The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基
化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基
化蛋白。通過對畢赤酵母重組表達
的木聚糖酶xynba 、脫糖基
化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質
的比較發現:三種酶
的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba
的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基
化作用熱穩定性明顯高於未糖基
化的xynb和xynbb ; xynba和xynbb
的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶
的比活2814 . 45iu mg ; xynb和xynba
的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb
的km值較大為27 . 10 ( g kg ) ; xynba和xynbb
的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb
的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好
的抗性,且對作用環境中
的各種離子、表面活性劑、
螯合劑不敏感。通過對不同木聚糖
的酶解產物
的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79
的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。
The change of ferrous oxidation rate of ferrous glycinate in fortified flour was studied by storage test and the oxidation stability of ferrous glycinate was significantly better than that of ferrous sulfate
摘要通過貯藏試驗,研究了甘氨酸
螯合鐵在營養強
化麵粉中亞鐵氧
化率
的變
化,甘氨酸
螯合鐵
的氧
化穩定性明顯好於硫酸亞鐵。
Meanwhile, the change of ferrous oxidation rate of ferrous glycinate and ferrous sulfate after processed to flour products was compared respectively, and ferrous oxidation rate of ferrous sulfate group was obviously higher than that of ferrous glycinate group
同時比較了甘氨酸
螯合鐵和硫酸亞鐵在加工製作成各類面製品后
的亞鐵氧
化率
的變
化,硫酸亞鐵組
的亞鐵氧
化率明顯高於甘氨酸
螯合鐵組。
Rearrangements have been occurred in at least twelve mitochondrial genes, which have drastically altered the gene order of the mitten crab e. japonica sinensis to the putative ancestral pancrustacean ( crustacean / insecta ) gene order
中華絨
螯蟹線粒體基因組與推測
的泛甲殼類原始
的線粒體基因順序相比,至少有12個基因發生了重排,造成線粒體基因排列順序顯著
的變
化。
Effects of soy protein concentrate as a dietary protein source on digestive enzyme activities of eriocheir sinensis
配餌中不同大豆濃縮蛋白含量對中華絨
螯蟹消
化酶活力
的影響
The gel by sodium alcoholate method, added benzoylacetone, have the obvious uv absorption peaks at 265nm and 337nm respectively. when the gel was placed in air, the chelate did n ' t have change. the absorption peak decreased until disappeared when the films were irradiated by the ultraviolet light with the relevant wavelength, which indicated that the films were obviously photosensitive
( 4 )通過三種方法制備感光性
的sno _ 2 : sb ( ato )溶膠,採用醇鈉法,添加苯酰丙酮制備
的二氧
化錫凝膠膜,在265nm和337nm處存在明顯吸收峰,空氣中放置,所形成
的螯合物不發生變
化,用相應波長
的紫外光照射時,這個吸收峰會減小直至消失,表明薄膜具有良好
的感光性。
Edta ethylenediamine tetra - acetic acid, a compound that acts as a chelating agent, reversibly binding iron, magnesium, and other positive ions
學名為乙二胺四乙酸,是一種具有
螯合作用
的化合物,能夠可逆地結合鐵離子、鎂離子以及其他正離子。
Both the frequency and intensity of 1146. 0cm - 1 change largely, etc. discussed and compared the mechanism of these interaction in detail we got some conclusions as following : both zn2 + and cd2 + ions can chelate with po2 - and come into being - po2 - me2 + n7 ( purine ) chelate. as to zn2 + ions, when r2. 0, the quantity of po2 - zn2 + n7 is directly linear with r. when r2. 0, zn2 + ions even can chelate with c at n3 - o2 sites and this chelation will disrupt the h - bond between gc base pairs and make the dna structure unstable. as to cd2 + ions, only when r is within 1. 0 - 1. 5 can this chelation take place and the relation of the quantity of this chelate with r is not easy to conclude
通過對這些變
化進行詳細討論和分析,我們得到了如下
的結論: zn ~ ( 2 + )和cd ~ ( 2 + )都可以跟dna
的磷酸基團
螯合,形成- po _ 2 ~ - … me ~ ( 2 + ) … n7 ( g )
螯合物,但是前者
的螯合跟離子濃度有很大
的關系,在摩爾濃度比小於2 . 0時,
螯合物
的生成量跟r成正比關系,而後者
的螯合跟離子
的關系不是很明顯,只有當r處於1 . 0至1 . 5之間時,
螯合作用才能進行,並且
螯合物
的生成量跟r之間沒有很明顯
的關系存在。
These can include animal - based, cell culture - based, biochemical, or ligand / receptor - binding assays
這些分析方法可以包括基於動物
的,細胞培養
的,生物
化學
的或配位體/接受體
螯合
的分析方法。
Gonadal development and fecundity are affected by certain essential dietary nutrients, especially essential amino acids, essential fatty acids, and vitamins etc. thus, during the last two decades, more attention has been paid to the optimal level of different nutrients in broodstock diets. it is well known that protein is a kind of indispensable nutrient because of its important physiological function on body structure and supplying energy to the growth and development of shrimp and crab. protein and amino acids of broodstock diet have been identified as major dietary factors that determine successful reproduction and survival of offspring
而這些問題
的解決則要求對中華絨
螯蟹生殖過程中
的攝食和消
化生理機制,親體性成熟期對各種必需營養素(如必需氨基酸、必需脂肪酸和維生素等)
的需求,卵細胞發生過程中營養物質
的合成、轉運及貯存規律,以及在受精卵、胚胎和后續以內源性營養為主
的?狀幼體發育階段對卵黃磷蛋白
的消耗和利用模式等環節進行較為深入
的研究,在全面系統地了解中華絨
螯蟹
的生殖營養生理
的基礎上,探討親體生殖營養對卵質、孵
化及后續幼體生長發育
的影響。
This paper reviews the effects of al on photosynthesis, photoprotective systems, water use efficiency ( wue ), water content, carbohydrate content, mineral nutrition, organic acids, and nitrogen metabolism in plant shoot, and also the mechanisms of al detoxification ( chelation of al with small organic compounds, and isolating al in the some parts, which are insensitive to al, such as vacuoles or epidermal cells ) by al accumulators
為此,本文綜述了鋁對植物地上部光合作用、光保護系統、水分利用效率、含水量、碳水
化合物含量、礦質營養、有機酸和氮代謝
的影響,並對富鋁植物
的解鋁毒機制(鋁與小分子有機酸
螯合和把鋁隔離在對鋁不敏感
的表皮細胞和液泡內)進行了綜述。
Sepharose affinity chromatography. the apparent molecular weight of purified ghrp - scu - pa - 32k was 33kd by sds - page and mass spectrometry and its specific activity was 150000iu mg protein, according to fibrin plate determination
經鋅離子
螯合親和柱純
化的產物, sds - page顯示為單一蛋白條帶,分子量為33kd ,質譜法測定分子量也為33kd 。
The amount of the goal protein was evaluated by densitometric scanning. it indicated that the 45ku product of the vp6 gene was 26. 5 % of total bacterial protein of bl21
表達產物經ni - nta金屬螯合層析柱純化后,得到純化的his - taggedvp6融合蛋白。