膜電泳 的英文怎麼說
中文拼音 [módiànyǒng]
膜電泳
英文
membrane electrophoresis-
Electrophoreses identification of seed protein of radix glycyrrhizae and its adulterants
甘草種子及其混淆品的醋酸纖維薄膜電泳鑒定Cellulose acetate membrane automated electrophoresis systems
乙酸纖維素薄膜自動電泳系統This system contains : parts load, pre - degrease, degrease, spray cleaning, spray surface adjustment, spraying phosphating, rinse with d - ionized water, electro - deposition, ultra - filter for post rinse, oven curing, cooling and unload
由上件，預脫脂，脫脂，噴洗，噴淋表面調解，噴淋磷化，去離子水噴洗，電泳塗裝，超濾液噴洗，漆膜固化，自然冷卻，下件工序組成。二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system
二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳，利用tanongis凝膠圖像處理系統分析其含量。In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation
而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料，分別用高鈣溶液、生理溶液、低鈣溶液孵育后，通過sds ? page電泳技術及免疫膠體金電鏡技術，研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated
解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis
（ 2 ） cp細胞系在10低溫下細胞死亡率與時間成正比， 120d的細胞具典型的凋亡現象，即細胞核固縮、染色體邊集在核膜內側；細胞體積變小；瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。Using diethanolamine as aminating agent and glacial acetic acid as neutralizing agent, aminated epoxy acrylic cationic resin was prepared. the effect of technology of aminated epoxy acrylic resin on properties of eletrodeposition was studied by conductivity meter and electrophoresis apparatus. it was shown that, conductivity firstly decreased, and then increased with aminating temperature increase. in contrast with putting polyacrylic resin into thin acetic acid solution, the more compact film could be achieved by neutralizing polyacylic resin with glacial acetic acid and then add it into water. when neutralizing temperature was enhanced, the speed of electrodepsidon was found to increase, and the film was also more compact. increasing the dn leads to enhanced conductivity and smaller particle size. when dn equaled to 80, the smoothest film could be achieved
以二乙醇胺為胺化劑、冰醋酸為中和劑，合成了胺化環氧丙烯酸陽離子樹脂.採用電泳儀和電導率儀，研究了胺化環氧丙烯酸樹脂合成工藝對陰極電泳塗料電沉積性的影響.結果表明，隨著胺化溫度的增加，電泳液電導率先下降後上升.將冰醋酸加入樹脂中中和，後用水稀釋，比樹脂在醋酸稀溶液中中和，電沉積性能更好.電沉積速率隨著中和溫度的上升而增加，電沉積膜緻密性相應增加.中和度（ dn ）愈高，電泳液電導率愈大，粒徑越小，而塗膜外觀在中和度為80時達到最佳Dab served as chromagen. western blot thirty micrograms of protein extracted from untreated and bfgf, atra - induced mmscs cultures were separated on a 8 % gradient acrylamide gel and eletrophoretically transferred to a nitrocellulose membrane. the blot was probed for nse expression
4westernblot檢測誘導后細胞的nse表達情況從未經處理和經過bfgf ， atra誘導的細胞中提取30爬蛋白在8的sds一聚丙烯酸胺凝膠上電泳並轉移到硝酸纖維素膜上， 4 5脫脂奶粉封閉過夜。By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。Cellulose acetate electrophoresis apparatus
醋酸纖維薄膜電泳儀The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )
經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。The mechanisms of such treatment have been proposed as inhibition of proliferation and angiogenesis, as well as induction of differentiation and apoptosis, as has been tested by various in vivo and in vitro experiments. in our experiments, it has also been demonstrated that after the treatment of arsenic trioxide, the k562 cells has undergone major morphological changes, which included nuclear shrinkage, membrane bleb and scattered apoptotic bodies. dna gel electrophoresis also discovered that the typical " dna ladder " phenomena in the treatment group, while the control group showed the regular genomic banding
我們在實驗中觀察到as _ 2o _ 3作用人紅白血病k562細胞后，細胞生長明顯變緩，部分細胞出現皺縮、染色質濃聚及胞膜起泡現象，部分細胞胞膜破裂，在其周圍有緻密的凋亡小體出現， dna電泳出現典型的凋亡「梯狀」帶，提示as _ 2o _ 3能有效抑制k562細胞生長，誘導k562細胞凋亡。We also investigated the pathological changes of mouse liver, thymus and cerebrum cortex challenged by so2 inhalation by in vivo tests. we studied the apoptotic induction on mouse spleen cells and cytotoxicity of human embryo lung fibroblasts of so2 derivatives by in vitro tests. in vivo tests of sulfur dioxide inhalation showed : ( 1 ) effects on mouse lung of so2 challenge : we found no significant apoptotic changes induced by so2 inhalation but obvious pathological changes of lung with vacuolating of osmiophilic multilamellar bodies which maybe related with the decrease of surfacant and decrease of microvillus of type ii alveolar cells ; we also found thickening of part of basement lamina between type i alveolar cells and capillary endothelium cells which may inhibit the dispersion of oxygen and contribute to lung dysfunction
二氧化硫熏氣染毒的體內實驗結果表明，在本次實驗的濃度范圍內（ 56mg m ~ 3 、 112mg m ~ 3 、 168mg m ~ 3低、中、高三個濃度） ： （ 1 ）通過透射電鏡、 dna凝膠電泳分析和流式細胞分析發現二氧化硫吸入染毒一周對小鼠肺臟沒有明顯的凋亡誘導作用，但通過透射電鏡觀察發現二氧化硫可引起肺臟明顯的超微結構改變，引起型肺泡上皮細胞板層體空泡化，微絨毛減少，線粒體緻密化或腫脹變性；肺泡血管內皮細胞和型肺泡上皮細胞之間基膜增厚，使氧氣彌散功能出現障礙，從而降低肺功能。It also explains the surface finishing, such as gold plating, laminating and the surface protection methods of chemical process protection, electrophoresis lacquering, organic filming, etc
詳細敘述了銅牌、銅徽的光亮防護：鎏金、鍍金、烤金、鍍銀、貼金、假貼金、泥金、化學工藝防護、電泳漆、有機膜等。We did the same steps for three times, so we could get the extraction using the steps mentioned. laemmli sample buffer was added to the extracted protein, which were then boiled for 5 min. the protein samples were separated by 12 % sds - page and transferred to nitrocellulose membrane
樣品蛋白經12 % sds一聚丙烯酞胺凝膠電泳分離后，轉印到硝酸纖維素膜上，與第一抗體4孵育過夜，經tbs洗滌后，再與第二抗體室溫孵育2小時， ttbs充分沖洗后，顯色觀察。Ten isozymes and proteins were investigated by cellulose acetate membrane electrophoresis, the phylogenetic relationship among four groups of microtus fortis was analyzed based on the alleles frequency at these biochemical loci
應用乙酸纖維素膜電泳法分析了四類東方田鼠的10種同工酶及蛋白，並觀察了它們在這些生化基因位點上的基因分佈特徵以研究它們之間的遺傳關系。By gel filtration on sepharose cl - 6b and cellulose acetate pellicle electrophoresis test, hpa and hpb were respectively a single strait symmetry apex and the result of electrophoresis assume a single spectrum strip
經sepharosecl - 6b柱層析和醋酸纖維素薄膜電泳檢驗， hpa和hpb均為單一狹窄對稱峰且電泳呈單一譜帶。Isolate and determin the proteins of chicken serum by cellulose acetate membrane electrophoresis
醋酸纖維薄膜電泳法分離測定雞血清蛋白質分享友人