血清中和試驗 的英文怎麼說
中文拼音 [xiěqīngzhōnghéshìyàn]
血清中和試驗
英文
protection test- 血 : 血名詞(血液 多用於口語) blood:吐血 spit (up) blood; 血的教訓 a lesson paid for [written] in b...
- 清 : Ⅰ形容詞1 (純凈) unmixed; clear 2 (寂靜) quiet 3 (清楚) distinct; clarified 4 (一點不留) w...
- 和 : 和動詞(在粉狀物中加液體攪拌或揉弄使有黏性) mix (powder) with water, etc. : 和點兒灰泥 prepare some plaster
- 試 : 名詞(古代占卜用的器具) astrolabe
- 驗 : 動詞1. (察看; 查考) examine; check; test 2. (產生預期的效果) prove effective; produce the expected result
- 血清 : [免疫學] serum; blood serum血清病 serum sickness
- 試驗 : trial; experiment; test
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The serum samples of foxes were gathered after the last vaccinated. the liters of the neutralization antibody against cdv and hemagglutination inhibitation antibody against cpv, cav were detected
分別用微量中和試驗測定血清中cdv的中和抗體效價,用血凝抑制試驗測定血清中抗cav 、 cpv的hi抗體效價。4 termer m. humoral immune response in human tuberculosis : immunoglublines g, a and m directed against the purified 12 protein antigen of mycobacterial bovis bacillus calmette g verin. j c lin microbiol, 1988, 27 : 1714
3黃蓉蓉.酶聯免疫吸附試驗檢測肺結核病人血清抗體的初步研究.中華結核和呼吸雜志, 1983 , 6 : 351Micro - serum neutralization test for swine vesicular disease virus
豬水庖病病毒微量血清中和試驗Micro - serum neutralization test, virus isolation and identification for bluetongue
藍舌病微量血清中和試驗及病毒分離和鑒定方法In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。The neutralization test is based on the neutralization of virus by specific antibodies found in the serum.
中和試驗是基於病毒可被存在於血清中的特異性抗體。At the same time, 10 serum sample were detected by elisa kit made in abroad and agp. the result of three elisa detection shows : s / p of 8 detected serum higher than 0. 2 is positive ; s / p of 2 detected serum is negative. the result accords with the result of agp detection
最後,再將自製vp1板用自配試劑檢測其活性,同時用自製板和國外試劑、國外elisa試劑盒、瓊脂擴散試驗檢測同樣10份血清作比較,三組elisa檢測結果,其中有8份被檢血清s p (抗體水平)大於0 . 2 ,為陽性; 2份被檢血清為陰性。Serum neutralization test for duck virus hepatitis type virus
鴨病毒性肝炎型病毒血清中和試驗Animal health analysis method - detection of specific antibodies of mycoplasma gallisepticum, mycoplasma meleagridis or mycoplasma synoviae in serum by the rapid serum agglutination test
動物健康分析方法.用快速血清凝集試驗檢測血清中支原體gallisepticum支原體meleagridis和支原體滑液的特定抗體24, 48, 72 hours later after transfection, we testified the expression of pp38 with mab h19, and pp24 with the antiserurn of pp24 expressed in e. coli. the tests justified the expression of pp24 in prokaryotic and eukaryotic expressing systems. in order to study the correlation of pp38 and pp24, we cloned pp38 gene and pp24 gene into pbudce4
為研究pp38和pp24之間的關系,將型mdvmd11株的pp38基因和pp24基因的完整orf克隆到真核雙表達載體pbudce4 . 1中,轉染cef后,通過ifa檢測和用抗pp24多克隆血清進行western - blotting試驗檢測到了pp38和pp24磷蛋白的共表達。Spf chicken - serum neutralization test
Spf雞血清中和試驗Detection antibodies against foot and mouth disease virus - protocol of micro - serum neutralization test
口蹄疫病毒抗體檢測方法.微量血清中和試驗Medium which is serum - free or in the low concentration serum inhibit the proliferation of cells. a certain degree of increase serum concentration can promote the growth rate as well as prolong the life. the feeder layer cells were provided by mef, mue ( mouse uterus epithelium ) and rmc ( rat myocardial cells )
Mef和es細胞體外培養血清濃度以15 20為宜,試驗中以mef 、 mue 、 rmc作飼養層,結果表明:以12 . 5dpc的mef為飼養層,無論是在小鼠es細胞的原代培養,還是在克隆傳代過程中,效果都是最佳的。Sds - page and westen - blotting validated the expression of pp24. the expressed specific band was excised from the gel and injected into mice once two weeks for 5 times, then we collected the antiserum from the mice and used it for ifa with chicken embryonic fibroblasts ( cef ) infected by mdv mdll, cvi988 and ga strains respectively. the positive straining was found in the mdv plaques, which shows the in vitro expressed protein of pp24 has some epitopes of mdv
將型mdvmd11株的pp38基因和pp24基因的完整orf分別克隆入真核表達載體pcdna3 . 1 / zeo ( + )中,重組質粒pcdna3 . 1 - pp38和pcdna3 . 1 - pp24在脂質體作用下分別轉染cef ,在轉染后24 、 48 、 72小時,用單抗h19和pgex - pp24原核表達產物制備的抗血清分別對pcdna3 . 1 - pp38和pcdna3 . 1 - pp24轉染的cef進行ifa檢測,結果檢測到了pp38和pp24在cef中的表達,該試驗也使pp24基因在原核和真核系統中的表達得到了相互驗證。分享友人