蛋白法 的英文怎麼說
中文拼音 [dànbáifǎ]
蛋白法
英文
western blot-
First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。Preliminary studies on a new method abstracting pupal albumen
一種提取蛹蛋白的新方法初探The albumen - detected technology is based on the uv - absorption principle
依據紫外吸光度分析法,對蛋白質樣品進行電泳分離與紫外檢測。Interaction of bovine serum albumin with benzoate
光譜法研究苯甲酸鈉與牛血清白蛋白的作用The point of this brief historic reiew, if there is one, is that the meaning of a word can change, and that the meaning of albuminuria has changed as the methods to detect and characterize proteins hae eoled
如果說這篇簡短的有歷史意義的綜述有要點的話,那就是它指出了一個單詞的含義是可以變化的,而且隨著檢測和鑒定蛋白質方法的變化,白蛋白尿的含意也發生了改變。We prospectively investigated whether measurements of carotid intima - media thickness in the presence or absence of albuminuria in patients with stable chest pain syndromes can be used as a noninvasive test algorithm for prediction of significant coronary artery disease
前瞻性研究穩定性胸痛綜合征患者在有/無蛋白尿時的頸動脈內膜中層厚度測量能否作為預測重要冠狀動脈疾病的一種無創性檢查方法。A novel protein immobilization approach for piezoelectric immunosensors based on the sam of derivative aa has been proposed, which is formed by coupling merca ptoprorionic acid ( mpa ) to alginic acid sodium salt via edc and nhs. ( 1 ) the tr antibody is immobilized via edc and nhs. comparing with the mpa sam method, this new approach shows greater frequency response and high sensitivity
應用edc和nhs使褐藻酸鈉與胱胺偶合形成衍生褐藻酸鈉作新的自組裝材料,用於蛋白固定,應用這一新方法, ( 1 )實現了轉鐵蛋白抗體分子的固定,並對轉鐵蛋白參考血清進行了測定,該方法可在0 . 08 25 . 7 g / ml范圍內對轉鐵蛋白進行測定。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Fat, protein, aminophenol, trace elements in amygdalus ledebouriana schleche were determinated by chp ( 2005 edition ) and national standard methods, natural benzaldehyde and amygdalin were determinated by uv absorb method and hplc method respectively
分別用索氏抽提法、微量凱氏定氮法測定野巴旦杏中脂肪、蛋白質,用氨基酸分析儀測定多種氨基酸含量,原子吸收法測定多種元素,紫外法及反相高效液相法測定天然苯甲醛及苦杏仁苷。These results suggested that high litre antiserum had been acquired. 5. immunoassay of plant ferritin
這表明通過上述方法制備的豌豆鐵蛋白抗血清具有較高的效價。Yellow powder insect stem article certain fat f aridity, smash insect powder, can prolong conservancy period and raise albumen content and quality
黃粉蟲干品經技術方法提取一定脂肪后的乾燥、粉碎蟲粉,可以延長保存期並提高蛋白含量與質量。Absorption, fluorescence emission and excitation spectra of five pigment - protein complexes were determined. photosynthetic electron transfer was measured from the dcip photoreduction. p700 concentration was assayed from the ferricyanide - oxdised minus ascorbate - reduced difference spectrum
測定裙帶菜各色素蛋白復合物的吸收光譜、熒光發射光譜和熒光激發光譜,並進行了dcip的光還原測定和化學法的氧化還原差示光譜測定。Standard test method for isoaspartic acid in proteins : method for the determination of asparagine deamidation products
蛋白質中異天門冬氨酸的標準試驗方法:天門冬酰胺脫酰胺基製品的測定方法By using the similar separation and purification methods, a nifh and a nife mofe protein were obtained from azotobacter vinelandii mutants dj54 and dj35. the results of the complement activity experiments showed that a nifh mofe could be activated by femoco
從棕色固氮菌突變種dj _ ( 54 )中得到了nifhmofe蛋白;並參與了棕色固氮菌突變種dj _ ( 35 )的nifemofe蛋白的分離純化,所用方法與nifb ~ - mofe蛋白的分離純化相似。The development of these new varieties ( strains ) indicated that using the summer type variety from low latitude area as the high - protein parent, and local high - yield accessions as the recurrent parental lines in modified backcross is an efficient strategy for high - protein, high - yield soybean breeding
育種實踐證明,選擇低緯度地區的夏大豆品種(系)作高蛋白基因源,創造高蛋白中間材料,採用本地豐產材料進行修飾回交,是高蛋白、高產大豆育種的一種有效方法。In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中,利用pcr克隆的方法擴增出bar基因全長,並在原核表達系統中表達,鑒定表達蛋白的活性,將能夠正確編碼ppt乙酰轉移酶的bar基因片段,經過適當的修飾構建入真核表達載體。We find that bt cotton has different bt toxin protein content in different tissue and growing period using elisa method. leaves and flower petals contain more bt toxin protein than boll and bracteal leaves
用elisa方法檢測bt棉殺蟲蛋白的含量,結果表明,棉葉和花瓣殺蟲蛋白含量最高,鈴和蕾次之。Methods : to observe with cyanomethemoglobin and indirectly measurement
方法:以氰化高鐵血紅蛋白法及直接測定法觀察。The results show that protein and multi vitamin and inorganic salt are insufficient ; the rate of anemia is 67. 81 %, and only 6. 70 % of hair iron content is low to normal level
採用五日回顧法進行了膳食調查,氰化高鐵血紅蛋白法測定血紅蛋白值及示波極譜法測定了發鐵含量。According to the result showed at 280nm and at 490nm, in the comparison of whether protein absorption top and sugar quantity top overlapped, glycoproteins would be detected preparatorily, and as a result, tubes in two distinct areas had glycoproteins by this method. proteins were precipitated with trichloroaceticacid and with cold acetone, and glycoprotein was determined from sds - gel
再從各收集管的收集液中,用三氯乙酸沉澱蛋白法、冷丙酮沉澱蛋白法相結合濃縮、制備蛋白樣品,進行sds ? page ,對sds膠進行pas糖鏈染色鑒定糖蛋白,並從茶樹葉分級蛋白中準確地鑒定出兩個區域的收集管中含有多種糖蛋白。分享友人