菌落形成 的英文怎麼說

中文拼音 [jūnxíngchéng]
菌落形成 英文
colony formation of micro-organism
  • : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
  • : 落名詞[方言] (北方對蓮花落的俗稱) a kind of folk song
  • : Ⅰ動詞1 (完成; 成功) accomplish; succeed 2 (成為; 變為) become; turn into 3 (成全) help comp...
  1. The virulent strains had a polysaccharide capsule and formed smooth colonies on agar medium.

    有毒株具有多糖莢膜它在瓊脂培養基上光滑的
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中,轉化大腸桿dh5株,篩選氨芐青霉素抗性,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。
  3. 3. the effect of sporulation - independent promotor on toxicity of natural strain in order to study the effect of sporulation - independent promotor ( p3a ), p3a was spliced with the cry1c gene, then inserted into the shuttle vector pht304, and then recombinated plasmid pbmb827 was obtained. after transferring pbmb827 into strain ybt - 1520, it was surprising that the transformants had almost no potency against all lepidopteran larvae tested

    3非依賴芽胞icp的cry3a啟動子( p _ ( 3a ) )對野生株特性的影響帶p _ ( 3a )和cry1c基因的重組質粒pbmb827轉入ybt - 1520 ,轉化子對所測昆蟲的毒力下降非常明顯,芽胞和晶體也很難脫
  4. An extensive program of chemical modificaton can also be carried out to discover more active avermectins

    這種有白點的態與灰色類似,可能為放線的異核體。
  5. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ,反轉錄cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因,並通過? dna連接子將二者連接起來mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態tg1大腸桿,制備細式的mg _ 7重組噬體抗體庫;通過計數和限制性酶切分析( ecor和hind )評估mg _ 7重組噬體抗體庫的容量和重組率。
  6. The morphological, physiological, biochemical and genetic diversities between bacillus thuringiensis wild - type strain ybt - 1463 and its plasmid - free mutant bmb171 was comparatively studied. it showed that the plasmid - free mutant strain bmb171 lost the ability to form the parasporal crystal, but there was on obvious diversities were observed on the sensitivity to 10 antibiotics, the utilization of 19 carbon sources and 12 nitrogen sources, as well as the growth properties between ybt - 1463 and bmb171, whereas the electro - transformation frequencies of bmb171 were much higher than those of ybt - 1463, respectively with 5 exogenous plasmids as the donor dnas

    對出發株ybt - 1463和其無質粒突變株bmb171的部分態、生理生化和遺傳學特性進行的比較研究的結果表明,突變株bmb171不伴胞晶體,但在個體態與特徵、對紅黴素等10種抗生素的敏感性、對葡萄糖等19種碳源和谷氨酸等12種氮源的利用能力及生長性能與出發株ybt - 1463無明顯差異。
  7. First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d

    首先在對兩親株的生物學特性進行了鑒定后,考察了影響兩親株原生質體和再生的主要因素,確定了生米卡鏈黴和紅黴素鏈黴原生質體及再生的最佳條件:前者用s培養基,在28 、 220r . min ~ ( - 1 )培養24h后,用3mg ml的溶酶在32恆溫酶解50 60min ,得到的原生質體在乾燥的r5培養基上28倒置培養5 6天,可得到再生率在20左右的再生;後者採用二級絲培養,用1mg ml的溶酶在37恆溫酶解1h左右,得到的原生質體也在乾燥的r5培養基上28倒置培養5天,即可得到再生率在20左右的再生
  8. They incorporate chemical compounds that, following inoculation and incubation, produce a characteristic change in the appearance of bacterial growth and / or the medium surrounding the colonies, which permits differentiation

    它們納入化合物,因此在接種與培養后細生長的外觀或者在的四周一種具有特色的改變,這種改變具有分辨能力。
  9. The character of the colonies is important for special species. the results show that the characters of conidia shape and color are very important, the steady number of distosepta and the size of conidia are important too. otherwise, the special hosts corresponding to the species help us identify these species

    以分生抱子的態特徵為主,特證僅對特殊的種有參考價值:以分生袍于的假隔膜數的差異分大的類群,分生泡于的顏色、狀是主要的分類依據:狀相似時,長、寬作為區分的參考;此外,有些種具穩定的寄主,寄主也可作為鑒定的重要參考依據。
  10. Bacteria at the epd laboratory within six hours of collection. the membrane filtration method involves passing the water through a membrane filter, which retains bacteria in the water. the membrane is then placed on a special culture medium and incubated for 18 to 24 hours at 44. 5 degrees celsius, in aerobic conditions

    目前採用的薄膜過濾法是以濾膜隔濾海水中的細,然後將濾膜放到特殊的培養基上,在攝氏44 . 5度的有氧環境下培養18至24小時,其間濾膜表面隔濾的細獨特的藍綠色斑點大腸桿
  11. In the indices, cfu is colony formating unit, ntu is the unit for turbidity

    水質指標單位中, cfu為菌落形成單位數ntu為濁度表示單位。
  12. 2. among water quality index units, cfu is the unit for bacterial count, and ntu the unit for turbidity

    2 .水質指標單位中, cfu為菌落形成單位數ntu為濁度表示單位。
  13. Milk - enumeration of colony - forming units of psychrotrophic microorganisms - colony - count technique at 6, 5 ? c

    牛奶.嗜冷微生物菌落形成單位的計數. 6 . 5時的計數技術
  14. In the water quality indicators, cfu is the colony formation unit of bacteria. in the figures, nd means the concentration is too low to be detected by instrument

    水質指標單位中, cfu為菌落形成單位數數據資料中, nd表示濃度非常低,低到儀器無法檢測出來。
  15. Among the water quality indicators, cfu - 100ml means the number of colony forming units in 100 ml water ; ntu means nephelometric turbidity unit, and for drinking water, 1 mg l 1 ppm one part per million

    水質指標單位中, cfu 100ml表示每100毫升水樣中菌落形成單位數colony forming unit : ntu為濁度表示單位nephelometric turbidity unit 。對飲用水而言, 1mg l毫克每公升1ppm百萬分之一含量。
  16. Cork stoppers - enumeration of colony - forming units of yeasts, moulds and bacteria capable of growth in an alcoholic medium

    軟木塞.可在酒類介質中生長的酵母黴和細單位的計數
  17. It showed that bacterial colonies formed via number increase, exhausting nutrient, releasing waste, and results in environment far - from - equilibrium

    結果顯示細生長,消耗營養,排除廢物,導致環境遠離平衡。
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