The changing tendencies of the relative contents of phosphorous contained substances have been detected by in - vivo " p magnetic resonance spectroscopy ( in - vivo " p mrs ) during the whole hatching process. in - vivo ] p mrs proved the catabolism of adenosine 5 ' - triphosphate ( atp ), phosphorous ester and phosphocreatine ( pcr ) when the embryo dead. the results could be used to deduce the conversion of phosphorous contained metabolites during the chicken embryo developed
用活體核磁共振定域氫譜( in - vivohmagneticresonancespectroscopy , in - vivohmrs )對胚胎發育過程中羊水和蛋白、蛋黃的成分進行了分析;用活體磷譜( in - vivo 』 』 pmrs )的方法分析了在整個胚胎發育過程中含磷代謝物的相對含量隨時間的變
化,表明了磷脂類物質及三磷酸腺
苷( atp ) 、磷酸肌酸( pcr )在此過程中的變
化及可能的相互轉
化的趨勢,胚胎死亡后的磷譜也證明了磷脂類物質及三磷酸腺
苷( atp ) 、磷酸肌酸( pcr )在死亡過程中降解為無機磷的現象。
Chemotaxis mediated by chemokine receptors, such as cxcr4, play a key role in lymphocyte homing and hematopoiesis as well as in breast cancer metastasis. we have previously demonstrated that ? - arrestin2 functions to attenuate cxcr4 - meidated g protein activation and to enhance cxcr4 internalization. here we further show that expression of ( - arrestin2 in both hela and hek293 cells significantly enhanced the chemotactic efficacy of stromal - cell derived factor 1 ( ( sdf - 1 ( ), the specific agonist of cxcr4
- arrestin2是趨
化因子受體的一種重要的調節蛋白,本研究工作發現在hek - 293或hela細胞中升高- arrestin2的表達水平會顯著增強cxcr4介導的趨
化作用,反之當- arrestin2的表達被它的反義核
苷酸或rnai所抑制, cxcr4介導的趨
化作用則被明顯抑制。
In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核
苷酸序列設計一對pcr引物,以增殖的兩株iltv的dna為模板,分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上,轉
化感受態大腸桿菌,通過篩選對iltvtk基因的陽性克隆進行擴增培養。
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板,用甜瓜acc氧
化酶基因特異寡核
苷酸鏈為引物進行pcr擴增,得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上,篩選反向克隆,然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游,構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上,通過花粉管通道法轉
化河套蜜瓜,共獲76顆瓜,並進行了硬度和含糖量的分析。
Synthesis of tetraacetyl glucosyl esters and glucoside of coumarin
香豆素類葡萄糖酯與糖
苷化合物的合成與表徵
This charge is reduced by digestion with neuraminidase.
這一電荷被唾液酸
苷酶消
化而降低。
Synthesis and characterization of pyridazinone glucoside compounds
噠嗪酮類葡萄糖
苷化合物的合成與表徵
Effects of gstt on early atherosclerotic lesion of aortic intima in rabbits
蒺藜總皂
苷對家兔主動脈內膜粥樣硬
化病變的影響
Cyclic nucleotide dependent protein kinases
環
化核
苷酸依賴性蛋白質激酶
The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核
苷酸序列分析表明, pcr擴增產物中包含有完整的phya基因,該基因全長1506bp ,其中包含一段長102bp的內含子,該內含子具有真菌植酸酶基因內含子的特徵保守序列: donor序列? gtatgc , lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸,理論分子量為51 . 37kda ,其上有13個潛在的n -糖基
化位點, n端19個氨基酸為信號肽序列,植酸酶活性位點序列( crvtfaqvlsrhgaryptdskgk )位於氨基酸序列的+ 71 + 93 。
Udp - glucuronosyltransferases ( ugts ) are glycoproteins localized in the endoplasmic reticulum that catalyze the conjugation of a broad variety of endogenous and exogenous lipophilic aglycon substrates ( such as bilirubin, steroid hormone and drugs, insecticide, etc ) with glucuronic acid using udp - glucuronic acid ( udpga ) as the sugar donor. ugts are a gene superfamily of phase ii drug - metabolizing enzymes, they are responsible for the glucuronidation of a significant number of different functional groups ( e. g
Ugts能催
化各種各樣外源和內源的親脂性糖
苷配基底物與葡醛酸的結合,該反應是機體清除外源性(如藥物和殺蟲劑)和內源性(如膽紅素和甾體激素)親脂性
化合物的一個主要方式,也是藥物相代謝的重要方式。
The catalytic properties of solid superacid forthe synthesis of octyl polyglycosides
固體超強酸對合成辛基多
苷的催
化作用
Researchers can figure out the evolutionary relations of opsins in the various classes of cones and from different species by examining the sequences of nucleotide bases ( or dna “ letters ” ) in the genes that code for these proteins
透過比對視蛋白基因的核苷酸鹼基(或稱dna字母)序列,研究人員就能找出不同錐細胞視蛋白以及不同物種間視蛋白的演化關系。