膠質菌 的英文怎麼說
中文拼音 [jiāozhíjūn]
膠質菌
英文
jelly fungi-
Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da
採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱，通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰，這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的，通過tricine - sds - page檢測，只能檢測到一條分子量相對較大的細菌素，分子量在8 ， 570da左右。The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。The metabolites eliciting inhibition to foam cell formation process of macrophage produced by endophyte hccb00017 were studied. several products were isolated through solvent extraction, and silica gel chromatography et al. one compound, hccb00017 - a, showed cytotoxicity ; the other two, hccb00017 - c and hccb00017 - e, showed inhibitory activity against foam cell formation process of macrophage
對具有巨噬細胞泡沫化抑制活性的植物內生菌hccb00017的代謝產物進行研究，應用溶媒萃取、硅膠柱分離等方法，從其發酵液中分離出具有細胞毒性的活性物質hccb00017 - a ，以及具有巨噬細胞泡沫化抑制活性的組分hccb00017 - c和hccb00017 - e 。There are abundant bioactive substances, which can kill many kinds of microorganism. in order to verificated the antibacteria substances of chinese forest frogs, the skins of fresh forest frogs were drawn out by methanol solution 80 % and filtered with sephadexg - 100, sephadexg - 50 and sephadexg - 25
為了驗證中國林蛙皮膚中抗菌活性物質，以新鮮林蛙皮膚為實驗材料，用80甲醇溶液浸提，再經sephadexg - 100 、 sephadexg - 50和sephadexg - 25凝膠提純，獲得了純度較高的抗菌活性物質。Microbiology of food and animal feeding stuffs. horizontal method for the enumeration of coagulase - positive staphylococci staphylococcus aureus and other species. part 2 : technique using rabbit plasma fibrinogen agar
食品和動物飼料微生物學.凝膠水平計數方法.陽性葡萄球菌和其它屬.第2部分:兔原生質血纖蛋白質瓊脂培養基技術Eliminate water in the tank and harmful colloidal wax and mole in the tube
清除油箱內水份及油管內膠質、臘、酶菌等有害物。Reverse osmosis is in a salt water such as raw water than natural infiltration to exert greater pressure on the pressure and make the water from the high concentration side infiltrate low concentrations party to the original edema water pressure to the membrane elements on the other side into pure water and raw water minor impurities, colloid, organic matter, heavy metals, bacteria, viruses and other harmful substances and are all retained from the sewage discharge into export
反滲透就是在有鹽份的水中如原水施加比自然滲透壓力更大的壓力，使水由濃度高的一方滲透到濃度低的一方，把原水腫的水分子壓到膜的另一邊變成純凈水，而原水中的細微雜質膠體有機物重金屬細菌病毒及其他有害物質都統統截留下來並經污水出口排放掉。由於反滲透膜的孔徑僅0 . 0001Material of cardboard are environment friendly plastic plywood, no - poison, no - bacteria, belong to national product which free of inspection, no need drying and sterilize, material are free of fumigating with jasmine, and quality fully according with national commodity inspection bureau standards
本公司所訂做的卡板材質為世界環保型膠合板，無毒無細菌，屬國家名檢產品，無需烘乾和消毒處理，為免薰煙型材料，其質量完全符合國家商檢局要求The expression of lexa protein in the iptg - induced jm109 ( pza172 ) was checked by sds - page, and the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different concentrations of mmc
應用電穿孔技術將攜帶有抗輻射菌lexa基因的重組質粒pza172轉入大腸桿菌jm109 ，其啟動子為lacz ，用iptg誘導， sds - page凝膠電泳檢測lexa蛋白的表達。An antifungal protein, named as b16, was purified from the supernatant of the fermentation broth of strain 041381 by 80 % saturation ammonium sulfate, desalt and gel filtration on sephadex g - 75, which with molecular weight at about 30 - 40 kd on the basis of sds - page
菌株041381發酵上清液經70飽和度硫酸銨沉澱，透析脫鹽， sephadexg - 75凝膠過濾層柱，得到活性物質b16 。以sds - page膠為基礎進行電泳分析， b16的分子量為30 - 40kd 。To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully
本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5
將重組質粒轉化大腸桿菌dh5 ，於37誘導培養8h ， sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達，表達的雞ifn -融合蛋白分子量約為22ku 。The antimicrobial secretion could be divided into three sections, every section had antimicrobial activity. the first could be extracted by petroleum ether, the second could be extracted by ethyl alcohol, the third could dissove in water and could be separated by sephadex g - 50, g200 gel filtration chromatography and carbornmethyl cellulose - 1 anion - exchange chromatography and detected by a256, . the antimicrobial secretion had wide spectrum and had strong inhitory activity against germs and fungi, they could inhibit sixteen kinds of plant pathogenic germs, eight kinds of animal germs and eight kinds of plant pathogenic fungi
粗提物經石油醚萃取可得第一個活性部分，剩餘部分經無水乙醇萃取可得第二個活性部分，剩餘物質再經凝膠sephadexg - 50後有兩個峰，第二峰有活性，再經凝膠sephadexg - 200後有三峰，第二峰有活性，將其經過陽離子交換柱cm - 1後有兩峰，此兩峰均有抑菌活性。Bioremediation technology used to treat oil contaminated soil
放線菌對稠油污染土壤中膠質瀝青質的降解研究Bio - degradation of resin and asphalt in viscous - oil contaminated soil by actonomyces
稠油降解菌的篩選及其對膠質和瀝青質生物降解In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。Although, challenging work remains to determine the interfering substances ( e. g. particulates ) of different environments, distinguish the specific species with specific probe, and overcome the high detection limit of fcm ( 10 ( 4 ) - 10 ( 8 ) cells ml ( - 1 ) ), literature reports suggested that fcm / fl has a great potential for real - time monitoring of bioaerosols
雖然不同環境其介質之量測、建立特定菌種之特定引子及克服螢光顯微鏡之高偵測極限，均有其挑戰性，本文建議使用螢光染色配合螢光顯微鏡對生物性氣膠即時偵測有極大潛力。With bacterial cgc as main subject, the tests had been done to elucidate mechanism of self - organization for macroscopic rhythmic structure. the dynamics of cgc forming was observed by special techniques of waving culture and microscopic culture ; the differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning ; integrity of cell wall was examined under tem ; outer membrane protein was analysed by sds - page and various substance and factors for cgc formation were determined
採用特殊的波動培養和顯微培養技術觀察潛生體形成動態；應用原子力顯微鏡掃描，比較細菌潛生體與繁殖體在細胞壁外層結構和鞭毛數量的差別；用透射電鏡觀察細胞壁完整性，以十二烷基硫酸鈉?聚丙烯酰胺凝膠電泳分析外膜蛋白的改變，並通過實驗分析多種物質和因素對潛生體形成的影響。To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。Feature : super sonec independent spring with pressurized germ - proof cotton material. ensures quality sleep. space plant cotton : dissipates heat, elastic, environmental friendly and moisture and germ - proof
特點：超聲波獨立簧，並經防菌不織布壓裝而成，互不干擾，充分保證睡眠質量。進口太空乳膠綿：散熱透氣、回彈強、環保、防潮、防菌。分享友人