融合表達載體 的英文怎麼說

中文拼音 [rónggěbiǎodázǎitǐ]
融合表達載體 英文
fusion expression vector
  • 融 : Ⅰ動詞1 （融化） melt; thaw 2 （融合; 調和） blend; fuse; be in harmony Ⅱ形容詞[書面語]1 （長遠; ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 融合 : fuse; mix together; anastomosing; reconcile; harmonize; compromise; amalgamate; coalesce; coalesc...
  • 表達 : deliver; express; show; voice; convey; communicate
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  2. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. It was established that grb - ast3 concatemer are more easily expressed in the way of fusion expession

    初步認為grb - ast _ 3串聯體基因適宜採用融合表達載體表達。
  5. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  6. It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed

    的序列正確，經酶切鑒定證實成功地構建了綠色熒光蛋白基因融合表達載體。
  7. By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature

    利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。
  8. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  9. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  10. Construction and expression of recombinant eukaryotic expression plasmid of ubiquitin and epstein - barr virus nuclear antigen 1 fusion gene

    病毒核抗原1的融合表達載體的構建及表達
  11. 1 ( + ) / hsblys was trans fected into cos - 7 cells with the calcium phosphate method and the lipofection method. we cultured the recombined cos - 7 cells at optimal condition and harvested cells every 24h. in order to find out the optimal expressive condition, we lysed the cells, extracted the protein, and measure the content of the hsblys

    此外，用構建的融合表達載體pcdna3 . 1 （ + ） hsblys轉染真核宿主細胞cos - 7 ，然後在合適的培養條件下進行表達，取不同表達時間（ 48h 、 72h和96h ）的細胞破碎后進行分析鑒定，找出最合適的表達時間及其他條件。
  12. Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ), then subcloned into the eucaryote expression vector pcdna3. 1 ( + ) after sequence analysis

    將halr基因克隆到原核融合表達載體pet28a （ + ）上，序列分析后亞克隆到真核表達載體pcdan3 . 1 （ + ）上。
  13. In this study, vp7 gene and ctb - vp7 fusion gene expression vectors were constructed, and a high - efficient genetic transfomation system of carrot ( daucus carota l. ) was established. vp7 gene was amplified from cdna of rotavirus antigene by pcr and was inserted into expression vector pbi121 containing no gus gene

    本實驗以輪狀病毒的vp7為目的基因，構建了vp7基因的表達載體和vp7 - ctb融合表達載體；以胡蘿卜為受體材料，建立並優化了胡蘿卜的轉化體系，獲得了能正常轉錄vp7基因的轉基因胡蘿卜植株。
  14. So, we subcloned emt - 1 gene into the prokaryotic fusion expression vector prsetb and generated a 24ku protein

    為此將emt l氨基末端基因克隆入融合表達載體prsetb中，轉化大腸桿菌bl 21 ，經iptg誘導，表達his6 emt l融合蛋白，表達產物分子量約為24ku 。
  15. The total rna was extracted from human normal kidney tissue and the cdna fragment of hnadcs was produced by rt - pcr, then was purified and inserted into pgem - t vector. after dna sequenc - ing, pgem - hnadcs was digested with ecor i and sal i, and ? the dma fragment was subcloned into the ecor i and sal i sites of the fusion expression vector ( pgex - 5x - 1 )

    從正常人腎組織中提取總rna ， rtpcr擴增hnadc3抗原表位區cdna片段，產物克隆至pgem嚇載體，測序正確后，將hnadc3抗原表位區dna片段再次亞克隆至pgex融合表達載體，構建重組質粒pgex十nadc3 。
  16. The orf of the hbrp coding a peptide of 233 aa, using the dnasis v2. 5 demo analysis its structure and pi 3. screening 1 ) screening using restriction enzyme

    Pcr產物和pgex一sx一1融合表達載體分別用bamhi和x五01限制性內切酶雙酶切后連接，構建融合表達載體。
  17. The results indicated that : ( 1 ) marinated in 1 % agno3 for 15min was the optimal sterile condition of seeds. ( 2 ) 2, 4 - d had great effects on the formation of callus, and the optimal concentration was 0. 1mg / l

    在pbi121vp7表達載體成功構建的基礎上，利用質粒pbi121vp7和質粒puc19ctb上相同的ndei和xbai單限制性酶切位點，構建了pbi121ctbvp7融合表達載體。
  18. The l - scfv genes were inserted into the eukaryotic fusion protein expression vector pegfp - n3 and transfected transiently into cos - 7 cells to express respectively

    將所構建的單鏈抗體基因克隆入綠色熒光蛋白融合表達載體pegfp - n3 ，瞬時轉染cos - 7細胞，分析其表達情況。
  19. Gst - fusion and native rip from gp & nlaphyuum were expressed in e. coli by inserting the gp609 and rhxjb into the pgex - 2t vector, and dna - 8001 into the pet - 5a vector, respectively

    分別將gp609和側出口b轉入pgex一zt融合表達載體，在大腸桿菌dhs 。菌株中實現融合表達。
  20. The recombinant fusion protein was highly expressed in e. coli bl21 induced by immol / l iptg in the form of inclusion bodies. the molecular weights of gst - h a1 is 62kd

    將構建好的融合表達載體，在1mmol l的iptg誘導下，在大腸桿菌bl21中得到大量表達，經過4h表達量既達到高峰。
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