蛋白波 的英文怎麼說
中文拼音 [dànbáibō]
蛋白波
英文
protein wave-
Malonaldehyde, a marjor product of the peroxidation of polyunsaturated lipids, may play an essential role in the formation of the fluorescent products in lipofuscin - like fluorescent pigments and in the blood viscosity increase. fluorescence and non - enzymatic browning were observed in reactions between ascorbic acid ( asa ) and amino acids ( aa ) as well as in reactions involving asa autoxidation and / or polymerization in the presence of trace amounts of adventitious iron
在同時進行的熒光測定中,我們發現,丙二醛( 20mm )能迅速地降低蛋白溶液的典型熒光( 280nm 350nm ) ,而就此產生在395nm的波長激發下,發射出波長為460nm的熒光,造成蛋白質的變構變性。6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析, blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟,得到聚丙烯酰胺凝膠電泳為單一蛋白區帶,比活為1 . 46u mg的酶制劑。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型,主要採用抗fos 、膠質原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。Study on ultrasonic extraction of wheat germ protein
超聲波法提取小麥胚芽蛋白的研究It implies that gene expression in plant cells might have changed under the stimulation of sound wave. thirdly, the cell cycle of protoplast of control group and stimulated group ( stimulating for 9 days ) was estimated by flow cytometer. the results showed that the number of cells of stimulated group decreased in g0 / g1 phase and increased in s phase
圍繞中心法則分別測定了dna 、 rna和可溶蛋白質的含量,發現聲波刺激組的dna含量變化不明顯,而rna和可溶蛋白質的含量都有所升高,且以刺激9天的實驗組最為突出,表明在強聲波的作用下,有關應力響應的轉錄因子被啟動,轉錄水平提高,從而翻譯合成較多的蛋白質,促進植物的生長發育。The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test
經超聲波裂解,用尿素溶解包涵體,電泳純化后,利用elisa檢測vp _ 1外殼蛋白,表明具有一定抗原性;第四部分:應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交,來檢測那v的rna 。Secondly, analysis of peroxidase isoenzyme with polyacrylamidedel electrophoresis for was performed in order to investigate the changes of gene expression under sound stimulation. it could be seen from electrophoresis gel that each group had 6 enzyme bands. new enzyme band in pod electrophoretogram was n ' t detected for stressed groups
此外,在部分實驗組的培養基中加入不同濃度的蛋白質合成抑制劑環己亞胺酮( chm )后發現, pod和cat的活性有所降低,暗示著聲波處理使保護酶活性升高的原因可能是聲波處理促進了細胞內酶的合成。The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned
從陽性克隆子中提取克隆片段,經序列測定分析,結果表明,克隆片段的cdna大小為816bp ,編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank , embl , ddbj , pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索,結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ;蛋白質氨基酸序列的同源率為90 95 ,表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel
融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease ( ppase )酶切,酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后,得到純度較高的的m - centrin 。Identification of vimentin in subline coc1 ddp by maldi - tof mass peptide map analysis
亞細胞中波形纖維蛋白的肽質譜指紋分析The results show that protein and multi vitamin and inorganic salt are insufficient ; the rate of anemia is 67. 81 %, and only 6. 70 % of hair iron content is low to normal level
採用五日回顧法進行了膳食調查,氰化高鐵血紅蛋白法測定血紅蛋白值及示波極譜法測定了發鐵含量。In the case of uncorrelated noises, it is shown that only the fluctuation of degradation reaction rate can induce a switch process, and the mean first passage time ( mfpt ) between the high concentration state and low concentration one is decreased when the noise intensity of degradation reaction rate is increased
可以看到源於蛋白質基本合成率的噪聲強度不能引起基因狀態的轉變(即基因的開關)而源於降解率噪聲強度則能夠實現這種開關。當進一步研究在降解率波動作用下基因從一個態躍遷到另一個態的平均首通時間( mfpt )時可以看到此時隨著這個波動強度的增加, mfpt是單調遞減的。Green fluorescent protein ( gfp ) was extracted from jellyfish ( aequerea victoria ) of ocean invertebrate. gfp can emit green fluorescent when it is illuminated by light of suitable wavelength. the emissions of fluorescence need not any additional factors, such as substrate, supplementary factor
綠色熒光蛋白( greenfluorescentprotein , gfp )是來源於多管水母( aequoreavictoria )等海洋無脊椎動物的一種蛋白質,該蛋白質在體外經適當波長的光激發便發出綠色熒光,並且這種熒光的發射不需要任何底物和輔助因子的誘導。The polarographic catalytic waves of organic compounds, such as protein, flavone, steroide, quinine, - unsaturated carbonyl compound, nitrogen - containing substances and charged surfactants, and their application are briefly introduced
簡要介紹蛋白質、黃酮類化合物、甾族類化合物、醌類化合物、 ,不飽和羰基化合物、含氮化合物和荷電表面活性劑等不同種類有機化合物的極譜催化波及其應用。Adding the acetyl - coa to the protein mixture of phaa, phab and phac, the mixture was analysed with the wavelength of 230 - 240nm. the results showed that the absorption peak for pha could be found at the wavelength of 235nm. from all facts given above, it could be confirmed that three cloned genes have bioactivity and biological function synthesizing pha
將phaa 、 phab和phac基因表達的蛋白產物混和后,加入底物乙酰coa ,於230nm ? 240nm波長下,對反應產物進行掃描,發現在波長235nm處有明顯的pha特異吸收峰;表明混和物中有pha的產生,所表達的蛋白具有生物學活性和特定功能,證實所克隆的phaa 、 phab和phac就是合成pha所需的三個基因。Include : liver function test, afp and ultrasound upper abdomen include liver, gallbladder and spleen
(包括肝功能甲胎蛋白( afp )及上腹部超聲波The temporal and spatial expression of gfap and vimentin of olfactory system of xenopus laevis during metamorphosis
變形期非洲爪蛙嗅覺系統膠質原纖維酸性蛋白和波形蛋白的時空表達In this paper, how to prepare cp i and identify physico - chemical property had been studied, and uva oxidative damage l929 was perliminary discussed. these datas provided theoretic foundation and scientific proof for further research and development of this product. pigskin was used as material
本文圍繞型膠原蛋白( cp )的制備及其理化性質進行研究,同時在其防止長波紫外線( uva )對細胞的氧化損傷方面進行了初步探索,為cp的進一步研發提供理論基礎和科學依據。With bacterial cgc as main subject, the tests had been done to elucidate mechanism of self - organization for macroscopic rhythmic structure. the dynamics of cgc forming was observed by special techniques of waving culture and microscopic culture ; the differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning ; integrity of cell wall was examined under tem ; outer membrane protein was analysed by sds - page and various substance and factors for cgc formation were determined
採用特殊的波動培養和顯微培養技術觀察潛生體形成動態;應用原子力顯微鏡掃描,比較細菌潛生體與繁殖體在細胞壁外層結構和鞭毛數量的差別;用透射電鏡觀察細胞壁完整性,以十二烷基硫酸鈉?聚丙烯酰胺凝膠電泳分析外膜蛋白的改變,並通過實驗分析多種物質和因素對潛生體形成的影響。This very strong absorption of proteins at these wavelengths has been used in protein determination
在這些波長的蛋白質的強烈吸收被用於蛋白測定。分享友人