蘇力菌 的英文怎麼說
中文拼音 [sūlìjūn]
蘇力菌
英文
thuricide-
Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2
含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。But does not harm other organisms. the toxin gene in different strains of b. thuringiensis can affect different mixes of insects so seed makers can select the version that seems best suited to a particular crop
不同的蘇力菌菌株，各有不同的毒基因，影響的昆蟲也不同，所以種籽生產商可以針對特定的作物，選用最適合的抗蟲基因。The research results are summarized as following : 1. the relationship between gene types and the toxicity against s. exigua in order to construct satisfactory genetically engineered strain, it is first necessary to understand the relationship between gene types and the toxicity. nine strains containing crylc were chosen for study for this purpose
研究結果總結如下： 1菌株對甜菜夜蛾的毒力與基因類型的關系pcr擴增檢測了九個蘇雲金芽胞桿菌菌株所含殺蟲晶體蛋白（ insecticidalcrystalprotein ， icp ）基因，根據擴增結果將它們分為5種基因類型。4. effect of resolution vector on the expression of pesticidal crystal protein genes this work successfully introduced pesticidal crystal protein genes into crystal negative strain bmb171 by using resolution shuttle vectors. after resolution, the expression of cry1ac and cry 1c genes have no obvious change, but the expression of cry3a genes has great increase in the same condition
解離反應對殺蟲晶體蛋白基因表達的影響成功地利用解離載體將crylac10 ， crylc和cry3a基因導入蘇雲金芽胞桿菌無晶體突變株， crylac和crylc基因解離前後的表達量和殺蟲毒力未見明顯變化， cry3a基因在相同條件下則表達量有所提高，至於為何只對基因cry3a有作用尚不清楚，國內外也未見有人作相關報道。Microcalorinetric study on b. thuringiensis by using an lkb - 2277 bioacitivity monitor, the thermogenic curves of different b thuringiensis strains ybt - 833 and ybt - 833 - 2 - i, have been determined. the metabolism heat output revealed the heat output was correlated to the yield of the protein, the higher yield protein, the less heat output. a microcalorimetric technique based on the bacterial heat - output was explored to evaluate the effect of various promoters and different plasmid original replicons on the expression of gfp
不同蘇雲金芽胞桿菌基因工程菌的微量熱變化利用生物活性檢測器lkb - 2277研究殺蟲晶體蛋白含量不同的兩株菌ybt - 833 、 ybt - 833 - 2 - 1的熱動力學變化，發現菌體合成殺蟲晶體蛋白的過程是一個耗能的過程，殺蟲晶體蛋白產量高的菌株向外釋放的代謝熱少，反之亦然。1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers
將cry基因的高保守區的cry a （ a ） ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ，獲得了能在體外轉錄的rna探針載體pbpl - 1 ，用該載體制備的rna探針具有特異強，背景清楚，省時省力等優點，已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。分享友人