薄層電泳 的英文怎麼說
中文拼音 [bócéngdiànyǒng]
薄層電泳
英文
thin layer electrophoresis-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Varietal identification of oat avena spp. by ultrathin - layer isoelectric focusing of seed protein
超薄層等電聚焦電泳技術檢驗燕麥種子的真實性Thin layer electrophoresis apparatus
薄層電泳儀It owns more than 50 precision instruments, such as lc - ms, gc - ms, hpce, hplc, gc, ft - ir, uv, tlcs, aa, etc. there are about 15 thousand chinese and foreign books on relative specialities and more than 140 sorts of chinese and foreign journals in the library
擁有液-質及氣-質聯用儀原子吸收分光光度計毛細管電泳儀高效液相色譜儀氣相色譜儀紅外分光光度計薄層掃描儀全自動溶出儀等大型精密儀器150多臺件。By gel filtration on sepharose cl - 6b and cellulose acetate pellicle electrophoresis test, hpa and hpb were respectively a single strait symmetry apex and the result of electrophoresis assume a single spectrum strip
經sepharosecl - 6b柱層析和醋酸纖維素薄膜電泳檢驗， hpa和hpb均為單一狹窄對稱峰且電泳呈單一譜帶。分享友人