芽突變 的英文怎麼說
中文拼音 [yátūbiàn]
芽突變
英文
bud sport-
The acrystalliferous and plasmid - free derivatives of bacillus thuringiensis were screened with ethidium bromide treatment, elevating growth temperature from 42 to 44 then to 46 by degrees, and treating it with 0. 05 % sodium docecyl sulphate ( sds ) as the plasmid - curing agent at 46
分別用溴化乙錠誘變處理、逐級升溫培養以及用sds處理等三種方法對蘇雲金芽胞桿菌無晶體（ cry ~ - ）和無質粒突變株進行了篩選研究。The recombinant plasmids pbmb121l, pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal, crylaclo and crylca from their original plasmid vectors to different plasmid vector. the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation. the transformation and expression properties of 8mb 171 were studied
通過將蘇雲金芽胞桿菌殺蟲晶體蛋白基因cry1aal 、 cry1ac10和cry1ca轉移至不同質粒載體上，分別構建了重組質粒pbmb121l 、 pbmb9821l和pbmb986l ，並分別導入無質粒突變株bmb171 ，篩選得到攜帶相應icps基因的重組菌株。4. effect of resolution vector on the expression of pesticidal crystal protein genes this work successfully introduced pesticidal crystal protein genes into crystal negative strain bmb171 by using resolution shuttle vectors. after resolution, the expression of cry1ac and cry 1c genes have no obvious change, but the expression of cry3a genes has great increase in the same condition
解離反應對殺蟲晶體蛋白基因表達的影響成功地利用解離載體將crylac10 ， crylc和cry3a基因導入蘇雲金芽胞桿菌無晶體突變株， crylac和crylc基因解離前後的表達量和殺蟲毒力未見明顯變化， cry3a基因在相同條件下則表達量有所提高，至於為何只對基因cry3a有作用尚不清楚，國內外也未見有人作相關報道。Effect of cry3a promoter on expression by using resolution vector, the cry1ac and cry1c genes with cry3a promoter or it itself promoter were introduced into b. thuringiensis crystal negative strains. the expression of these strains is quit similar, however, the expression of cry 1 ac and crylc with different promoter in same cell has no complete inhibition
Cry3a啟動子對基因表達的影響利用解離載體將帶cry3a啟動子的crylac10和crylc基因導入蘇雲金芽胞桿菌無晶體突變株，基因的表達和殺蟲活性與帶自身啟動子的基因的表達相近。The display of penicillin g acylase from bacillus megaterium not only offers the possibility of applying this technology for the selection of penicillin acylases with new side - chain specificities, but also facilitates our screen of mutant library constructed by using dna shuffling technique
這是首次在噬菌體表面展示出有活力的巨大芽孢桿菌青霉素g酰化酶，為利用噬菌體展示技術進行青霉素g酰化酶突變庫的篩選奠定了基礎。Kustaki : ori44, oriw30 and ori2062 were chosen to construct the gfp resolution vector. there were 9 kinds of gfp resolution vector. all of the recombinant plasmids were introduced into crystal negative b. thuringiensis 8mb 171. the fluorescence of the 9 kinds engineered strain can be detected by the fluorescent microscope
將得到的9種gfp解離載體電轉化到蘇雲金芽胞桿菌無晶體突變株bmb171中，熒光顯微鏡鏡檢顯示9株含gfp的蘇雲金芽胞桿菌重組菌在波長為300nm - 510nm的激發光下可發射綠色熒光。分享友人