蛋白同化作用 的英文怎麼說
中文拼音 [dànbáitónghuàzuòyòng]
蛋白同化作用
英文
protein assimilation-
Inhibition by zn2 + and mn2 + additive, and almost 100 % of t he activity was inhibited under their physiologically significant concentrations. these results suggest that the protease is possibly regulated by divalent cations in vivo
蛋白酶的活性受到某些二價金屬陽離于的抑制，比如zn訃、 mg卜、 hg熒光發射光譜結果顯示，在不同的抑制劑作用下，蛋白酶的高級構象可能發生了較大變化。Compared with the former study of interaction between varieties of vertebrates hemoglobin and human hemoglobin, human fetal hemoglobin ( hb f ), the results support the conclusion about the boundary of interaction between vertebrates hemoglobin and kinds of protein molecule in the electric field is the reptilia animals and enrich the theory of hemoglobin molecular evolution
同前面所進行的各種脊椎動物血紅蛋白分別與人血紅蛋白、胎兒血紅蛋白相互作用的研究對照比較，進一步驗證脊椎動物血紅蛋白與各種蛋白分子在電場中的相互作用的分水嶺可能位於爬行綱動物，豐富了血紅蛋白分子進化理論。According to the physical - chemical property of the wool and the actions of the chemilacs on the wool in the solution, we take all kinds of measures such as bleaching, osmosis, setting, etc, and combine with the experiments data of the wool under the different chmical conditions. therefore, we have found the best way of the wools chemical mercerization
根據羊毛蛋白質纖維的理化性質及其在水溶液中與化學藥劑發生作用的情況，我們採取了各種漂白、滲透、定形等措施，並結合羊毛在不同化學條件下絲光情況的實驗數據，得出最佳化學絲光羊毛的工藝路線。In this paper, the roles of iron in different pathways of protein nitration are introduced, and the results indicated that microelement iron in vivo plays an important role in protein nitration
本文介紹了鐵在不同酪氨酸硝化途徑中的作用，結果提示體內的微量鐵對蛋白質硝化起著重要作用。Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點，包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等，拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能，而磷酸化位點是arc1參與信號傳導過程所必需的。Rab7 mediates transport of proteins from early endosome to late endosome or fusion of early endosome with late endosome, while rab9 mediates the retransport of m6pr from late endosome to trans - golgi network. the gtp / gdp - binding ability and intrinsic gtpase activity of rab proteins make them exist in rab - gtp or rab - gdp status. the switching of rabs between gtp - bound ( active ) and gdp - bound ( inactive ) forms requires other regulatory proteins, including guanosine exchange factor ( gef ), gdp dissociation inhibitor ( gdi ) and gtpase activating protein ( gap ), and lays the foundations of rab function
Rab蛋白家族成員和其他小g蛋白一樣可以結合gtp gdp ，在自身gtp酶的水解作用和其他蛋白（ gef 、 gap 、 gdi ）的調節作用下發生gtp （活化狀態）和gdp （無活性）結合形式的轉變；同時rab蛋白也在膜結合性（ membrane - bound ）和胞漿可溶性（ cytosolic ）之間變化，一個變化過程對應著一個功能循環，接著rab蛋白回到初始狀態參與下一個循環。So rbp is regarded as sensitive biomarker for the clinical diagnose of these diseases. it can also reveal the early change of nutrition state of protein
因此，臨床上將其作為診斷某些疾病的輔助指標，同時也用來評價蛋白質營養狀況的早期變化。Normal method was used to purify the polysaccharide of spirulina platensis, pspi ( mw23000 ) was got. pspi had cooperativity for immobilized pc to inhibit pancreatic cancer cells
在第四章中，研究了光固定化藻藍蛋白在藻多糖的協同作用下對人胰腺癌細胞sw1990生長的抑制作用。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。Anti - if - protein antibodies were used as probes for immuno - fluorescence, and the reactions of them with different parts of the cell were detected, which suggested the possibility of the existence of the intermediate - like filaments in the cytoplasm. those proteins homologous to the antibodies distributed regularly in the protoplasm. to characterize the corresponding proteins, sds - page and immunoblots were utilized. the 21, 23, 33 and 68kd proteins were distinguished among the diverse protein constituents of the cell. some of these proteins also showed the cross - reactivities with anti - if - proteins antibodies derived from higher organisms. these two evidences both contributed to the homology of some proteins in
以抗中間纖維蛋白抗體作為探針進行免疫熒光實驗，得到細胞內不同部位的陽性反應，暗示原生質中可能存在類中間纖維。這些同源蛋白的胞內分佈具有一定的規律性。進一步採用sds - page和免疫印跡技術研究它們的生化性質，發現4種主要蛋白明顯有別于胞內其他蛋白組分。Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on
該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。Cellular interaction takes place, changing the amounts of various chemical reactions, proteins, and so on in the cellular structure and actually altering the metabolism of the body
在細胞構造里發生細胞的交感作用，正改變各種不同的化學反應、蛋白質等等而實際正改變著身體的新陳代謝。The protein, fat, carbohydrate, vitamin, inorganic salt, water and meal fiber in the food can not be fully utilized unless people eat diversified food with proper proportions for joint effects
而食物中的蛋白質、脂肪、碳水化合物、維生素、無機鹽、水和膳食纖維只有「種類齊全、比例適當、協同作用」 ，才能充分發揮食物的營養作用。This paper stuffed with twelve important grain and vegetable crops, studied the injury symptom, dose reaction, injury threshold value and influential factor of main pollutant so2 on various plants, tested the dynamic transformation of pod, cat, mda, soluble protein, free pro and chlorophyll of resistant plant and sensitive of these physiological biochemical transformation with plant resistant ability. meanwhile, simply studied the protective role of the five compounds on plant. the result indicated the followings
本實驗以12種重要的糧食和蔬菜作物為研究對象，研究了主要大氣污染物二氧化硫（ so _ 2 ）對不同植物的傷害癥狀、劑量反應、傷害閾值以及影響因素，測定了抗性和敏感植物在受到so _ 2污染后植物體內過氧化物酶（ pod ） 、過氧化氫酶（ cat ） 、丙二醛（ mda ） 、可溶性蛋白質、游離脯氨酸和葉綠素的動態變化，並分析了這些生理生化變化和植物抗性的相互關系，同時還對5種化合物溶液對植物的保護作用進行了初步研究，結果表明： 1Tetraploid black locust is a variety has been bred in south korea, the leaves of which are 3 times larger than those of diploid varieties and contain 1. 4 times more protein. and it has no prickles on its surface. it can be used not only as pioneer species in environmental beautifying and ecological restoration, also as fodder, bee forage, timber production, and so on
四倍體刺槐是韓國培育出的刺槐新品種，屬無刺類型，其葉片大小為普通二倍體刺槐的4倍，蛋白質含量為1 . 4倍多，不但可以作為先鋒樹種廣泛應用於環境美化和生態建設中，還可作為飼料、蜜源和木材等不僅具有較好的生態效益，同時還具有很高的經濟價值。In this work, effects of sfamnpv on mitochondrial and er in sl - 1 cells were studied. the main results were as follows : effect of sfamnpv ( moi = 6 ) on the function of mitochondrial in sl - 1 cells was investigated by mtt assay and membrane potential was assayed by both flow cytometry and confocal laser scanning microscope
完成的工作包括以下幾個方面： （ 1 ）細胞內線粒體的變化通過mtt法研究病毒感染對細胞線粒體功能的影響，同時用流式細胞儀和共聚焦激光顯微鏡研究線粒體膜電位（ m ）的變化， western檢測2種凋亡調控蛋白cytoc和bcl - 2蛋白的變化。Efficiency : richly contain olive oil essence, wheat germ oil, mastic, tea polyphenol, rose essential oil and collagen, activate basal cells, improve the formulation of collagen and elastin in cuticle, promote the support of cuticle to epidermis, completely improve skin quality, promote metabolism, smoothen, moisturize and nourish skin, delay ageing, firm droopy skin, make skin tender, white, moist and firm
功效：蘊含豐富的橄欖油精華、小麥胚芽油、乳香、茶多酚、玫瑰精油和膠原蛋白，能活化基底層細胞及提高真皮層膠原蛋白和彈力蛋白的合成，加強真皮層對表皮層的支撐作用，徹底改善膚質，同時促進肌膚代謝功能，平滑肌膚，保濕滋養、延緩衰老，收緊松馳，令肌膚回復嫩白，潤滑細膩有彈性。分享友人