膠狀菌 的英文怎麼說
中文拼音 [jiāozhuàngjūn]
膠狀菌
英文
colloides-
Pay attention to personal hygiene. after you go to the toilet, wash your hands with soap and water to get rid of bacteria before you handle food. if you cut your hand, cover the wound with a water - proof plaster. if you have any symptoms of intestinal illness, such as diarrhea or vomiting, refrain from cooking
保持個人衛生，如廁后、處理食物前及煮食前應用肥皂和清水洗手，以免雙手沾上腸道細菌而污染食物；手上如有傷口，必須貼上防水膠布，以免食物沾上金黃色葡萄球菌；如有腹瀉及嘔吐等腸胃病徵狀，不應下廚。In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。Puried and identified by gel electrophoresis, the plasmid pbl29 is cccdna and the molecular weight of the plasmid pbl29 was estimated to be about 3. 7kb. the sensitivity of bacillus iicheniformis29 to 18 antibiotics was determined by inhibitory zone with filter paper
Licheniformis29菌株中抽提得到質粒pbl29 ，純化后通過瓊脂糖凝膠電泳觀察，確證質粒pbl29為閉合環狀質粒、分子量3 . 7kb左右。
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