蛋白分選 的英文怎麼說

中文拼音 [dànbáifēnxuǎn]
蛋白分選 英文
protein sorting
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants

    血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。
  3. After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including estss analogous to 11. 5kd antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library

    在仔細分析了它們在免疫系統中的重要性和在對蝦中出現的可能性之後，從中選出了17條可能編碼抗菌肽，溶菌酶，凝集素、絲氨酸蛋白酶及其抑制劑，抗凍蛋白等蛋白質的序列，以此為依據設計引物，在中國對蝦的血液和頭胸部cdna文庫中擴增相應的序列。
  4. The development of a multicellular organism results from the selective expression of genes and the maturation of proteins is dependent on molecular chaperons

    多細胞生物體發育的實質是基因的選擇表達性，表達蛋白的成熟則有賴于分子伴侶的協助。
  5. Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4

    Sds page分析得到3條主要蛋白帶，剪下這三條帶進行膠內賴氨酸內切酶的消化，通過高效液相色譜分離肽段，選擇性進行肽段的氨基酸序列測定。
  6. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  7. Hi this thesis, the setting time of various retarders had been tested, in which three retarders with good retarding action, citric acid, sodium tripolyphosphate, bone glue are respectively chosen from three types of retarders, hydroxyl carboxylic acid type, phosphate type and protein type, and the strength and setting time of gypsum added with them are determined. effect of some factors, such as ph value, fineness of hemihydrate, type of gypsum on the retarding action of the retarders is also studied

    本文對多種緩凝劑進行凝結時間測試，選擇效果良好的羥基羧酸、無機鹽和蛋白質類型的三種緩凝劑：檸檬酸、多聚磷酸鈉和骨膠，測定它們對石膏凝結時間、強度等宏觀性能的影響，並分別研究ph值、石膏細度和石膏種類等因素對它們的緩凝效果的影響。
  8. Except the incomplete maturation of spermatid nuclear and oocyte activation, idendification of a live spermatid is a pivotal procedure. it is difficult to distinguish round spermatids from other round cells such as spermatocytes, monocytes, polymorphonuclear leukocytes and so on

    除精子細胞的核蛋白不完全成熟及卵子激活不足等因素外，如何正確選擇存活精子細胞是個難題，如圓形精子細胞與精母細胞、單核細胞和多形核白細胞等其他圓形細胞的區分就比較困難。
  9. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  10. The biological characteristics of mycelia from phellinus igniarius and culture media were studied. two kinds of culture media were suitable for the growth of mycelia. the result indicated that the culture medium with potato as nitrogen source and saccharose as carbon source was suitable for collecting mycelia, and the culture medium with peptone as nitrogen source and solvable amylum as carbon source was suitable for conservation

    為了最大限度地保存菌種的活力，以提高菌絲體的質量及菌絲體內活性成分的累積，本文通過對比研究，進一步對其生長基質進行篩選，明確了兩種適于桑黃菌絲生長的固體培養基：以馬鈴薯為氮源、蔗糖為碳源的培養基較適用於菌絲收集，以蛋白腖為氮源、可溶性澱粉為碳源的培養基較適用於菌種的保藏。
  11. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。
  12. The two cdna fragments, ap - 2a full length cdna and ap - 2 a cdna fragments were inserted in frame into gst gene fusion system. with an ap - 2a monoclonal antibody we detected the expression of gst - ap - 2a

    將這兩段篩選所得cdna片段和ap - 2 cdna全長及ap - 2 cdna的分段形式用gst基因融合系統進行表達，並用ap - 2的單克隆抗體檢測了gst - ap - 2融合蛋白表達的情況。
  13. The topics include : structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease

    主題包括：基因、染色體與基因組的結構和功能；來自於基因重組、突變和篩選的生物變異；族群遺傳學；運用遺傳學的方法分析蛋白質的功能，基因的調控和遺傳性疾病。
  14. Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but

    通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1
  15. This text used different biological enzymes to work on dry milling maize powder, by testing the viscosity shown on rva, selected biological enzymes amounts and variety based on viscosity curve, and analyzed their mechanism simply

    摘要採用蛋白酶、脫支酶、乳酸菌胞外酶處理玉米麵粉，用rva測其糊化曲線，篩選出對糊化性質有所改善的生物酶及其用量，並分析其改良機理。
  16. The article addresses special properties of various silks and / or silk composite fabrics, dyes, and dyeing methods for dying of silk / cotton, silk / linen, silk / wool, silk / polyester, silk / spandex, silk / soybean protein fiber and silk / bamboo fiber, as well as precautions with the aim of covering the recent advances in the field in recent years and providing practical guidance for the production

    摘要針對蠶絲復合織物各組分纖維的特點，綜述了近年來有關蠶絲棉、蠶絲麻、蠶絲羊毛、蠶絲滌綸、蠶絲氨綸、蠶絲大豆蛋白復合纖維、蠶絲竹纖維等蠶絲復合織物使用的染料種類、染色方法的選擇及在生產中的注意事項，以期全面反映該領域的研究進展，對實際生產有一定的指導意義。
  17. But, because children loves to hit more, amuse, move more, jiashangwei gives birth to intellectual control very little, the needle that manages ear department easily ( medical ), cause contamination infection, accordingly, in apply after treating, must coach child note, ask parents is timely circumstance of examination ear needle, notice ear ministry is wholesome, want to notice to change dietary structure at the same time, low carbohydrate of choice high protein, low adipose food, encourage its to increase an activity, ability achieves anticipated curative effect, of parents it is very important to cooperate closely

    但是，由於兒童多喜愛打逗，多動，加上衛生知識把握甚少，易於弄掉耳部的針（藥） ，並引起污物感染，因此，在施治后必須指導孩子注重事項，並要求父母適時檢查耳針情況，注重耳部衛生，同時要注重改變飲食結構，選擇高蛋白低碳水化合物、低脂肪的飲食，鼓勵其增加活動，才能達到預期的療效，家長們的密切配合十分重要。
  18. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  19. Mammalian cells are among the best systems for biopharmaceutical production due to their excellence in post - translational modifications. the products they produced are much more similar to their natural forms than those produced by prokaryotic, yeast or insect cells

    哺乳動物細胞表達系統具有準確的轉錄后修飾功能，表達的糖基化蛋白藥物在分子結構、理化性質和生物學功能方面最接近天然蛋白分子，是目前重組糖蛋白藥物生產的首選體系。
  20. Sectionii : to research and establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies. to raise separate efficiency and biological active efficiency is especially important in the purification model of recombinant proteins. in accordance with these questions, we raise inclusion bodies purity before the purification as far as possible, and select the suitable chromatogram technology

    研究並建立一種包涵體中高效分離純化目的蛋白的優化模式重組蛋白分離純化工藝中分離效率的提高、生物活性效率的提高及工藝的穩定性是尤其重要的，針對這些問題，我們在層析純化前盡山西醫科大學生物化學與分子生物學2003屆碩士學位論文可能地提高包涵體純度，並選擇合適層析方法的同時合理的組合色譜分離單元。
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