,此時卵細胞在合點端部分區域是裸的;受精后(大約授粉后20小時)卵細胞的胞壁恢復完整。The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 (含2既的小牛血清,常規量雙抗)為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式(例如,從0一一6 ,凍存速度為一0 . 05 / min ,再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養,解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁,但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。It accumulates in the amyloplasta of the endosperm cells during grain filling.
在穀粒灌漿時,它在胚乳細胞的造粒體中積累。A primary culture of myoblasts may be prepared by excising skeletal muscle tissue from 10 to 30 day old rat embryos.
制備成肌細胞的初級培養物,可以將10-30天的大鼠胚胎骨骼肌組織剪切下來。This pushing-in of cells marks the start of gastrulation.
細胞的這種向里推進標志著原腸胚形成的開始。( 3 ) the chromosomes obtained by air - drying and chromosomes from the silkworm genital gland are similar in shape. but the numbers and the length of the chromosomes are obviously different. the chromosomes obtained from the genital gland have little difference in length, while the chromosomes of bmn cell are different in length very much
但是,在染色體的數目上有顯著的差異。另外,染色體間的長短差別也非常明顯。由家蠶生殖腺得到的減數分裂中期染色體和胚胎得到有絲分裂的中期染色體,其染色體長短差異不明顯,而binn細胞的中期染色體長短非常不一致,差異非常顯著。Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。Protein kinase a is camp - dependent kinase, one of the most important signal transduction pathways, plays a pivotal role in growth, differentiation, tumor occur, cell cycle control, etc. pka activators such as camp, 8 - br - camp or phosphodiesterase ( pde ) inhibitor isomethyl butyl xanthine ( imbx ) or purified pka catalytic subunit all can inhibit germinal vesicle breakdown ( gvbd ) and meiotic maturation in mouse oocytes, also in xenopus oocytes
蛋白激酶a ( proteinkinasea , pka )是依賴于camp的絲蘇氨酸蛋白激酶,是重要的信號傳導途徑之一,廣泛參與許多生命過程,包括生長、分化、腫瘤發生、細胞凋亡、細胞周期調控等。以小鼠卵母細胞為實驗對象,給予pka激動劑camp 、 8 - br - camp或磷酸二酯酶抑制劑imbx ( isomethylbutylxanthine )均可抑制小鼠卵母細胞的胚泡破裂及減數分裂成熟。The purpose of this study was to establish es or eg cells lines in the future for further research. the results attained were as follows : 1. derivation of embryonic stem cells from icr mouse preimplantation embryos
本試驗對icr小鼠胚胎多能幹細胞的建系工作做了初步探索,為胚胎多能幹細胞多種屬廣泛建系的研究打下一定基礎,也為小鼠胚胎多能幹細胞的深入研究積累經驗並提供借鑒。2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料,經傳代培養,獲得能連續而穩定傳至6代的,具有胚胎幹細胞諸多特性的eg細胞系。At the same time, growth factors have relation to genesis of tumor. fibroblast growth factor ( fgf ) can promote proliferation of tissues derived from mesoderm or neuroectoderm and many tissues and cells can secrete fgf
成纖維細胞生長因子( fgf )能夠誘導中胚層和神經外胚層來源細胞的增殖和分化,許多組織和細胞都可以分泌fgf , fgf可以促進多種細胞的生長和分化。7 - 10 days " seedlings after the seeds germinated, the meristem in the stem apex functioned to form true leaf primordium, meanwhile, the vessel elements which differentiated from parenchymatous cells of the middle and upper portion of the cnz connected with the vessel elements from procambium of epcotyl - shoot region
種子萌發7 - 10天,幼苗的莖端分生組織活動形成真葉雛形,此時由子葉節區中、上部薄壁細胞的轉分化形成的導管分子與上胚軸-苗區原形層形成的導管分子發生連接。This cytoplasmic terrain, which is the progenitor of the cytoplasm of the embryo, is designated as neocytoplasm.
這個細胞質范圍是胚細胞質的先質,它被稱為新細胞質。The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger
膽囊充盈、其內充滿稀薄的膽汁;法氏囊、胸腺萎縮,腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物;腎臟腫大。病理組織學變化方面,早期肝臟、腎臟、腸主要以出血、水腫和壞死為主,且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體,包涵體呈嗜酸性,為大型圓形包涵體或不規則的顆粒狀;法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡,法氏囊濾泡數目增多細胞間有較大空隙。The differential diagnosis of brainstem tumor for a patient with this age includes astrocytoma, intramedullary ependymoma, medulloblastoma and choroid plexus papilloma
在該年齡層的鑒別診斷為星細胞瘤,髓腔內室管膜瘤,神經管胚細胞瘤及脈絡叢乳頭狀瘤。- 10 - ivm oocytes were fertilized in vitro, and the process of fertilization, early cleavage, and blastocyst formation were recorded
通過體外受精觀察比較各組體外成熟卵細胞的受精率、卵裂率和囊胚形成率: 6In addition, insulin - ir cell, chromogranin - a - ir cell, testosterone - ir cell and epithelial membrane antigen - ir cell were not detected in the digestive tract of alligator sinensis embryos. the kinds and the numbers of endocrine cells in the digestive tract and glands were less at early prenatal stages but increased greatly in the midterm of development. in the digestive tract of last prenatal stages, it was stomach that had the most endocrine cells and might play the most important role in regulating the growth and differentiation of the digestive tract, the duodenum was in the second place
縱觀揚子鱷胚胎消化道和消化腺內分泌細胞發生的情況,可得出如下結論: )胚胎發育早期,胰腺內分泌細胞的數量和種類較多,可能是早期胚胎重要的內分泌調控部位;胚胎中期消化道和消化腺內分泌細胞的數量和種類增加最多,是內分泌細胞分化發育的重要時期:胚胎晚期,以胰腺和幽門、十二指腸的內分泌細胞最密集,為孵出后的消化和吸收的功能調控做好生理準備。The endocrine cells in the digestive and glands of alligator sinensis embryos aged from 8th to 55th day were localized and compared by using immunohistochemical method with thirteen kinds of antiseras of hormone. during the development of pancreas in alligator sinensis embryos, somatostatin ( ss ) immunoreactive ( ir ) cells, 5 - hydroxytryptamine ( 5 - ht ) - ir cells, glucagon ( glu ) - ir cells, epidermal growth factor ( egf ) - ir cells appeared on 18th day. no p53 protein - ir cell, gastrin - ir cell, testosterone - ir cell, chromogranin a - ir cell, vasoactive intestinal polypeptide - ir cell, epithelial membrane antigen - ir cell or insulin - ir cell was found in the pancreas of alligator sinensis embryos
本實驗採用免疫組織化學技術,應用13種不同的抗血清,對孵育時間8 ? 55天揚子鱷胚胎消化道及消化腺內分泌細胞的種類進行鑒別、定位和比較,結果如下:揚子鱷胚胎胰腺中,生長抑素、 5 ?羥色胺、胰高血糖素、表皮生長因子、胰多肽免疫反應陽性細胞出現于第8天; p物質免疫陽性細胞出現于第18天; p53 、胃泌素、睪酮、嗜鉻素a 、血管活性腸肽、上皮膜骯原、胰島素免疫陽性細胞在各期揚子鱷胚胎胰腺中均未發現。The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body
小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑,其制備、傳代及冷凍保存均有不同的研究報道,飼養層的質量受許多因素的影響,如動物的品種、培養液、所傳代數及實驗條件等,關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少,故本實驗以昆明小白鼠為實驗動物,研究其胚體培養過程中細胞的形態學、組織學等方面的變化,以期為本實驗室建立飼養層細胞庫提供理論依據,同時探討心肌細胞和成纖維細胞層共培養的可行性,以期為心臟生物學特性的體外研究奠定基礎。分享友人