草黴素 的英文怎麼說
中文拼音 [cǎoméisù]
草黴素
英文
oxamycin-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers
，為了解決轉基因裙帶菜的篩選問題，進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗，包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素，除草劑類的basta ，氨甲喋呤類的氨甲喋呤，結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感，對basta非常敏感。A number of kanamycin resistant transformants were obtained two months later. they were morphologically normal, but grew slower comparing with the control tobacco
2個月後獲得了一批卡那黴素抗性煙草植株，抗性植株大田生長表型正常，生長速度相對緩慢。The test has adopted random control design, 1000 white feature boilers were randomly divided into 5 groups : basal diet + flavomycin, basal diet + 1. 0 % chinese herbal medicine, basal diet + 1. 5 % chinese herbal medicine, basal diet + 1. 0 % chinese herbal medicine + betaine, are basal diet
方法採用隨機單位組設計，將1000羽白羽肉仔雞隨機分為基礎日糧黃黴素、基礎日糧中草藥1 . 0 %水平、基礎日糧中草藥1 . 5 %水平、基礎日糧中草藥1 . 0 %水平甜菜堿、基礎日糧5組。3. through the ladder experiment of selecting transformer concentration by hyg, the proper selecting transformer concentration is 20mg / l hyg to tomato cotyledons and 35mg / l hyg to tobacco discs
經潮黴素梯度篩選試驗，最終確定番茄的適宜篩選濃度為20mg l ，煙草的適宜篩選濃度為35mg l ； 4Protective effects of bamboo leaves extracts on experimental myocardial infarct in dogs
人工蟲草提取物對慶大黴素誘導急性腎小管損傷的防治作用89 ) via agrobacterium mediation. pcr and southern blotting analysis showed that the cmo and badh genes were integrated into tobaccos " genome. betaine accumulation in transformed tobaccos was higher than that in control plants
89 ） ，獲得卡那黴素抗性和潮黴素抗性植株， pcr和southern雜交均證明外源cmo和badh基因大連理工大學博士學位已整合到煙草基因組中。In the second part of the thesis, we described that a tobacco chloroplast expression vector, ptrvp1, containing the foot and mouth disease virus ( fmdv ) vp1 gene and the selective marker aada gene was constructed and transfered to the tobacci chloroplast genome by the biolistic method
論文第二部分主要敘述了煙草葉綠體表達載體ptrvp1的構建，並通過基因槍方法轉化煙草葉綠體基因組，獲得了3株具有壯觀黴素抗性的轉化再生植株。Cbf1 gene was introduced into tobacco ( nicotiana tabacum ) by a plant expression vector pbi121 - cbfl containing the camv35s promoter using agrobacterium tumefaciens - mediated transformation. by pcr determination of cbf1 and kanamycin selection of transgenic progeny ( tl, t2 and t3 ), two pure transgenic lines were obtained. it was demon
經c萬盧7基因的pcr鑒定和轉基因後代（包括tl 、 tz和t3代）的卡那黴素抗性分析，獲得2個單一位點插入的轉基因純系，證明了外源基因在轉基因煙草中的表達及遺傳。The ced - 9 gene was transformed into leaves of tobacco by agrobacterium tumefaciens eha105. after twice selections of kanamycin cultures, 96 plants were regenerated from selective medium, and about 34 % of them turned out to be transgenic
然後以pbi121為對照組，通過根癌農桿菌eha105的介導，將ced - 9基因導入煙草葉片組織中，通過兩輪抗性篩選，得到抗卡那黴素（ km ）的再生植株。分享友人