草完隆 的英文怎麼說
中文拼音 [cǎowánlōng]
草完隆
英文
herban-
Contrasting analysis with the genes sequence of bacillus subtilis 168 strain ' s bio operon, the sequence of bio operon of as 1. 1094 strain had 12 bases difference with that of 168 train, and 7 bases caused variation of amino acid
1094菌株與168菌株bioafdb基因序列完全一致, 9個差異堿基是由於pcr擴增導致的。枯草桿菌生物素操縱子基因4個亞克隆序列的測序結果證實, asiIn order to use the responding ability to the inducers of pr - la promoter, two fragments, ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank. sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence. its transcription start site, tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter
根據pr - 1a基因的報道序列,設計兩對引物,以煙草基因組為模板,通過pcr擴增得到900bp ( ipl )和603bp ( ips )兩個目標片段,序列測定表明克隆的啟動子與報道序列具有99的同源性,轉錄起始位點、 tatabox及保守序列tgac與報道序列均完全相同。The predicted protein contained 441 amino acids. the activity of the epsp synthase encoded by these two subclones was shown by complementation of the aroa mutation in e. coli strain er2799, indicating two subclones possess a intact activity of the enzyme 5 - enolpyuvyl - 3 - phosphoshikimic acid synthase
採用互補試驗驗證分離的與草甘膦耐受相關的dna片段的生物學功能,結果顯示r3h1和r7h1均能使大腸桿菌epsp缺陷型菌株er2799恢復在限制性培養基上的生長能力,證明這兩個亞克隆的插入片段具有完整的epsp合成酶功能。The insert dna fragments are 7kb and llkb, respectively. two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e. coli jm109 up to 150mm glyphosate were constructed by subcloning the 2. 4kb and 3. 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript. sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase
以pgem - 3zf和pbluescript為載體,利用限制性內切酶hind和pst構建這兩個克隆的亞克隆,從中分別得到兩個草甘膦耐受亞克隆pgr3h1和pgr7h1 ,插入片段各為2 . 4kb和3 . 2kb ,對這兩個亞克隆進行序列分析,發現二者均含有一個核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ,其核苷酸序列長為1323bp ,推導的epsp合成酶由441個氨基酸組成,兩個亞克隆的草甘膦耐受濃度最大可達150mm 。分享友人