莖段培養 的英文怎麼說
中文拼音 [jīngduànpéiyǎng]
莖段培養
英文
nod culture-
Minute fragments of shoot apices are proverbially difficult to culture.
微小的莖尖切段通常是難以培養的。In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。Studies on stems section tissue culture of rose chinese
月季莖段組織培養的研究Rate of cluster buds induced by different gingkgo explants is not the same. among them, cluster buds can be obtained directly from stems and mostly from axillar bud, but the amount is little and differentiation rate is only 20 %
銀杏不同外植體對叢生芽的誘導不同，銀杏莖段培養可以直接得到叢生芽，但數量極少，而且大部分屬于腋芽萌發，分化率20左右。Study on stem section culture conditions in vitro in ginkgo biloba
銀杏莖段試管培養條件篩選研究The different organs of elaeagnus mollis diels were used in this experiment as the explants. after establishing the aseptic propagation system, the aseptic tube stems and leaves were used to study the effect of different exogenous hormones combination and different culture conditions on organgenesis. the optimum medium and culture condition was screened for fast propagation of elaeagnus mollis diels
本實驗選材為胡頹子科胡頹子屬的翅果油樹，在建立起無菌繁殖系的基礎上，分別以試管無菌莖段和葉片為材料，研究了不同外源激素配比及不同培養條件對其器官分化的影響，從中篩選出適合翅果油樹快速繁殖的最適培養基和培養條件。3. potato stems and agrobacterium fumefaciens containing recombinant vector were co - cultured at 28cfor 48 hours and transplanted onto callus - inducing medium at 24c for 7 days. and then, the explants were transplanted onto differentiation medium and cultured at 24c for 21 days. resistant buds rooted and grew into plants in medium with kanamycin for 20 days, and 83 plants were obtained
3將含外源基因的根癌農桿菌與馬鈴薯莖段共培養后在愈傷誘導培養基上培養7天，轉接到分化培養基上分化出抗性芽，抗性芽在生根培養基上生根長成完整植株，共獲83株。Our experiment indicates : ( 1 ) the optimal concentration of kanamycin for screening torenia fournier regenerated buds was 400 mg / l. the ideal transformation was obtained in the following conditions : the leaf discs were dipped in agrobacterium suspension that od600 was 0. 1 for 10 ~ 20 min ; subsequently cocultivated on the ms solid coculture medium containing 20umol / l acetosyringon for 7 to 8 d at 23, and the induction ratio of regenerated buds was 27. 2 %
研究結果表明： （ 1 ）篩選藍豬耳轉化芽的最適卡那黴素濃度為400mg l 。 od _ （ 600 ）為0 . 1的菌液濃度菌液浸染葉盤10 20min ，固體共培養基（含20 mol l乙酰丁香酮）上23共培養7 8d可獲得理想的轉化效率，轉化芽誘導率為27 . 2 ； 16h光照， 8h黑暗是較理想的共培養光周期；莖段是較好的轉化受體。分享友人