胞病毒菌素 的英文怎麼說
中文拼音 [bāobìngdújūnsù]
胞病毒菌素
英文
cytovirin- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 素 : Ⅰ形容詞1 (本色; 白色) white 2 (顏色單純) plain; simple; quiet 3 (本來的; 原有的) native Ⅱ名...
- 病毒 : [醫學] virus; inframicrobe (濾過性)
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The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ) it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中,細菌污染是影響疫苗質量和產量的關鍵性因素,筆者通過了兩年左右的時間,選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份,進行細菌學檢查,並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗,結果表明:我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌,潛在威脅是桿菌和鏈球菌;強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果,可作為疫苗生產后備抗菌手段參考Plant defensin disrupts the cell membrane of phytopathogen differently from cema that contacts directly with the cell membrane. plant defensins are thought to interact with specific receptor harboring in the cell membrane
由於對真菌,特別是絲狀真菌有很強的抑制作用,而對植物細胞沒有毒害作用,防禦素有望在植物抗病基因工程中得到廣泛應用。In terms with the principle of fusarium oxysporiun caused plant disease : bundles were blocked and fusarid acid killing cells was formed by hyphae so that caused water metabolism abnormal and plant wilting. in order to find out effective method of anti - fiisarium oxysporuin, long ya lillium was taken as material with plant tissue culture and genetic transformation techniques in this paper
針對尖孢鐮刀菌的致病機理:菌絲阻塞維管束引起水分代謝失常和菌絲在植物體內產生毒素(鐮刀菌酸)損害膜結構造成代謝失常,從而導致植物萎焉。本實驗以龍牙百合為研究對象,應用細胞工程中的離體培養方法並結合轉基因技術,以期找到抗尖孢鐮刀菌的有效途徑。Acellular as well as inactivated polioviruses types i, ii iii. the old dtwp vaccine is also a combination vaccine with similar components for diphtheria and tetanus but contains the " whole - cell " component for pertussis and does not contain the poliovirus component
新疫苗的白喉及破傷風類毒素成份與以往所用的三合一白喉破傷風百日咳混合疫苗dtwp相若,而以往所用疫苗的百日咳桿菌為全細胞型及不含小兒麻痹病毒。The crystalline immune system is a more complex structure of 18 forms of white blood cells, each of which has the capacity to destroy and consume various toxic substances from waste matter to viruses to bacteria that would destroy the form otherwise
水晶免疫系統是一種更復雜的結構,有18種白血細胞,每一種都具有消滅并吞噬從來自廢物的不同毒素直到各種病毒細菌的功能,否則它們就會摧毀身體。The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction
目的基因經雙酶切后連接載體ppic9k ,然後導入大腸桿菌dh5中,在含氨卞青霉素( amp )的lb板上用pcr反應篩選出陽性菌落,雙酶切結果表明目的基因已插入載體中,且方向正確,測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。Solutions of povidone - iodine gradually release iodine to exert an effect again bacteria, fungi, viruses, protozoa, cysts and spores
碘射素是碘的有機聚合物,其水溶后慢慢游離碘能有效地殺死細菌黴菌病毒原蟲包囊及芽胞。For example, as a direct toxic effect, periodontal iruses and bacteria and their products ( endotoxins or enzymes, for example ) are toxic to surrounding cells and may directly induce mutations in tumor suppressor genes and proto - oncogenes or alter signaling pathways that affect cell proliferation or surial of epithelial cells
例如,作為直接毒性作用,牙周病毒和細菌及在細胞周圍其產生的毒性物質(例如,類毒素或酶類)可直接誘導腫瘤抑制基因和原癌基因突變或者改變細胞信號傳導通路從而影響上皮細胞增殖或存活。For example, as a direct toxic effect, periodontal viruses and bacteria and their products ( endotoxins or enzymes, for example ) are toxic to surrounding cells and may directly induce mutations in tumor suppressor genes and proto - oncogenes or alter signaling pathways that affect cell proliferation or survival of epithelial cells
例如,作為直接毒性作用,牙周病毒和細菌及在細胞周圍其產生的毒性物質(例如,類毒素或酶類)可直接誘導腫瘤抑制基因和原癌基因突變或者改變細胞信號傳導通路從而影響上皮細胞增殖或存活。Produced by pseudomonas syringae pv. atropurpurea, a pathogen of oats and pseudomonas syringae pv. glycinea. the toxin causes chlorosis and streaking in oats
丁香假單胞菌的兩個致病變種可產生此毒素,這種毒素造成褪綠和條點。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系,本實驗研究分為三個部分: ( 1 ) axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建; ( 2 )探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理,並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響; ( 3 ) axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下: 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ,並將其構建入真核表達載體中,編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。The thymus hormone that the thymus excretes can contradict tumor, virus and bacteria, it can also improve the body ' s cell immunity, non - special immunity and combating infection ability through promoting cell ' s function and quantity
胸腺分泌的胸腺素具有抗腫瘤、抗病毒、抗細菌等作用,還能通過促進細胞的功能和數量的恢復,從而提高機體的細胞免疫和非特異型免疫,增強機體的抗感染能力。All these results suggested that the cause of 4d1 culture inhibiting the pathogenicity of scg1 is the decreased concentration of ahl molecule in scg1 cells, when presenting aii protein in 4d1 cells, and then not inducing the expression of virulence genes of scg 1 at low concentration of ahl molecules
由此推測,蘇雲金芽胞桿菌影響病原菌致病性的原因在於降低了病原菌細胞內ahl類信號分子的濃度,使其達不到激活毒素基因表達的水平。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。These anthraquinones are extremely cytotoxic ( they fight against ) towards a broad spectrum of bacteria, fungi, and viruses that include pneumonia, e coli, blood infections, diarrhea, skin infections and ( tuberculosis
蒽醌是一種極為有效的廣譜類抗細胞毒素物質,可抗擊多種細菌、真菌、病毒,包括導致肺炎的細菌和病毒、大腸埃希氏桿菌,以及導致血液感染、腹瀉、皮膚感染和肺結核的微生物。分享友人