In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋
芽莖段為外植體,研究了在四倍體刺槐高頻再生體系的建立過程中不同
基本培養
基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、
芽的分化及生根的影響;然後在得到高頻再生體系的
基礎上,通過農桿菌介導法轉化甜菜堿醛脫氫酶( badh )
基因,以gus染色組織分析為依據探討了影響轉化效率的各種因素,建立了高效、可重復的
基因轉化體系,為四倍體刺槐目的
基因的導入打下了
基礎。
Optimization of one isolate bacillus subtilis
一株枯草
芽孢桿菌發酵培養
基的優化
Usually the more basal and terminal nodes are less floriferous, or not at all.
通常越近
基部或頂端的節上花
芽越少,或根本沒有。
A small projection at the base of a basidiospore near its attachment to the sterigma
芽枝:位於擔孢子
基部連接擔孢子梗的一種小附屬物。
Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )
獲得轉
基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育,我們將3301 + 650和3301 + 651質粒用
基因槍和農桿菌介導法轉化煙草無菌發
芽的葉片,共207片(
基因槍109片,農桿菌98片) 。在含basta0 . 4mg l的篩選培養
基上進行篩選,得到抗性葉片181片(
基因槍93片,農桿菌88片) 。
Seed culture conditions of bacillus licheniformis ts - 01 were firstly investigated. the results showed that the optimal growth temperature was 40 c ; the suitable seed culture medium was beef extract soya peptone medium ; the optimal initial ph was 7. 5 ; the optimal seed culture time was 13h ~ 14h
首先對地衣
芽孢桿菌ts - 01的種子培養條件進行了研究,得到的結果為:最適生長溫度為40 ;較適合的種子培養
基為牛肉膏大豆蛋白腖培養
基;最適初始ph值為7 . 5 ;種齡為13h 14h 。
Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and 150mg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. leaves of adventitious shoots were detected by gus staining
Sin旮16ea )上暗培養3天後,移入加有抗生素150mg ikan和500mg幾cr的誘
芽培養
基上誘導不定
芽的分化。
Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution. positive shoots, gus tinted, were induced to root
分化出的不定
芽切下半張葉片進行gus染色,呈陽性的植株從外植體上切下,在生根培養
基( ms 0刀sing lnaa 50mg幾kan )中誘導根的形成。
Abstract : the anti - inflammatory effect of n - butanol extract of xanthocerassorbifolia bunge ( bex ) was studied in this paper. the ear edema resulted from dimethyl benzene, paw edema induced by carrageenin, the increase of vascular permeability caused by acetic acid, the chemotaxis of wbc induced by cmc and the weight of cotton granuloma in mice and hind paw edema induced by fresh egg white in rats were inhibited significantly by ig bex daily for 7 days, respectively. the carrageenin - induced paw edema was also inhibited markedly in adrenal - ectomiced mice. no changes in weight of adrenal and the concentration of vitamin c were observed, indicating that the anti - inflammatory effect of bex was not related to pituitary - adrenal system
文摘:文冠木正丁醇提取物對二甲苯致小鼠耳腫脹、蛋清致大鼠足腫脹、角叉菜膠致小鼠足腫脹、醋酸致小鼠腹腔毛細血管通透性增加、小鼠羧甲
基纖維素囊中白細胞遊走、小鼠棉球肉
芽腫生長均有顯著的抑制作用,同樣劑量下,文冠木正丁醇提取物對角叉菜膠致去雙側腎上腺小鼠足腫脹仍有顯著的抑制作用,且對小鼠腎上腺重量及腎上腺中維生素c的含量沒有明顯影響。
The cultural conditions such as temperature, fermentation period and the compound of medium are studied. the result of test show that suitable factors for both bacterium to grow and active substance to produce are 28, 200rpm and 72 hours. the bacterium is gotten through centrifuge with 8000rpm for 20min. then the bacteria is diluted and colophony named s - 8 is put into and used to absorbed active substance for 4 hours
對該菌株發酵條件的研究表明:該菌株用馬鈴薯葡萄糖液體培養
基發酵培養, 28 , 200rpm搖瓶中振蕩培養72h可獲得高活性的發酵產物,用蘇雲金
芽孢桿菌hd - 1做指示菌,將發酵液稀釋40倍生測仍可形成明顯的抑菌圈。
Wounded cotyledons of four - day - old seedlings of lettuce ( lactuca saliva l. )
外植體在無抗生素的誘
芽培養
基( ms 0
Floral organs have been successfully induced from the regenerated floral buds of ffyacinthus orienralis l. by precisely controlling exogenous hormones in the medium. under high concentration of cytokinin and auxin, the regenerated floral bud produces only tepals
以風信子花被片為外植體,通過控制培養基中細胞分裂素和生長素的濃度,可誘導花芽的再生和控制產生花器官的種類,建立起離體花器官定向控制系統。