菌體蛋白 的英文怎麼說
中文拼音 [jūntǐdànbái]
菌體蛋白
英文
mycoprotein-
They identified two bacteria, lactobacillus casei and lactobacillus plantarum, which can be added to beans so they cause minimal distress to those who eat them, and to those around the bean - lovers, marisela granito of simon bolivar university in caracas, venezuela and colleagues reported
研究人員發現,把兩種細菌酪蛋白乳酸桿菌lactobacillus casei和植物乳酸桿菌lactobacillus plantarum添加到豆子中,就可以讓其產生氣體的可能性變小。這樣豆子愛好者們和他們身邊的人就都能從中受益。The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times, and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant
用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體,在37條件下,洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體,包涵體在8mol l尿素中的溶解性非常好。A study on the enzymic hydrolysis of glu thalli protein in the production of monosodium glutamate
谷氨酸菌體蛋白的酶解試驗研究The highest antiviral litre of rpoifn - a was l l08iu / mi. the rpoifn - a protected pk - 15 cells against virulent hog cholera virus ( hcv ) infection in vitro
Sds - page分析表明,構建的重組表達質粒pqe30 poifn在大腸桿菌jm109中表達的rpoifn占菌體蛋白總量的20 26 。In order to produce monoclonal antibodies, first, several v. dahliae isolates were grown in liquid czapeak medium, after rinsing mycelia and eliminating zoospores, the fungal tissue was homogenized with the pestle in liquid nitrogen and then transferred to test tubes and was centrifuged in tris - hcl buffer
在制備抗原的過程中,首先液體振蕩培養了若干株棉花黃萎病菌,經過沖洗除孢子、液氮研磨,用tris - hcl抽提,再離心制得菌絲蛋白提取液,可作為電泳樣品。Two main parts were included in the present thesis. part i. cloning and expression cryigenes of bacillus thuringiensis in different host strains
一、蘇雲金芽胞桿菌殺蟲晶體蛋白基因在不同受體菌中的表達1Accumulation of single cell protein by purple non - sulfur photosynthetic bacteria in starch waste water treatment
光合細菌法降解澱粉廢水積累菌體蛋白的研究We can draw a conclusion that the mixture can promote psb reproduction and protein content
實驗結果表明,氣態混合物能夠促進光合細菌的生長,菌體蛋白含量明顯增加。And the expression product was an membrane - toxic immunotoxin. the activity determination in vitro indicated the product had significant killing effect to a43i cell of squamous epithelium carcin
破壁后提取ecoil中的目的產物會由於菌體蛋白的大量攙雜而使提純步驟繁瑣,降低得率。In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定,該基因為雞il - 2基因,其序列與sundick等報道的完全一致。在此基礎上,我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ ( tm ) ht中,構建重組表達質粒並進行確證性序列測定,重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ ( tm ) ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養, sds - page和westernblot分析顯示,表達的雞il - 2融合蛋白分子量約為18kda ,表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg. consisting 10 % of the total bacterial proteins, and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda, which consisting 35 % of total bacterial proteins. 5
Sds - page分析表明,重組質粒pet - 32a ( + ) - igf -在iptg誘導下表達分子量約30kda的融合蛋白,但其表達量不高,約為菌體總蛋白的10左右;重組質粒pet - 30a ( + ) - igf -在iptg誘導下表達分子量約14kda的重組蛋白,融合蛋白表達量約占菌體蛋白總量的35 。The reults of sds - page and western blot dermonstrated that the insoulable component of the induced e. coli culture contained protein 3a. the results of the study indicated that protein 3a existed in the form of inclusion body. the content of the expressed protein in the induced bacteria protein was 29. 2 % and 22. 1 % respectively
Sds - page和westernbolt結果證實,大腸桿菌菌體不可溶性蛋白中富含3a蛋白,且此融合蛋白的分子量符合預期設計,說明3a蛋白在表達產物中以包涵體的形式存在,所表達的蛋白含量分別占菌體蛋白的29 . 2和22 . 1 。After bacterium cells were treated by formaldehyde, surface proteins of gram - negative bacterium e. coli k12 were eluted little, but it had no influence on adsorption of snake venom proteins to bacterium cells. treatment by formaldehyde had little influence on snake venom absorption to and deabsorption from gram - positive bacterium streptococcus mutans, but absorbance of interaction protein with molecular weight of 47kda only adsorbed by streptococcus aureus was greatly decreased
結果表明,滅活后的革蘭氏陰性菌( e . colik12 )再與蛇毒相互作用時,洗脫下來的蛋白中菌體蛋白含量明顯減少,但不影響對作用蛋白的吸附;甲醛滅活對革蘭氏陽性菌( s . mutans和s . aureus )影響不大,只有s . aureus吸附蛋白中47kda的蛋白吸附量減少,不會影響作用蛋白中其它蛋白的吸附。Acid protease gave play to synergetic action in liquor fermentation manifested as dissolving the granules of fermentation materials, advancing microbial propagation, decomposing protein to produce flavoring materials, and degrading yeast tropina
摘要酒用酸性蛋白酶在白酒釀造的發酵過程中起協同作用,具有溶解發酵原料的顆粒、促進微生物繁殖、分解蛋白質生成香味物質、降解酵母菌體蛋白等多種功能。Price, a. c. et al. " inhibition of b - ketoacyl - acyl carrier protein synthases by thiolactomycin and cerulenin. " j. biol. chem. 276 ( 2001 ) : 6551 - 9
硫乳黴素與淺藍菌素對酮酰基酰基載體蛋白合成酶的抑制作用, 《生物化學期刊》 276 ( 2001 ) : 6551 - 9The p33, which is considered to be the precursor of p27 and coordinate to e. coli tgi expressed phl, is expressed and excreted at lag phase. then a splicing process is supposed occurred during the exponential phase resulting in production of mature phospholipase p27. the p32 p31 p30, p29 are believed to be intermediates of this splicing process
但在菌體生長進入穩定期后,僅產生p60和p27兩種成熟的磷酯酶,其中p33為p27的前體蛋白,與phl應為同種蛋白,經剪切作用產生一系列的中間產物p32 、 p31 、 p30 、 p29 ,最終產生成熟蛋白p27 。6 hours later, the amount of the expression yield of cp accounting for the total protein of the cells was 28. 9 % ( about 2mg / ml cp ). after sds - page, the recovery of cp added the same volume of incomp lete freund adjuvant emulsified completely, injected rabbits by subcutaneous injection. every rabbit was injected 2ml emulsified antigen ( 300ug cp ) once a week
重組表達載體導入宿主菌e . colibl21 ( de3 ) ,用終濃度為1mmol / liptg誘導, 37培養6小時后, cp基因的表達量達到最高,每毫升菌液含目的蛋白的量約為2mg ; uvi光密度掃描分析表明cp基因的表達量占細菌總蛋白的28 . 9 。In this pathway, irel, an er transmembrane protein kinase / endoribonuclease, is essential for viability during er stress. in contrast to yeast, the mammalian genome contains two homologues of ire1, irelctand ire1b, to sense the unfolded protein level
該基因的開放讀碼框( openreadingfiame , orf )為1383bp ,編碼蛋白的大小為50kd ,大腸桿菌ibeb基因編碼的50kd前體蛋白經分子內剪切加工形成大小為34kd的成熟表達產物,定位於細胞外膜。Advances in the rfsearch on crystalline inclusion proteins produced by symbiotic bacteria associated with entomopathogenic nematodes xenorhabdus and photorhabdus
昆蟲病原線蟲共生細菌胞內晶體蛋白的研究進展The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表達產物在細胞內外的精細定位研究表明,融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上,融合蛋白主要以不溶性包涵體的形式存在於細胞中,少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。分享友人