蛋白結合能量 的英文怎麼說

中文拼音 [dànbáijiēgěnéngliáng]
蛋白結合能量 英文
protein binding capacity
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 結 : 結動詞（長出果實或種子） bear （fruit）; form （seed）
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 能 : 能名詞（姓氏） a surname
  • 量 : 量動1. （度量） measure 2. （估量） estimate; size up
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  • 結合 : 1 （發生密切聯系; 聯合） combine; unite; integrate; link; binding; coalition; cohesion; connectio...
  • 能量 : 1 [物理學] energy; amount of energy 2 （能力） capabilities; capacity; 能量不滅 conservation of e...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  3. Besides, 5 % methanol enhanced the resistance to freezing stress in the sensitive species, and this effect seems to be related to maintenance of the psi activity and energy transfer between pigment protein complexes by methanol

    值得注意的是5甲醇提高了非抗凍型品種synechocystissp . pcc6803的凍結耐性。其作用機制可能是在凍結脅迫下保持ps活性和色素蛋白復合體之間的能量傳遞。
  4. In aqueous solution, interaction between crude venom and different bacteria including gram - positive bacteria, i. e. streptococcus mutans, streptococcus aureus, and gram - negative bacteria, i. e. e. colik12, was investigated in detail. molecule weights of multiple proteins were identified as 110kda, 47kda, 24kda, 23kda and 15kda by sds - page, respectively. it had little influence on extraction of snake venom proteins by the same cells of streptococcus mutans even used for 10 cycles

    其次，在利用s . mutans分離細菌特異性抗體的基礎上，首次利用細菌篩選作用蛋白，將革蘭氏陽性菌（ s . mutans和s . aureus ）和革蘭氏陰性菌（ e . colik12 ）分別與蘄蛇蛇毒相互作用，發現有五種蛇毒蛋白能夠與細菌相互作用，其分子量分別為110kda 、 47kda 、 24kda 、 23kda和15kda ；同時表明s . mutans菌體能夠多次與蛇毒相互作用，並且對作用蛋白的結合沒有影響。
  5. And it easily degraded. so it ' s difficult to isolate and purify rbp from human and animal plasma and urine of kidney patients. in order to study the structure - function of rbp, we produced rbp from escherichia coli by constructing engineering strain which can express rbp

    維生素a結合蛋白在體內的含量很低，容易降解，從人或動物的血漿以及腎病綜合征患者的尿中分離提取有活性的維生素a結合蛋白十分困難，極大的限制了對其結構、功能及代謝機制的研究，而且該蛋白價格昂貴，也不利於臨床的廣泛應用。
  6. 2. the molecular weights of the recombinant protein of the cadherin repeated domain 4, 5, 6 of bt - r3 were about 42kda and 45kda ( with his tag ) respectively. both expressed products could not specifically bind to bt toxic protein crylab

    表達得到了bt - r3受體的第4 ， 5 ， 6個鈣粘蛋白結構域重組蛋白，其分子量約為42kda和45kda （帶histag ） ，均不能有效地結合bt毒蛋白cry1ab 。
  7. Quantitatively describing the principle of protein - protein interactions and recognition in molecular level is the key point for understanding the relationship between structure and function of protein complexes and designing protein complexes, hi these interactions among proteins, electrostatic and hydrophobic interactions are the two most important ones, which let protein molecules bond systematically

    在分子水平上定量地闡明蛋白質分子之間的親合力和相互識別的機制，是正確地認識蛋白質聚集體結構和功能之間的關系併合理地設計出有用的蛋白質分子聚集體的關鍵。蛋白質分子間相互作用，其中最主要的是靜電和疏水相互作用，使蛋白質分子能有序地結合在一起。
  8. The protein level of cyclin e increased lightly after 2gy, but it increased from 1 to 24 h after logy, level of cyclin e associated with cdk2 increased in response to logy, at the same time, there was no significant changes in the level of cdk2, while cdk2 levels remained constant in hela cells. our results indicated that the kinetics of cyclin e / cdk2 kinase activity reflected the increase in cyclin e expression levels

    Cycline蛋白表達水平在zgy照射后略有增加， 1ogy照射后lh開始增加，持續到24h ，但ir后cdkz蛋白水平未發生變化，通過免疫共沉澱檢測了10gy照射后edkz與cydine結合，發現隨著照射時間的延長， cycline與cdkz結合量增多，這說明cycline蛋白水平的增加能反映cycline / cdkz的激酶活性。
  9. We have demonstrated isolation of single - class plasma lipoproteins based on the immunological method associated with streptavidin ? biotin capture technology, and the lipoprotein cholesterol was consecutively quantified on the same strip without handling of reagents ( 11 )

    我們還論證了基於免疫學方法結合鏈親和素捕獲技術分離單一級別血漿脂蛋白，並且在同一條帶上不用對反應物進行操作就能連續定量檢測脂蛋白膽固醇。
  10. We can draw a conclusion that the mixture can promote psb reproduction and protein content

    實驗結果表明，氣態混合物能夠促進光合細菌的生長，菌體蛋白含量明顯增加。
  11. Nonspecific lipid transfer proteins ( nsltps ) are small, soluble, basic proteins that can bind and catalyze transfer of lipids in vitro, however, we do not know their functions in vivo

    非特異性脂轉運蛋白是一類分子量較小的可溶性堿性蛋白質，許多研究表明在體外它能結合併跨膜運輸磷脂、糖脂和脂肪酸等物質。
  12. The stage when the block occurs has been correlated to the time the embryonic genome takes over control of development from the maternal genome, which occurs in mouse at the 2 - cell stage. the embryos of balb / cxicr fl mouse show 2 - cell block in vitro and the effects of several culture media on overcoming the 2 - cell block in embryos development were studied. m16 ( sigma ), in which 96. 2 % of 1 - cell embryos developed to 4 - cell stage, were shown to be effective in overcoming the 2 - cell block

    培養5h后， 53去核卵母細胞可見標記的mtocs ，但數量明顯少於對照組，而又多於培養0h的去核細胞，說明此時53的化學去核卵胞質中確有mtocs的存在，其微管聚合能力正在恢復； mtocs標記，但其輻射產生的微管纖維短，結構還不夠豐滿；其餘細胞的微管蛋白則喪失重新裝配能力。
  13. Built with dna or protein molecules, these nanoelectronic devices are suitable for many applications in life science and biomedicine, and have achieved better performance for bio sensors and chemical sensors

    結合dna與蛋白分子，納米電子裝置可以大量應用於有關生物科技和醫療的用途上，並能提高生物和化學探察器的功能。
  14. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  15. Huctla4 - lg, " one of the most potent in1n1unosuppressive molecules, is a soluble recombinant fusion protein ( molecu1ar weight of approximately 92 kd ) consisting of the extracellular domain of human ctla - 4 and a fragment ( hinge and constant region ) of the fc portion of human iggl. it strongly adheres to the b7 molecule to block the cd28 - mediated costimulatory signal, resulting in inhibition of in vitro and in vivo immune response

    可溶性重組融合蛋白huctla4 - ig是一種新穎的免疫抑制劑，分子量約為92kd ，由人ctla - 4分子的胞膜外區與人igg1的fc段（鉸鏈區與恆定區）融合形成，能牢固地與b7分子結合，阻斷cd28介導的共刺激信號，導致免疫反應的抑制。
  16. We integrated dh gene into procaryotic expression vector for the first time, which establish foundation for obtaining dh recombinant protein to research the structure and function of dh

    本研究首次將dh基因整合入原核表達載體中表達，為大量獲取dh重組蛋白，研究dh的結構及功能奠定了基礎。
  17. Objective 1. to study the structure and function of the membrane penetrating peptides and found new peptide vectors. 2. to observe the intercellular transport property of a novel synthesized peptide. 3

    方法利用蛋白質分析軟體peptoollife對人量膜穿透肽序列進行系統分析，掌握其空間結構及理化性質特點，並結合各種氨基酸的結構、性質特徵，設計合成具有更強穿透能力的膜穿透肽類似物。
  18. Sds - page proved that the molecular weight of expressed product of the extracellular part of bt - r3 was about 160kda. western blotting showed that the recombinant protein could specifically bind to bt toxic protein crylab, indicating that the bt - r3 is a receptor protein of crylab. the binding site of bt - r3 with crylab was located in extracellular domains of bt - r3 protein

    用大腸桿菌系統表達的bt - r3基因的胞外結構域的分子量約為160kda ， western - blot實驗證實，重組蛋白能有效地結合bt毒蛋白cry1ab ，從而進一步確證了bt - r3是cry1ab的受體基因。
  19. Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage

    構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。
  20. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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