菌受體蛋白 的英文怎麼說

中文拼音 [jūnshòudànbái]
菌受體蛋白 英文
bacterial receptor protein
  • : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
  • : 體構詞成分。
  • : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
  • : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
  • 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載中,再轉化大腸桿jm109感態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Now, in a study that took more than five years to complete, rockefeller university researchers, in collaboration with a team of bacteriologists at the university of wisconsin, madison, have become the first to solve the structure of a protein complex that protects these cells from singlet oxygen

    現在,洛克菲勒大學的研究人員與威斯康星大學的細學家一同協作,歷經5年多時間的研究,首次獲得了一種能夠保護細胞免單態氧損傷的復合的晶結構。
  3. They identified two bacteria, lactobacillus casei and lactobacillus plantarum, which can be added to beans so they cause minimal distress to those who eat them, and to those around the bean - lovers, marisela granito of simon bolivar university in caracas, venezuela and colleagues reported

    研究人員發現,把兩種細乳酸桿lactobacillus casei和植物乳酸桿lactobacillus plantarum添加到豆子中,就可以讓其產生氣的可能性變小。這樣豆子愛好者們和他們身邊的人就都能從中益。
  4. The pathogenic strains of yersinia enterocolitica carry a 45 - kb high pathogenicity island ( hpi ) comprising iron - uptake - related genes such as irp1 - irp2 and fyua, which has been observed in 93 % of the 60 entero - adhesive e. coli { eaec } strains and 80 % of the e. coli isolated from blood samples

    Hpi毒力島功能核心區主要的結構基因為irp2 、 irp1和fyua ,是高度保守的。 irp2和irp1分別編碼兩種高分子量hmwp2和hmwp1 , fyua編碼鼠疫
  5. Two main parts were included in the present thesis. part i. cloning and expression cryigenes of bacillus thuringiensis in different host strains

    一、蘇雲金芽胞桿殺蟲晶基因在不同中的表達1
  6. The recombination vectors were transformed into host strain of bl21 and induced with iptg. all the recombinant protein was expressed into inclusion body. the recombinant protein was identified with sds - page and westen - blot and purified through elution method. the muticlone antibody was got by immuning the rabbit with the purified protein

    把重組質粒轉化入表達bl21 ,經iptg誘導后都獲得了表達,且都以包涵的表達形式存在,用sds - page和western - blot的方法對重組進行了鑒定。
  7. Now, studies on immunogenicity of hp vaccine mainly focus on oral administration with liquid or microparticles. whereas the vaccine solution administrated orally would be digested and decomposed by the low ph and pepsin. though microparticle is a popular form, it has a series of significant problems such as low encapsulation rate and possibility of antigen denaturation as a consequence of expose to organic solvents and high temperature

    目前,國內外對幽門螺桿疫苗的研究僅局限在口服免疫,劑型以溶液或微球為主,眾所周知,液疫苗口服后易到胃內低ph及胃酶的破壞;微球雖然是疫苗載研究的熱點,但在制備過程中應用的有機溶劑及高溫操作會破壞疫苗的完整性,降低免疫效果
  8. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定,該基因為雞il - 2基因,其序列與sundick等報道的完全一致。在此基礎上,我們把雞il - 2基因亞克隆到大腸桿原核表達載pproex ~ ( tm ) ht中,構建重組表達質粒並進行確證性序列測定,重組質粒測序結果表明將編碼雞il - 2成熟的基因正確地插入到原核表達載pproex ~ ( tm ) ht的目的位點。重組質粒轉化dh5後用iptg於37進行誘導培養, sds - page和westernblot分析顯示,表達的雞il - 2融合分子量約為18kda ,表達的融合經薄層掃描發現目的表達量約占的30 。
  9. 2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin

    二、在以上研究的基礎上,對cmiv抗肽的c端進行較大的改造,即將與腫瘤細胞過度表達的表皮生長因子( egfr )具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗肽cmiv的c端,設計完整的基因,並在大腸桿中利用ptxfus表達載與硫氧還進行可溶性融合表達。
  10. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ,分別編碼501 , 333 , 183個氨基酸。其中orf1序列與hd263復制ori43的同源性為98 ,而另外兩個orf和genbank己公布的bt復制相關無同源性。 30連續培養72h ,復制區質粒在bt無晶突變株hd73cry ~ -中穩定性達98以上, 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳,對株無明顯不良影響。
  11. These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5

    方法在對hpk - 5 ( humanplasminogenkringle5 , hpk - 5 )因子的基因序列和質序列進行分析的基礎上,利用pcr技術分別構建其可溶性原核表達載和不溶性原核表達載;用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒,用不同的感態大腸桿( e
  12. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因,克隆到桿狀病毒表達載pfastbacl中,構建成重組轉座載pfb1 - cee ,轉化dh10bac大腸桿態細胞,篩選陽性落,抽提大分子質粒dna ,獲得含hcv結構區基因的重組桿狀病毒穿梭載bac - cee ,脂質介導轉染sf9昆蟲細胞,出現細胞病變后,收集含有重組桿狀病毒顆粒的培養上消,重新感染sf9細胞,收集sf9細胞,進行12 . 5 sds -聚丙烯酰胺凝膠電泳,可見表達的條帶。
  13. Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle, immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens, the transformation rate of the above acceptor was investigated respectively. the results showed that immature embryo after precultured for 4 ~ 6d was the best. in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb, to agrobacterium tumefaciens eha 105, od value of 0. 8 was the best

    採用農桿介導法將bt毒基因導入水稻同樣以上述兩種秈稻為主要研究材料,比較了分別以預培養的幼胚和幼穗、幼胚、成熟胚來源的愈傷組織作為轉化的愈傷組織轉化頻率,結果表明預培養4 6天的幼胚最適宜作為農桿介導轉化的;其次是來源於幼胚和成熟胚的生長狀態良好的胚性愈傷組織。
  14. Sds - page proved that the molecular weight of expressed product of the extracellular part of bt - r3 was about 160kda. western blotting showed that the recombinant protein could specifically bind to bt toxic protein crylab, indicating that the bt - r3 is a receptor protein of crylab. the binding site of bt - r3 with crylab was located in extracellular domains of bt - r3 protein

    用大腸桿系統表達的bt - r3基因的胞外結構域的分子量約為160kda , western - blot實驗證實,重組能有效地結合bt毒cry1ab ,從而進一步確證了bt - r3是cry1ab的基因。
  15. The recombinant plasimid ppic9k - pzp3 a - hcg p - ctp109 - 145 was transformed into electroporated pichia pastoris and screened the positive strains on md plates without histidine. multi - copy recombinants were screened on the g418 - ypd plates and then were incubated with the supernatant containing methanol. the recombinant pzp3 a - hcg p - ctp 109 - 145 protein were secreted in the supern atant and were verified

    轉化嗜甲醇酵母獲得耐4mg mlg418的株,經sds - page電泳和westernblotting檢測表明其上清培養液中的可與猴抗pzp3抗和兔抗hcg抗發生特異性的反應。
  16. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載上,並轉化e colidh5a感態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載並轉化大腸桿dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合,並能被口蹄疫病毒陽性血清識別。
  17. Gfp can be expressed in the host enterococcus facecalis and lactobacillus plantarum we isolated using the expression vectors we constructed the expression level depends on the nisin concentration to some extent

    Ow洲poi114no例烴們表達載在我們分離到的efqecalis和l plantarum中能夠表達綠色熒光oppx表達的水平依賴於一定范圍濃度的nistn誘導。
  18. At first, the vrl - cdna plasmids were transformed into tg1 r. coli to enlarge vrl - cdna ; then the plasmids were extracted and cut off with enzymes ; subsequently, vrl - cdnas were transcribed into vrl - inrna by t7 or sp6 polymerase in vitro, following vrl - mrnas were injected into xenopus laevis oocyte to express into vr1 receptor protein ; at the end the vrl + - oocytes were tested by double electode voltage clamp

    這項實驗中,我們首先將大鼠vr1 - cdna質粒轉入大腸桿中進行擴增,然後提取質粒並酶切,然後在外轉錄成vr1 - mrna ,接著將vr1 - mrna注射到非洲爪蟾卵母細胞中表達為,建立了實驗模型細胞,最後用雙微電極電壓鉗檢測此模型細胞。
  19. In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg

    同時將原核表達載pet - 28c用nco , xho雙酶切,回收酶切產物,將回收的酶切產物pea , h3 ,載進行連接,並轉入dh5感態細胞內,培養12 - 18小時后,挑取陽性落,經nco , xho雙酶切分析及pcr檢測,篩選到陽性克隆,其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組h3融合基因的原核表達載
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