菌索基 的英文怎麼說
中文拼音 [jūnsuǒjī]
菌索基
英文
hapteron-
Magnetic gradiometer sensors based on the amr sensors are build up and by which the experiment of magnetic anomaly field of ferrous targets are made which show the characters through the way of figure. it give the model of searching sensors imitating the way that bacteria look for the food and then the searching sensors for the ferrous targets are made up, which are also based on the hmc1022 - amr sensors. then the research of the searching and the results was given
提出了模擬細菌覓食行為的目標搜索傳感器模型,並以此模型為基礎利用amr傳感器hmc1022開發了應用在磁性目標搜索中的?型傳感器,然後以此傳感器為基礎對磁性目標搜索過程進行了研究。論文的最後給出了全文的結論,對磁異信號目標探測技術的實驗進行了總結分析,提出了研究中存在的問題,為進一步深入研究奠定了基礎。5. exploring fermentation synthesis of quinic acid ( qa ) using bioengineering strain under shake flask conditions synthesis of qa using recombinant e. coli constructs was examined in m9 accumulation medium. the result indicated that qa was produced from d - glucose in the culture supernatant, but it is necessary to explore further the consequences of fermentation in next work
工程菌搖瓶發酵產酸初步觀察初步探索了奎尼酸工程菌在m9積累培養基中的發酵產酸條件,結果表明工程菌經直接發酵由葡萄糖產生了奎尼酸,但尚須進一步優化發酵條件提高產酸率。A database search revealed that the putative sequence of the red gene shows 40 - 50 % identity with those of uroporphyrinogen iii methyltransferase ( encoded by coba gene ) from various kinds of bacteria. an over - expression of the coba gene in e. coli was reported to lead to an accumulation of trimethylated derivative of porphyrin termed trimethylpyrrocorphin and factor ii, which emit strong red fluorescence under uv
在ddbj中搜索到多種細菌來源的coba基因(編碼uroporph仰nogenhmethyltransferase )與redsene有40 50的同源性,並據報道,其中一個來源於pmpboibaclerilllaslldelll切chit的coba的基因,轉人大腸桿菌、酵母菌及動物細胞后能使表達載體在紫外線下發射紅色熒光。The present study was aimed to develop a solid - culture - based technology for easy and cheap propagation of p. delphacis f95129 inocula using small grains as substrate for fungal growth. sporulation capacity and timing pattern were relied upon to evaluate culture quality and identify potential factors to affect the quality. infectivity or virulence of inocula derived from the culture was assayed against the green peach aphid, myzus persicae ( sulzer ), based on time - dose - mortality modeling
針對這一普遍性的技術難題,本研究以飛虱蟲癘霉f95129菌株為對象,以黍米、粟米為基本材料,探索建立簡便易行的侵染體繁殖技術體系,並對培養物進行了產孢潛能及其影響因子的評價以及針對桃蚜( myzuspersicae ( sulzer ) )的侵染性測定和時間?劑量?死亡率模型模擬。The symbiosis between mesorhizobium huakuii and astragalus sinicus is a chinese - characteristic symbiotic nitrogen fixation system, while molecular genetic study on its early symbiotic interaction is still at primary stage
本文對新型報告基因?綠色熒光蛋白基因在華癸中生根瘤菌-紫雲英共生固氮體系早期結瘤階段分子遺傳學中的應用進行了探索性研究。Nucleic acid sequences of azoreduclase were searched and blasted in genbank. a pair of primers based on the conserved regions were designed. a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced. the sequence contained a complete 471bp orf ( open reading frame )
脫色實驗證明沼澤紅假單胞菌( rhodopsedomonaspalustris )對偶氮染料有較強的降解能力,我們通過genbank搜索,對所獲得的所有偶氮還原酶基因在ncbi進行比對並設計引物,從沼澤紅假單胞菌質粒中擴增獲得了一條含471bp完整開放閱讀框架的序列。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性,設計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當的限制性內切酶位點插入表達載體pbv220 ,分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆;進一步通過pcr技術構建echistatin的融合表達基因克隆。Different cultivation condition such as induced time, methanol concentration and ph of culture medium was studied at shake flask cultivation
通過搖瓶培養初步摸索了誘導時間、甲醇濃度、培養基ph值等培養條件對mut ~ +重組菌株表達pap的影響。Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein
本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒,在大腸桿菌中實現融合蛋白的可溶及分泌表達,同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ,為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。Recently, a new gene dr0167 ( pprl or irre ) that serves as a general switch for downstream dna repair and protection pathways via its regulatory function on the gene expression of reca, ppra was discoveried. expression of d. radiodurans pprl also promotes dna repair and protection pathways and enhances the radioresistance of e. coli. this finding provides a new clue to understand the mechanism of dna repair, especially double strand break ( dsb ) repair
最近我們實驗室在耐輻射球菌電離輻射敏感株中鑒定了一個與電離輻射抗性相關的基因ppri ,該基因可能通過調控dr細菌reca 、 ppra等基因的表達加速對電離輻射引起的dna損傷修復,而在大腸桿菌中表達ppri基因能促進reca 、 soda等表達水平顯著提高,使其抗輻射和抗氧化能力明顯增強,這將為我們理解其特殊抗性機制,特別是雙鏈斷裂修復提供新的線索。Bcc algorithm much improves the performance of bc algorithm while possessing the searching ability of a single bacterium, is a kind of potentially powerful optimization method worth of much more research
細菌群體趨藥性演算法在保留單個細菌較強的搜索能力的基礎上克服了細菌趨藥性演算法收斂速度較慢的不足,是一種具有進一步研究價值的新型函數優化方法。To date there is no specific database for toxin and anti - nutrient proteins. in order to establish such a database, we have collected data from some nucleotide and protein database available at present. totally, 1033 toxin proteins, including 172 from plants, 251 from animals, 577 from bacteria and 42 from " other organisms, as well as 1013 lectins and 391 proteinase inhibitors are collected
本文通過對主要基因或蛋白數據庫進行檢索,收集散落於不同基因或蛋白數據庫中的毒蛋白氨基酸序列數據1033個,其中植物毒蛋白172個,動物毒蛋白251個,細菌毒蛋白557個,其它生物如真菌、藻類等的毒蛋白42個;抗營養因子蛋白數據1404個,其中凝集素1013個,蛋白酶抑制劑391個。Make all these together, it proved that the cloned gene represented the major outer membrane protein gene of ahl316, and the expressed gene products shared identical antigenicity with the natural main outer membrane protein. the studies on preparation and application of momp - iscoms of ah l316 provided a new approach to fish vaccinology. the successfully cloning and expressing the major outer membrane protein gene of ah l316 made it possible to describe this gene ' s function under a single factor level, and also provided technical support for developing an advanced gene engineering vaccine and subunit vaccine against aeromonas hydrophila
鰻源嗜水氣單胞菌l316主要外膜蛋白免疫刺激復合物的制備與應用研究,對研製魚類疫苗學問題進行了新的初步探索;成功地克隆和表達嗜水氣單胞菌l316主要外膜蛋白基因為在單因子水平上研究嗜水氣單胞菌外膜蛋白的作用和免疫功能以及制備嗜水氣單胞菌基因工程疫苗和亞單位疫苗奠定技術基礎。The lactase gene from kluyveromyces lactis was researched in the thesis. the cloned gene was expressed in e. coli and the properties of lactase was determinated. in addition, we studied the expression of lactase gene in the methylotrophic yeast pichia pastoris
本論文從一株乳酸克魯維斯酵母菌中克隆獲得乳糖酶基因,在大腸桿菌中進行表達並測定其酶學性質,同時也對利用巴斯德畢赤酵母( pichiapastoris )系統表達該基因進行了探索。The expression protein products as insoluble inclusion bodies accounted for 15 % and 30 % of whole bacterial protein. two methods, b - per reagent only and triton - urine with sonication lysis of cells, were both access to the relatively good washing effect
通過摸索iptg誘導濃度,誘導溫度,菌體收獲時間等條件,確定了eo基因能在pgex4t bl21 ( de3 ) codonplus和pmal - p2x bl21 ( de3 ) codonplus中高效表達,表達產量分別占菌體總蛋白的15和30 ,表達的重組蛋白主要為包涵體。Without documents related to original strain, this thesis had to probe into the primary technological conditions of original strain. and eventually made the certain radial fermentation condition
在未提供出發菌原始資料的情況下,對出發菌的斜面培養基及搖瓶配方、生長習性等進行了摸索,確立了基本的發酵條件。Compared with minimal medium, rich medium leads to higher expression level up to 4 mg target protein / l. the optimal culture conditions we - e obtained as follow5 : complex medium buffered to ph 6. 0 ; harvesting cells after 36 hours of methanol induction
對菌體培養條件進行的探索表明:蛋白表達的最佳ph值應為6刀,最佳誘導時問為36小時,培養基為營養豐富的復合培養基。分享友人