芐基青霉素 的英文怎麼說
中文拼音 [jīqīngméisù]
芐基青霉素
英文
benzylpenicillin-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Supply of 35 000 nos. of fluticasone propionate aqueous nasal spray to the hospital authority and the department of health as a 24 - month contract commencing 14 march 2006 supply of 5 510 000 benserazide and levodopa capsules tablets to the hospital authority and the department of health as a 24 - month contract commencing 30 march 2006 supply of 2 800 000 sertraline hydrochloride tablets to the hospital authority and the department of health as a 24 - month contract commencing 2 april 2006 supply of 117 000 000 paracetamol tablets to the hospital authority and the department of health as a 24 - month contract commencing 8 april 2006 supply of 270 000 vials of cefotaxime sodium for injection to the hospital authority and the department of health as a 24 - month contract commencing 22 may 2006 supply of 8 200 000 cloxacillin capsules to the hospital authority and the department of health as a 24 - month contract commencing 1 may 2006 supply of large saloon cars grade a to the government logistics department as a 12 - month contract from date of acceptance supply and delivery of 48 000 nos. of supermarket gift coupons to the electrical and mechanical services department as a 12 - month contract from date of acceptance
承投為醫院管理局和?生署供應35000個氟替卡松丙酸酯水劑鼻腔噴霧，合約由2006年3月14日開始，為期24個月為醫院管理局和?生署供應5510000粒羥芐絲及左旋多巴膠囊?藥片，合約由2006年3月30日開始，為期24個月為醫院管理局和?生署供應2800000粒鹽酸舍曲林藥片，合約由2006年4月2日開始，為期24個月為醫院管理局和?生署供應117000000粒對乙?氨基酚藥片，合約由2006年4月8日開始，為期24個月為醫院管理局和?生署供應270000瓶注射用頭孢?鈉，合約由2006年5月22日開始，為期24個月為醫院管理局和?生署供應8200000粒鄰氯青霉素膠囊，合約由2006年5月1日開始，為期24個月為政府物流服務署供應大型房車（甲級） ，合約由發出接納書日期開始，為期12個月為機電工程署供應和送運48000張超級市場禮券，合約由發出接納書日期開始，為期12個月Benzyl penicillin potassium
芐基青霉素鉀鹽Benzyl penicillin sodium
芐基青霉素鈉鹽2. the resistence of transformators were selected by g418 after co - culture with agrobactrium tumeflien, which was 40 mg / l in subcultured medium and 50mg / l in differeniated medium. in the process of killing agrobactrium, cef, amp or carb were also useful and the time of inhibition was long too
經與農桿菌共培養后轉化子的鑒定選用g418 ，在繼代篩選培養基中添加40mg l ，在分化篩選培養基中添加50mg l ；在除菌過程中，頭孢噻肟鈉、羧芐青霉素、氨芐青霉素抑菌效果較好，抑菌時間較長。First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a
用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。分享友人