For the cryogenic preservation of fish, in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp, the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture, the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made
本文主要從魚類種質保存的目的出發,一方面以四倍體鯽鯉魚為材料,對四倍體鯽鯉魚
腎臟組織進行初步培養,為建立相應細胞庫及下一步培養凍存的魚類
胚胎細胞奠定基礎;另一方面,以魚類組織細胞培養技術為基礎,泥鰍
胚胎細胞為材料,對魚類
胚胎細胞凍存培養方法進行初步研究,並應用該技術方法對草魚早期
胚胎細胞進行凍存培養實驗。報告如下:本文用組織貼壁法對四倍體鯽鯉魚
腎組織進行原代、傳代培養。
In this stlidy, g5 serotype a gidup poreine rotavha stanha strain osu was propagated on ma - l04 monolayer cell
本研究用恆河猴
胚腎細胞( ma - 104細胞系)繁殖並擴增了a群prvg5型標準毒株osu 。
The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger
膽囊充盈、其內充滿稀薄的膽汁;法氏囊、胸腺萎縮,腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物;
腎臟腫大。病理組織學變化方面,早期肝臟、
腎臟、腸主要以出血、水腫和壞死為主,且肝細胞核及
腎小管的上皮細胞核內均發現有核內包涵體,包涵體呈嗜酸性,為大型圓形包涵體或不規則的顆粒狀;法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞
胚肝細胞、
腎小管上皮細胞的胞漿內出現1 2各大的空泡,法氏囊濾泡數目增多細胞間有較大空隙。
These are normal term infant kidneys. note the fetal lobulations and the smooth cortical surfaces with some attached adipose tissue
正常足月兒
腎臟,可見
胚胎期
腎小葉;光滑的皮質表面有脂肪組織。
The difference between these 2 groups was statistically significant. conclusions the results suggested that the genesis of nephroblastoma is relevant to the retarded differentiation of fetal kidney
結果
腎母細胞瘤與早期
胚胎
腎在組織形態上有相似之處; 22例
腎母細胞瘤瘤旁
腎組織中6例見
腎源性剩餘存在, 30例非腫瘤成熟
腎組織中僅1例見
腎源性剩餘存在。
2 cell line and tissue sample : the human gastric cancer cell line sgc - 790land human embryo kidney cell line hek - 293 cell were kept in cell biolo - gy laboratory
2 .實驗材料:人胃癌細胞系sgc一7901 、人
胚腎細胞系hek一293由本室所存。
We mainly to cured on the mechanism of kidney development, inducing of embryonal kidney development in vivo as well as the status in quo of using stem cells in kidney diseases therapy
包括:體外誘導
胚腎組織發育;體外誘導幹細胞向
腎臟細胞的分化;骨髓、成體
腎和
胚胎
腎來源的幹細胞用於
腎臟疾患治療的研究。
Pathologic study revealed a cystic nephroma with foci of primitive, mesenchymal, undifferentiated, embryonal type sarcoma
病理上顯示為一囊腫性
腎細胞瘤合併局部呈現原生間質性,未分化的,
胚胎性的肉瘤變化。
Observation of chicken phenotype and rt - pcr results of tyrp1 expression in 8 day, 16 day embryos and 2 day hatched chicken also showed that melanin accumulation was appeared after detection of tyrp1 in silky. meanwhile, tyrpi expression was only detected in eyes of white leghorn ( except for kidney ), the only tissue where melanin exists
Rt - pcr檢測白萊航和烏骨雞的8天、 16天
胚胎和出生后2天各組織的tyrp1表達,結合對表型的觀察發現,烏骨雞各組織中黑色素的沉積隨tyrp1的表達出現,而白萊航(除
腎臟外)只在有黑色素沉積的眼睛中表達tyrp1 ,其他組織既無tyrp1的表達也無黑色素沉積。
Metanephric adenoma is a unique renal tumor with benign biologic behaior in spite of their large size
后
腎腺瘤盡管體積較大,但卻為
胚胎發育過程中后
腎來源的良性腫瘤,較少見。
A further analysis using human embryonic tissues ( 16 to 24 weeks ) showed a development - specific expression pattern in heart, skeletal muscle, liver, lung, kidney and brain suggesting a role for these genes in embryonic development
胚胎組織( 16周- 24周)的northern雜交結果表明隨著發育階段的不同,以上四個基因在心臟,骨骼肌,肝,肺,腎和大腦中的表達量有一定的變化,這說明它們在胚胎發育過程中起著一定的作用。