蛋白菌 的英文怎麼說
中文拼音 [dànbáijūn]
蛋白菌
英文
delta proteobacteria-
After the expression form analysis, the insoluble recombinant proteins was purified by destraction and abstersion of inclusion bodies. to study the abstersion condition of the inclusion bodies, we adopted ultrasound crushing and freezing - melting methods
採用超聲加洗滌液破碎菌體；離心加凍融分離純化融合蛋白，研究不同的超聲次數和凍融對包涵體洗滌效果的影響。Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Daqu, a microbial product containing multiple enzymes and bacterial species, its aroma - producing substances mainly come from proteins and fat of daqu - making materials and the degradation of amylum and those substances are composed of amino acids, fatty acid, polyose and its polymers etc
摘要大?是一種富含多酶多菌的微生態製品，大?復合曲香物質來源於制曲原料中的蛋白質、脂肪以及澱粉等的降解，其復合曲香由氨基酸、脂肪酸、多糖及其聚合物等多種物質共同構成。Overexpression of sweet potato feathery mottle virus coat protein in e. coli and preparation of its specific antiserum
甘薯羽狀斑駁病毒外殼蛋白基因在大腸桿菌中的表達及特異抗血清的制備Most of plants, when attacked by avirulent pathogens, usually produce a hypersensitive response ( hr ) at the locus attacked and develop a systemic acquired resistance ( sar ) throughout entire plant by expressing a set of defense genes including pathogenesis - related ( pr ) genes
植物受到病原菌侵染后，感染部位產生過敏性壞死反應，在其它部位產生系統獲得性抗性並伴隨抗性基因包括病程相關蛋白（ pathogen - relatedprotein ）基因的表達。By using the similar separation and purification methods, a nifh and a nife mofe protein were obtained from azotobacter vinelandii mutants dj54 and dj35. the results of the complement activity experiments showed that a nifh mofe could be activated by femoco
從棕色固氮菌突變種dj _ （ 54 ）中得到了nifhmofe蛋白；並參與了棕色固氮菌突變種dj _ （ 35 ）的nifemofe蛋白的分離純化，所用方法與nifb ~ - mofe蛋白的分離純化相似。In bacterial systems transport activities are performed by membrane proteins.
在細菌系統中運輸活性是通過膜蛋白實現的。Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da
採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱，通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰，這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的，通過tricine - sds - page檢測，只能檢測到一條分子量相對較大的細菌素，分子量在8 ， 570da左右。Now, in a study that took more than five years to complete, rockefeller university researchers, in collaboration with a team of bacteriologists at the university of wisconsin, madison, have become the first to solve the structure of a protein complex that protects these cells from singlet oxygen
現在，洛克菲勒大學的研究人員與威斯康星大學的細菌學家一同協作，歷經5年多時間的研究，首次獲得了一種能夠保護細胞免受單態氧損傷的蛋白復合體的晶體結構。The leghaemoglobin surrounding the bacteroids serves a dual role.
類菌體四周的豆血紅蛋白執行雙重職能。The results showed that : adding tryptone, soy peptone. beef extract, com extract and cys - hcl to jaj could obviously promote the growth of blm and bbm ; by the orthogonal experiment of three elements and three levels, a satisfying jaj compound medium was acquired which included corn extract ( 0. 3 % ), soy peptone ( 0. 05 % ) and cys - hcl ( 0. 025 % ). nextly, after establishing a selective bifidobacterium medium, the effects of jaj on the growth of bifidobacteria in vivo were studied, using healthy mouse of kunming species as experimental animal
研究了以菊芋汁為主要原料的雙歧桿菌培養基，大量試驗結果表明，在菊芋汁中添加胰蛋白腖、牛肉膏、大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽等成分，對雙歧桿菌有明顯的促進生長作用；利用大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽設計了三因素三水平的正交試驗，確定了菊芋汁復合培養基的優化配方：菊芋汁+ 0 . 3玉米漿+ 0 . 05大豆蛋白腖+ 0 . 025半胱氨酸鹽酸鹽。Isolate all grew well in the culture medium with initial ph 4 - 10, the optimal growth temperature range was from 28 to 30. 5 degree c. it grew well on the medium for fungi growth, such as pda medium and czpek medium etc, and also grew well on the cause ' s i medium and the non - nitrogen medium, but little growth on the luria bertani medium ( lb ) and beef extract peptone medium. it did not need special nutrition factors for growth, but source of the carbon was the key factor to growth, all of its nutrition needs were different from that of common bacteria
該菌在初始ph4 - 10的培養基中都能夠生長，生長最適溫度范圍為28 - 30 . 5 ，在pda 、查氏等真菌培養基中生長旺盛，在高氏1號和無氮源培養基中同樣生長良好，而在lb與牛肉膏蛋白腖等細菌培養基中生長很差，碳源是其生長的關鍵因子，這有別於一般細菌的營養需求。Seed culture conditions of bacillus licheniformis ts - 01 were firstly investigated. the results showed that the optimal growth temperature was 40 c ; the suitable seed culture medium was beef extract soya peptone medium ; the optimal initial ph was 7. 5 ; the optimal seed culture time was 13h ~ 14h
首先對地衣芽孢桿菌ts - 01的種子培養條件進行了研究，得到的結果為：最適生長溫度為40 ；較適合的種子培養基為牛肉膏大豆蛋白腖培養基；最適初始ph值為7 . 5 ；種齡為13h 14h 。We screened the resulting e. coli samples using blood serum from a person ( me
的血清，來篩檢大腸桿菌產生的蛋白質樣本。They identified two bacteria, lactobacillus casei and lactobacillus plantarum, which can be added to beans so they cause minimal distress to those who eat them, and to those around the bean - lovers, marisela granito of simon bolivar university in caracas, venezuela and colleagues reported
研究人員發現，把兩種細菌酪蛋白乳酸桿菌lactobacillus casei和植物乳酸桿菌lactobacillus plantarum添加到豆子中，就可以讓其產生氣體的可能性變小。這樣豆子愛好者們和他們身邊的人就都能從中受益。It was firstly reported in china. the third part rthe capsid protein vp | gene was expressed. 810 bp fragment was inserted into vector pgex - 4tl, and then transformed into e. coli bl21
這是國內首次對aev基因組全序列的分析；第三部分： aev外殼蛋白vp _ 1基因在大腸桿菌中的融合表達、純化及活性研究。Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted
因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明， arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝： （ 1 ） arg可能影響胞外蛋白酶的活性，進而促進含氮大分子物質的分解代謝，補充發酵過程中的氮素來源。After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including estss analogous to 11. 5kd antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library
在仔細分析了它們在免疫系統中的重要性和在對蝦中出現的可能性之後，從中選出了17條可能編碼抗菌肽，溶菌酶，凝集素、絲氨酸蛋白酶及其抑制劑，抗凍蛋白等蛋白質的序列，以此為依據設計引物，在中國對蝦的血液和頭胸部cdna文庫中擴增相應的序列。Conclusion the concentrations of cefoperazone sodium, chloromycetin, ciprofloxacin lactate and gentamycin sulfate showed significant influence on the coagulation time and detection rate of fibrinogen in plasma
結論頭孢哌酮鈉、氯黴素、乳酸環丙沙星、硫酸慶大黴素等抗菌素及其濃度變化對血漿中纖維蛋白原的凝固時間和檢出率有顯著影響。分享友人