血培養基 的英文怎麼說
中文拼音 [xiěpéiyǎngjī]
血培養基
英文
blood culture medium-
Chalk blood yeast dextrose litmus milk
白堊血酵母浸膏石蕊牛乳培養基The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。Medical microbiology - culture media - part 2 : ready - to - use blood culture systems
醫學微生物學.培養基.第2部分:即可使用的血液培養設Microbiology of food and animal feeding stuffs. horizontal method for the enumeration of coagulase - positive staphylococci staphylococcus aureus and other species. part 2 : technique using rabbit plasma fibrinogen agar
食品和動物飼料微生物學.凝膠水平計數方法.陽性葡萄球菌和其它屬.第2部分:兔原生質血纖蛋白質瓊脂培養基技術Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently, setting up to express system efficiently, suspending culture cell, can raise the cell density in the scale turn the production, strengthen the cell vitality, control cell to propagate level, extension cell culture period, increase the target protein of yield, raise product quality, simplification of produces technics, reduce production cost, then raising the efficiency that the scale turns culture
提要：採用無血清無動物組分培養基馴化高效表達細胞，構建高效表達系統，懸浮培養細胞，可以在規模化生產中，提高細胞密度，增強細胞活力，控制細胞增殖水平，延長細胞培養周期，增加目標蛋白的產量，提高產品質量，簡化生產工藝，降低生產成本，進而提高規模化培養的效能。Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell
經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。Furthermore, the scientists were also able to grow progenitor blood cells in culture from uniparental es cells, and upon transplant into irradiated adult mice, show that these cells contribute, long - term, to the function of their hematopoietic system
此外，科學家還能夠利用單系胚胎幹細胞在培養基中培育血液祖細胞，在移植入輻射照射的成年小鼠后，發現這些細胞對造血系統功能產生長期的作用。4. rat - bovine interspecies somatic nuclear transfer 1 ) bovine cocs had been cultured in the maturation medium containing 8 - 10 % ocs in vitro for 22 - 24h, 60. 86 % - 61. 41 % of maturation oocytes can be obtained
（四）大鼠牛種間體細胞核移植研究的主要結果牛卵母細胞在含8 10發情牛血清（ ocs ）的成熟培養基培養22 24h ，能使60The cell - matrix complex was incubated with dmem and 10 % bovine serum under condition of 37, 5 % co2 - the medium was changed daily. ( 3 ) cell - matrix complex paraffin section was made after 7 days incubation
將細胞懸液接種于戊二醛交聯的膠原一殼聚糖多孔膜，加dmem 10小牛血清培養基後放置37 』 c ， 5 co 。Results isolation, culture and purification of the mmscs typically, mmscs were isolated from bone marrow by their adherence to plastic and grew as fibroblastic cells that developed into visible symmetric colonies
顯色15 30分鐘。結果1 mmscs分離、培養及純化用含10胎牛血清的dmem ? lg培養基在玻璃培養瓶中培養骨髓單個核細胞。Methods isolation, culture and purification of mouse mscs mmscs were isolated from the femurs of adult mice four weeks old and propagated in vitro. mmscs were originally cultured in dmem - lg with 10 % fbs, 100u / ml penicillion, 100mg / ml streptomycin
在細胞沉澱中加人含10胎牛血清的dmem lg培養基，計數細胞數，調整細胞密度，按3x10vinl接種於25cm 『的玻璃培養瓶中。Methods : the chromosome aberrations and the rate of micronuclei in peripheral lymphocytes were examined
方法：外周血淋巴細胞染色體畸變分析採用微量全血培養法，微核測定採用甲基纖維素法。The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively
主要內容和結果如下： （一）黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，採用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。The best condition for extracting polysaccharide from porphyridium cruentum were as follow : alcohol concentration was 50 %, alcohol volume was 1 - fold time, percolation time was 0. 5h, the volume ratio of glycoprotein solution to sevag reagent was 2 : 1, time was 45min and sevag reagent was 4 : 1 between chloroform and butanol. the result also indicate that sodium acetate anhydrous and nh4cl were the best carbonic and nitrogen source for polysa
血色紫球藻的最優提取工藝為乙醇濃度50 % ，乙醇用量為1倍體積，醇沉時間為0 . 5小時;氯仿與正丁醇的比例4 : 1 ，樣液與sevag試劑的比例2 : 1 ，作用時間為45min ;五種碳源的影響差異不顯著，氮源的影響差異顯著，其中添加無水乙酸鈉和nh4ci的培養基多糖產率最高，分別為33 . 784mg / l和40 . 997mg / l 。Cultured method of cord blood - derived mesenchymal cells in vitro and characters of culture medium
人臍血間充質細胞體外分離培養方法及培養基特性The results showed that : when cultured in the medium of m199, supplemented with 20 % bovine serum containing a moderate amount of antibiotics, the incubtion ph 7. 2 - 7. 4, the culture temperature 28. the primary culture cells formed the dense single wall within three weeks, the generation time of the subculture cells was 5 - 6 days, most cultured cells were fibroblastic - like cells with few epithelial - like cells
研究初步表明：在m199培養基加入20小牛血清（常規量雙抗） ， ph在7 . 2 - 7 . 4之間， 28的培養條件，四倍體鯽鯉魚腎組織原代培養三周左右即可形成緻密單層，傳代細胞為5 - 6天左右傳一次。培養細胞以成纖維樣細胞為主，有少數上皮樣細胞。This peptide is derived from proteolytic processing of the amyloid precursor protein ( app ). an increasing ' amount of experiments show that a p can induce various toxic reaction in brain, thus play a causal role in the pathogenesis of alzheimer ' s disease ( ad ). in the meantime, the incapability of neurons to regenerate in ad patients means that it may inhibit the proliferation, differentiation and even survival of nscs
3 .體外培養14天後，取部分細胞，加入不含bfgf僅含egf （ 20n留ml ）的無血清培養基，分為三組:對照組; ap25一35毒性組加入20umol /的凝聚態a目25一35 :雌激素保護組同時加入zoumolz的凝聚態ap25一35和10一7mo比17一p雌二醇。Development of insect cell culture media and serum - free media, establishment of insect cell lines and applications of insect cell culture for production of biopesticides and expression of recombinant proteins in genetic engineering were reviewed in this paper
摘要綜述了昆蟲細胞培養基的現狀和發展、無血清培養基的開發、昆蟲細胞系的建立以及昆蟲細胞在生物農藥和重組蛋白中的應用。Study on serum - free medium for 5b1 cell growth
1細胞生長的無血清培養基的研究A successful cell strain for biopharmaceutical production must conform to the following characteristics : the cells could produce high level of recombinant proteins ; the cell should be adapted to serum - free or protein - free medium ; the cells should be resistant to adverse culture conditions, which means the cells should have some anti - apoptosis property ; if not cultured i n suspension, the cells should also be able to grow in adherence
一株成功的工程細胞除了要求目的蛋白的表達量高外，還必須適應無血清培養基培養；必須具有對不利環境的抵抗能力，即抗凋亡能力；對于非直接懸浮培養的細胞，還必須具備在無血清培養條件下的貼壁能力。分享友人