菌種活化 的英文怎麼說
中文拼音 [jūnzhǒnghuóhuà]
菌種活化
英文
actication of culture-
These bacteria are transmitted through the cytoplasm of eggs and have evolved various mechanisms for manipulating reproduction of their hosts, including cytoplasimic incompatibility ( ci ), parthenogenesis, feminization, male killing, fecundity or fertility modifying etc. wolbachia are common and widespread bacteria infecting 17 % insect population, 17 crustacean species, mites and many nematodes
這些共生菌通過母系遺傳參與調控寄主的多種生殖活動,包括細胞質不親和現象、誘導孤雌生殖現象、雌性化、雄性致死以及調節寄主生殖力等。 wolbachia的分佈十分廣泛,目前已經發現在超過17的昆蟲種群以及17種甲殼綱動物、蟎蟲、線蟲體內發現了它的存在。In the stomach, this acid functions to kill bacteria in foods, to soften foods and to convert the inactive enzyme pepsinate into its active from pepsin, to begin the digestion of protein
在胃裡,這種酸的作用是殺司食品的細菌,使食物軟化,把鈍化的胃蛋白酶原轉化為有活性的能消化蛋白質的胃蛋白酶。For enhancing the soakage capability and the combine intension between inorganic films and stainless steel plate, and improving the reactivity of stainless steel, to enhance the combine intension between sca film and stainless steel plate, the stainless steel plates are oxidized. the experiment prepared two oxidized stainless steels in different oxidation degree, aa and ab, respectively used as experiment plates in silver doped tio2 or sio2 antibacterial stainless steel and silver doped sca antibacterial stainless steel
為提高無機薄膜和不銹鋼基板之間的浸潤性和結合強度,以及為使不銹鋼基板具有更高反應活性,以提高有機硅烷薄膜與不銹鋼基板間的結合能力,對不銹鋼基板進行氧化處理,制備兩種氧化程度不同的不銹鋼: aa和ab ,分別作為摻銀tio _ 2 、 sio _ 2抗菌不銹鋼和摻銀有機硅烷抗菌不銹鋼的基板材料。The results indicated that the department had complied with the requirements of four key elements indicating the quality of indoor air as recommended by the environmental protection department, namely the intensity of carbon dioxide, air - borne dust particulate, radon and total viable count
本局委託了機電工程署就本局位於稅務大樓的辦公室進行室內空氣質素測量,結果顯示大樓的室內空氣質素,符合環境保護署建議的四個空氣質素主要成分的規定。這四種含量分別是二氧化碳、空氣微塵粒子、氡和總活菌素。In this study a microbial system for biphenyl biodegradation is set up in order to investigate the effects of surfactants on biodegradation of hydrophobic organic compounds. four strains which can grow on biphenyl as the sole carbon and energy sources are selected out, in which alcaligenes eutrophus dj1 and pseudomonas sms02 are chosen as degradation strains. after studing the bioavailability of three nonionic surfactants ( op - 10, tween - 80, and triton x - 100 ), they are added into the biodegradation system of biphenyl
為了考察表面活性劑對疏水性有機污染物生物降解的影響,本論文選擇聯苯作為模擬研究體系,篩選、馴化得到四株可利用聯苯作為碳源生長的菌株;考察了聯苯降解菌株對三種非離子表面活性劑op - 10 、 tween - 80 、 tritonx - 100的生物可利用性;用高效液相色譜法測定了非離子表面活性劑對聯苯的增溶曲線;較系統地研究了加入非離子表面活性劑對聯苯生物降解速率的影響。Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究:無氮培養條件下,測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響,結果表明,在15 - 40下均能生長並表達固氮酶活性,其最適生長及固氮的溫度為25 - 35 ;能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮,當培養基中同時存在蔗糖和蘋果酸時,細菌生長和固氮活性最強;在偏酸和偏堿的條件下( ph4 . 5 - 8 . 5 )均能保持較強的生長勢和較高的固氮酶活性,並能通過調節自身代謝平衡並適應環境的酸、堿性變化,使培養液趨于中性:能耐受較高的滲透壓,培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大,當naci濃度升至2時,菌株的生長勢及固氮酶活性才有所下降:低濃度的鉸對其固氮酶活性影響不大,在0The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現:三種酶的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ; xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶的比活2814 . 45iu mg ; xynb和xynba的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb的km值較大為27 . 10 ( g kg ) ; xynba和xynbb的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好的抗性,且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。Bacillus, denitrifying bacteria, denitrifying bacteria, single spore, photosynthetic bacteria and various beneficial living bacterium, the lively effective bacterium number are not less than 2 billion / grams
芽孢桿菌、硝化菌、反硝化菌、單孢、光合菌、硫化菌等多種有益活菌,有效活菌數不低於20億/克。Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on
該蛋白質中含有保守的- cp ( g ) pc -氨基酸活性基序,該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞,對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連, trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。In the study, four models were applied and used for prescreening antimicrobial, antitumor and the a - glucosidase inhibitory agents from medicinal fungi including filamentous fungi and macro fungi in fujian ; we studied the antimicrobial, antitumor and a - glucosidase inhibitory activities of three kinds of ganoderma sp. ; as well as we studied the a - glucosidase inhibitor from filamentous fungi w - 20 and its physical and chemical properties. there are abundant antimicrobial, antitumor and a - glucosidase inhibitory agents in filamentous fungi and macrofungi
本課題以福建省的藥用真菌資源為對象,應用了抗菌、抗腫瘤和-葡萄糖苷酶抑制劑的體外篩選模型,進行了藥(食)用曲的絲狀真菌和大型真菌生物活性物質的篩選;對3種靈芝真菌的抗菌抗腫瘤、 -葡萄糖苷酶抑制活性,以及絲狀真菌w - 20抑制-葡萄糖苷酶的活性物質及其理化性質進行了初步研究,為我省藥用資源的開發利用打基礎。The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )
經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析,獲得一條約120kda的表達條帶; iptg誘導表達后提取原生質膜測定透明質酸分解酶活力,表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。It permanent release negative ions, firing far infrared, wipe out indoor formaldehyde, benzene, ammonia and other harmful gases, deodorant antibacterial, purifying water and air, activated human cells, and promote blood circulation, eliminate fatigue, improve sleep, improve human immunity and other functions. it can be used in a very wide range
它具有永久性釋放負離子、發射遠紅外線、祛除室內甲醛、苯、氨氣等有害氣體、除臭抑菌、凈化水和空氣、活化人體細胞、促進血液循環、消除疲勞,改善睡眠,提高人體免疫力等多種功能,用途范圍十分廣泛。It has permanent release negative ions, and far - infrared emission, deodorant antibacterial, purifying water and air, activated human cells, and promote blood circulation, eliminate fatigue, improve sleep, improve human immunity, prevention of heart diseases, cervical spondylosis, hemorrhoids, and other health - care functions
它具有永久性釋放負離子、發射遠紅外、除臭抑菌、凈化水和空氣、活化人體細胞、促進血液循環、消除疲勞、改善睡眠、提高人體免疫力、預防心臟病、頸椎病、痔瘡等多種醫療保健功能。The protective efficacy on lactobacillus casei shirota of four cryoprotectants, including skim milk, glucose, sodium glutamate and fructose, was studied by viable cell count and lactate dehydrogenase activity analysis of rehydrated starter made by freeze - drying l. casei shirota
摘要將活化后的乾酪乳桿菌代田株製成凍干發酵劑,通過對其復水后活菌數和乳酸脫氫酶的測定,比較了脫脂奶、葡萄糖、谷氨酸鈉、海藻糖4種凍干保護劑對乾酪乳桿菌代田株的保護效果。The main effect factors include : 1. microbial species, which seem to have different degradation rate under varied circumstances and n - alkanes ; 2. physical and chemical properties of crude oil itself, which have an important effect on biodegradation, such as conglutination, boiling point, refractive index, ingredient and concentration of the oil ; and 3. culture condition of microorganism, under which the degrading activity of microorganism will be affected by surfactant, light condition, sorbent, nutrition, co - metabolite, oxygen, temperature and salinity during the inoculation of selected or indigenous microorganisms
其中主要影響因素包括:菌種的影響,菌種在不同的環境中和對不同碳鏈長度的碳氫化合物表現出不同的降解效率;石油物質本身物理化學特性的影響,如石油物質在水體或土壤中的濃度以及石油的粘度、沸點、折射率等特性;生存環境條件的影響,在接種入高效率的降解菌或利用土著微生物進行降解時,降解率受到生存環境中各種條件的影響,如表面活性劑、光照條件、吸附劑的利用、營養鹽、共代謝底物、氧氣、溫度、鹽度等。Oligochitosan is not only water - soluble, non - toxic, biocompatible but also possesses versatile functional properties of chitin and its derivatives, such as polyelectrolite properties, the presence of reactive functional groups, gel - forming ability, high adsorption capacity, bacteriostatic, fungistatic and antitumour influence, and thus it has attracted more and more attention in the field of biology. previous investigations have shown that chitin and its derivatives have immunoenhancement activities
殼寡糖是由3 10個n -乙酰氨基葡萄糖殘基或氨基葡萄糖殘基通過- 1 , 4 -糖苷鍵連接而成的寡糖,它具有良好的水溶性、組織兼容性,在生物體內易降解,同時也具有抑菌、抗腫瘤、調血脂、調節免疫及活化腸道雙歧桿菌等多種生理功能,因此已成為眾多領域的研究熱點。The results proved the feasibility of this enrichment technique. the enriched nitrifying bacteria can directly store in a lucifugal condition for a long time, the nitrifiers decay at a rate of 0. 0138d - 1. but the decay rate of the nitrifiers stored in phosphate buffer ( ph 7. 2 ) is 0. 023d - 1
硝化細菌富集培養后直接避光保存是一種較佳的保存方式,經3個月的保存其硝化細菌存活率為26 . 7 ,其比衰減率為0 . 0138d ~ ( - 1 )硝化細菌。When bacteria enter the detector, their normal metabolism cleaves an inactive nutrient into an active one, allowing the detector ' s spores to germinate
當細菌一進入偵測器后,細菌的正常代謝反應就會將一種不活化的營養物質,裂解成有活性的東西,促使偵測器內的孢子開始生長。It is a famous and costly medicinal fungus, and it was investigated clearly in its biological feature and chemical components. now cordyceps sinesis ( berk. ) sacc can be produced by unnatural cultivation, not native collection, which brings many advantages of its output and application
本課題以提高冬蟲夏草菌絲體的主要活性化學成分腺苷的含量為目的,對冬蟲夏草cordycepssinensis ( berk . ) sacc .菌株cs - js進行了菌種選育和發酵的研究,並初步建立了冬蟲夏草發酵菌絲體的毛細管電泳指紋圖譜,為冬蟲夏草菌絲體產品的研發和產品的鑒別打下了基礎。In order to get some functional clues from their structures, the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors, their functions include regulating tissue - specific gene expression, regulating expression of genes related to growth and early development of cells, besides this, regulating expression of genes under some stimulated conditions, and so on. predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family, and there are high similarity between ndrg2 and epoxide hydrolase from bacteria, this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress, maintaining the balance of cell redox potential, involving in the metabolism process of xenobiotics or intracellular toxic molecules
研究發現呷基因的調控區存在多種轉錄因子結合位點,功能主要涉及組織特異性表達調控,細胞生長發育相關基因的表達調控,刺激反應基因的表達調控等; ndrgz蛋白在結構上屬于a小水解酶類折疊,折疊分類預測表明ndrg2與其中的的細菌環氧化物水解酶的二級結構極為相似,提示ndrgz蛋白具有一定酶活性,可能參與細胞抗氧化應激反應,維持細, an ) armtbffiofbfochmilsyn ) mdafblechmrbfobo4第四軍醫大學碩士學位論文胞內氧還電勢平衡,參與內外源有毒物質的代謝等。分享友人