After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載
體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛
蟲感染兔血清,成
蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛
蟲單抗特異識別。
The main conclusions include : 1 ) among the non - biological factors, temperature is the most important factor that influence the development of the cochineal insect, the insect cannot finish life cycle under 15 associated conditions in spite of a few of egg can hatch, while at associated conditions of temperatures 20, 25, 30, the insect can finish life cycle, but comparing from the amount of eggs, generation tendency indexes and the sizes of the female adults, 25 associated conditions are the best
得出的主要結論包括: 1 )在胭脂
蟲的生長發育過程中,溫度是影響胭脂
蟲發育的最主要因子,在15的恆溫狀態下,盡管胭脂
蟲卵有少量孵化,但不能完成世代,而在20 、 25 、 30的恆溫下胭脂
蟲能正常發育並完成世代。從培育的胭脂
蟲雌
蟲體大小、懷卵量及世代傾向等方面比較, 25的溫度最有利於胭脂
蟲的培育。
In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system, respectively. in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus. a preliminary bioassay was conducted
第五章利用桿狀病毒bac - to - bac系統構建了含有油桐尺蠖核多角
體病毒的類蝸牛毒素基因的重組病毒racbacctl和rhabacctl ,在其相應宿主甜菜夜蛾和棉鈴
蟲的細胞水平和
蟲體水平進行了超表達實驗。
Nutrient and some chemical substance of teleogryllus derelictus gorochov have been measured from 1 instars, 3 instars, 5 instars, 7 instars, 9 instars, adult of 3 days after emergence and adult of 10 days after emergence. the result as follows : the average content of protein of the different growth stages of teleogryllus derelictus gorochov amounts to 15. 92 %
試驗根據黃褐油葫蘆的個
體發育過程,選取卵、 1齡、 3齡、 5齡、 7齡、 9齡若
蟲、羽化3日的雌、雄成
蟲和羽化10日的雌、雄成
蟲10個發育階段,分別測定其
蟲體所含的營養成分和化學物質。
It was not until a scalp scraping showed numerous sarcoptes mites and ova that the diagnosis of keratotic scabies was finally made
直到頭部皮屑檢查發現許多疥
蟲蟲體與
蟲卵,才確診為角化型疥瘡。
This rare ocular angiostrongyliasis was received supportive treatment and close observation only
經過三個月的觀察及治療,癥狀改善,左眼
蟲體融解后吸收。
Isolation and activity determination of somatic cells from trichinella spiralis larvae
旋毛形線
蟲體細胞的分離及活性檢測
The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明:麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白,分子量分別為181kd和136kd ; sds - page電泳分析:存在3條雌特異性帶,其分子量為123kd 、 120kd和91kd ,由此,可推定卵黃原蛋白( vitellogenin , vg )和卵黃磷蛋白( vitellin , vn )由2個蛋白組成,其中分子量較大的蛋白由2個亞基組成;雙向電泳結果顯示,在120kd附近有兩個特異性點,其等電點為5 . 5和5 . 7 ;雙擴散表明,麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應,而與雄蜂血淋巴無免疫反應,說明了vg與vn具有免疫同源性,是雌特異性蛋白,且由脂肪體合成。