蛋白細胞分離 的英文怎麼說

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蛋白細胞分離 英文
albumincytological dissociation
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. We focused on the following aspects ; 1 ) we first assayed the expression of complement receptors and complement - associated molecules on distinct subsets of dendritic cells during their development in order to understand the physical basis of the sensitivity of dendritic cells to complement and its split products ; we next studied the effects of complement activation on the survival of dendritic cells during their development ; and finally examined the effects of the whole complement system, focusing on the ability of one of the split products of complement activation, c5a, and its first subcomponent - c1q, to influence chemotaxis of dendritic cells, as well as allo - t cell stimulatory activity of dc

    我們通過免疫磁珠分離了兩種人dc前體，即髓系來源的單核細胞（ monocytes ， mo ）和淋巴系來源的漿細胞樣dc （ plasmaeytoiddendriticeells ， pdc ） ，對這兩個不同dc亞群進行體外誘導培養，使其處于不同的分化發育階段，然後檢測了其表達補體受體一cd35 （ cri ） 、 cd21 （ crz ） 、 cdilb （ cr3 ） 、 cdlle （ cr4 ） ，補體調控蛋白一cd46 、 cd55 、以及部分補體片斷分子受體一c3ar 、 csar 、 clqrp的水平。
  2. When the cells synchronized at a late cytokinesis stage were treated with w7, the dissolving of the midbody was delayed ; furthermore, we found that the dissociation of y - tubulin from the midbody was also inhibited

    W7處理同步化在胞質分裂後期的細胞，不但使中體解聚發生延遲，而且抑制了y一微管蛋白與中體的解離。
  3. The mensuration of the biologic activity of the formica rufa linnaeus polypeptides. in the part, we used the experiments in vitro studies, the lymphocyte transformation, to mensurate the biologic activities of the polypeptides whose contents are higher than others ". the conclusion is the polypeptides have the immunological activity

    紅褐林蟻多肽蛋白質的生物活性測定本部分用體外實驗的方法? ?淋巴細胞轉化實驗對分離、純化所得含量較高的多肽蛋白質的生物活性進行測定，結論為所得到的多肽具有抑制淋巴細胞轉化的免疫活性。
  4. Expanded bed adsorption ( eba ) is a novel bioseparation technique, which integrates clarification, concentration and initial purification into a single unit operation. it enables proteins to be recovered directly from unclarified cultivations of microorganisms or cells and homogenates of disrupted cells, without the need for prior removal of suspended solids. matrix is the principal " hardware " pillar supporting the successful application of eba

    擴張床吸附（ eba ）技術是一種新型的生化分離技術，它集成了固液分離、濃縮和初期純化於一步單元操作之中，可以直接從含有細胞和細胞碎片的發酵液或培養液中提取目標蛋白，而不必事先除去懸浮的固體顆粒。
  5. It is interesting that pma plus calcium ionophore a23187 can inhibit pma - induced pta1 expression, and this effect ca n ' t be reversed by calcmeurin inhibiter fk506. pta1 mabs can inhibit ctl activation and differentiation in mixed lymphocyte culture system when added at the beginning of the culture but can induce platelet activation and aggregation in the fc dependent manner. in 1997, pta1 cdna was cloned from cdna library of tpa activated jurkat cells, which belongs to immunoglobulin superfamily ( igsf ) with two v - like domains of extracelluar region of pta1

    Il - 2 、 tnf - 、 pma可以使t細胞pta1表達上調， tgf -可以下調pta1的表達，而pma加上鈣離子載體a23187可以顯著抑制pma的上調作用，且這種抑制作用不被calcineurin抑制劑fk506所逆轉， 1997年burns教授從pma活化的jurkat細胞cdna文庫中克隆了pta1cdna全長，證實pta1是一個新分子，屬于免疫球蛋白超家族，胞膜外區有兩個v樣結構域。
  6. According to medical research, when vitamin b12 enters the body, it forms a compound with an intrinsic factor secreted by the parietal cells large cells of the peptic glands on the gastric mucosa mucous membrane of the stomach, before being absorbed by receptors in the ileum lower part of the small intestine in the presence of calcium ions

    在維他命b12的吸收方面，根據醫學報導，維他命b12在吃進人體后，首先會在胃部，和胃壁細胞parietal cells分泌的一種蛋白質內因子intrinsic factor結合，形成復合物后，再由小腸段中的回腸ileum吸收。在回腸之接受體receptors吸收時，需要有鈣離子之存在。
  7. 二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system

    二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳，利用tanongis凝膠圖像處理系統分析其含量。
  8. In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation

    而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料，分別用高鈣溶液、生理溶液、低鈣溶液孵育后，通過sds ? page電泳技術及免疫膠體金電鏡技術，研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。
  9. Purification and characterization of a protein from pilose antler and its proliferative effects on mdck cells

    鹿茸中促腎上皮細胞增殖蛋白的分離純化與表徵
  10. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  11. The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell, collegenase i ( 150iu ml - 1 " ) and trypsin ( 0. 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue, which is appropriate to obtain qinchuan - scalper skin cells

    組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養物。其中150iu ? ml ~ （ - 1 ）膠原酶i與0 . 05胰蛋白酶（ 1 ： 1 ）同時應用能更好的分離秦川牛皮膚組織，是獲得秦川牛皮膚細胞的適宜方法。
  12. Studies revealed that p - catenin dissociated from ccc and translocated into free catenin pool in cytosol after it has been phosphorylated at tyrosine or serine residues, and in this situation, the ccc has been disrupted and cell adhesion function disturbed. a large amount of the free p - catenins in the cytosol can be degraded by the tumor suppressor apc, and the remains translocate into nucleus and bind to transcriptional factor tcf / lef in the nucleus and then promote cell proliferation related gene or anti - apoptosis gene transcription

    當-連環蛋白酪氨酸或絲氨酸殘基磷酸化后，就與ajs發生解離而游離到細胞漿中，此時細胞的粘附功能也發生障礙，游離到胞漿中的-連環蛋白，一部分被抑癌因子apc降解，一部分則轉移到細胞核內，與核內的轉錄因子tcf lef結合，啟動與細胞增殖有關的基因轉錄。
  13. During the culture of the porcine ear skin fibroblasts, the successful rate was increased by selecting culture methods, contrasting donor age and improving culture condition. the rate of living cells in the method of combining trypsin cold treatment with trypsin heat treatment was much higher than that in the method of trypsin heat treatment. the results showed that the method of combining trypsin cold treatment with trypsin heat treatment had less adverse effects on cells than the method of trypsin heat treatment

    在培養豬耳皮膚成纖維細胞時從篩選細胞分離方法、比較供體年齡和改善培養條件這三方面著手提高培養的成功率。實驗中發現胰蛋白酶冷熱處理結合法培養的細胞存活率明顯高於胰蛋白酶熱處理法，說明使用胰蛋白酶冷熱處理結合法對細胞的不利影響較少，細胞產量較高。
  14. Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively

    Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
  15. Ddl5mc widely used in the field of pharmacy, biologic product and central blood station

    Ddl5mc適用於血站、生物化學、生物製品、制藥行業使用，進行血液分離，蛋白沉澱，細胞分離收集。
  16. Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells. only after adhering to surface followed by spreading can cells develop and proliferate. most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity, which may correlates to the low degree of cell attachment. modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation. cell attachment to substrate is primarily mediated by integrins, a widely expressed family of heterodimeric surface receptors. most extrcellular matrix proteins such as fibronectin, osteopontin, collagen type i, bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin. the main aim of this research is to measure, assess adhesion, proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin, collagen type i or biotie gen, which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture. ( 2 ) adhesion, proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed. ( 3 ) also, adhesion, proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin, or collagen type i respectively. ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo. this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities

    因此將這些蛋白包被、固定到材料表面，觀察骨組織工程種子細胞mscs細胞的粘附、生長特性是本研究的中心環節，並從以下方面進行探討： （ 1 ）採用不同原代細胞分離方法，研究其對mscs細胞的生物學特性影響。 （ 2 ）檢測基因勝肽膠對mscs細胞粘附、增殖及分化的影響。 （ 3 ）分別採用型膠原及纖維粘連蛋白（ fibronectin ， fn ）包被聚乙醇酸-乳酸共聚物（ poly （ 1actide - co - glycolide ） ， plga ）膜及多孔塊型plga材料，觀察細胞在單層或三維培養狀態下，型膠原及fn對mscs細胞粘附、增殖及向成骨細胞分化效應及能力。
  17. One small captive population of amur tigers ( panthera tigris altica ), which had 14 - year captive history since its foundation in 1986, was selected for such population genetic researches in this paper, which contained three experimental parts as : ( 1 ) dna extraction from shed hairs. three methods, which were chelex, ionic detergent and non - ionic detergent, were respectively used to digest the shed hairs of one amur tiger

    本文利用chelex 、離子型和無離子型分解液三種方法對在東北虎（ pantheratigrtsaltica ）換毛期採集的自然脫落毛發進行了dna提取，並對部分毛發分解原液進行蛋白質沉澱，對于毛發分解殘留物也進行了核細胞分解與蛋白質沉澱再處理，對上述提取產物進行rapd擴增。
  18. These proteins involved in various cellular functions including carbohydrate transport and metabolism, energy production and conversion, inorganic ion transport and metabolism, amino acid transport and metabolism, posttranslational modification, protein turnover, chaperones, function unknown and not in cog most of the proteins identified are involved in energy production and conversion, carbohydrate and amino acid transport and metabolism, especially in energy production and conversion

    這些蛋白質在細胞中行使不同的功能，主要包括: 1 .糖類轉運與代謝: 2 .能量的產生與轉化; 3 .無機離子轉運與代謝: 4 .氨基酸轉運與代謝; 5 .翻譯后修飾，蛋白代謝，分子伴侶: 6 .一些未知功能的蛋白等。在鑒定的13個蛋白中大多數是與糖類和氨基酸轉運及代謝和能量的產生與轉化相關。
  19. Some trends in the studies of cellular inorganic chemistry have been described in this review, which mainly contains the aspects of effects of metal ions including calcium ions and lanthanide ions and activated small molecule on ion channels, receptor proteins in the membrane, intracellular receptor proteins and cellular function

    摘要本文評述了當前細胞無機化學研究中的幾個方面，主要是包括鈣離子和稀土離子在內的金屬離子和活性小分子對細胞膜離子通道、膜受體蛋白、胞內受體蛋白以及對細胞功能的影響。
  20. Results : the neural stem cells isolated in newborn rats had the potential of forming clones and serial passage, expressing neuroepithelial stem cell protein ( nestin ) and differentiating into neurons and astrocytes and oligodendrocytes

    結果：新生大鼠腦組織分離的細胞具有連續傳代和增殖能力，表達神經上皮幹細胞蛋白，誘導分化后的細胞表達神經元細胞、星形膠質細胞、少突膠質細胞特異性蛋白。
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