蛇毒蛋白 的英文怎麼說
中文拼音 [shédúdànbái]
蛇毒蛋白
英文
echidnotoxin- 蛇 : 蛇名詞(爬行動物) snake; serpent; mole catcher
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 蛇毒 : snake venom; venin; venene蛇毒蛋白 echidnotoxin; 蛇毒抗毒血清 antivenin; antivene; 蛇毒抗蛇毒合劑...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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Snake venom, cytotoxin and plant poisonous protein such as ricin all have the defence function
蛇毒、細胞毒素及植物毒蛋白質,如蓖麻蛋白等,均具有防禦功能。Purdue uniersity researchers bound a complex nanomolecule, called a dendrimer, with a glowing identification tag that was deliered to specific proteins in liing enom cells from a rattlesnake
普渡大學研究人員成功合成了一種稱作樹狀高分子的復合納米分子,隨后他們以此對響尾蛇活性毒液細胞中的一種特殊蛋白進行了鑒定。In aqueous solution, interaction between crude venom and different bacteria including gram - positive bacteria, i. e. streptococcus mutans, streptococcus aureus, and gram - negative bacteria, i. e. e. colik12, was investigated in detail. molecule weights of multiple proteins were identified as 110kda, 47kda, 24kda, 23kda and 15kda by sds - page, respectively. it had little influence on extraction of snake venom proteins by the same cells of streptococcus mutans even used for 10 cycles
其次,在利用s . mutans分離細菌特異性抗體的基礎上,首次利用細菌篩選作用蛋白,將革蘭氏陽性菌( s . mutans和s . aureus )和革蘭氏陰性菌( e . colik12 )分別與蘄蛇蛇毒相互作用,發現有五種蛇毒蛋白能夠與細菌相互作用,其分子量分別為110kda 、 47kda 、 24kda 、 23kda和15kda ;同時表明s . mutans菌體能夠多次與蛇毒相互作用,並且對作用蛋白的結合沒有影響。Ahylysantinfarctase from snake venom extract with lower fibrinogen and blood viscosity of the material
蝮蛇抗栓酶從蝮蛇蛇毒提取的具有降低纖維蛋白原和血液粘滯度的物質。Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 ( a ) +中,在t7啟動子下表達出融合蛋白,融合部分為硫氧還蛋白,位於類凝血酶基因上游,並在其n端帶6xhistag標簽以利於表達產物的分離純化,經熱激轉化至宿主菌bl21 ( de3 )中, iptg誘導斗小時后收獲菌體。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性,設計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當的限制性內切酶位點插入表達載體pbv220 ,分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆;進一步通過pcr技術構建echistatin的融合表達基因克隆。After bacterium cells were treated by formaldehyde, surface proteins of gram - negative bacterium e. coli k12 were eluted little, but it had no influence on adsorption of snake venom proteins to bacterium cells. treatment by formaldehyde had little influence on snake venom absorption to and deabsorption from gram - positive bacterium streptococcus mutans, but absorbance of interaction protein with molecular weight of 47kda only adsorbed by streptococcus aureus was greatly decreased
結果表明,滅活后的革蘭氏陰性菌( e . colik12 )再與蛇毒相互作用時,洗脫下來的蛋白中菌體蛋白含量明顯減少,但不影響對作用蛋白的吸附;甲醛滅活對革蘭氏陽性菌( s . mutans和s . aureus )影響不大,只有s . aureus吸附蛋白中47kda的蛋白吸附量減少,不會影響作用蛋白中其它蛋白的吸附。To fibrin therapeutic use extracted from the venom of the anticoagulant effect of a substance, can be reduced fibrinogen and blood viscosity, for the treatment of arteriovenous thrombosis was good results
去纖維蛋白治療應用從蛇毒中提取的一種抗凝作用的物質,可以降低纖維蛋白原和血液粘度,用以治療動靜脈血栓獲得良好效果。分享友人