血清比 的英文怎麼說
中文拼音 [xiěqīngbǐ]
血清比
英文
serum ratio-
Compared with the known antibodies, the antiserum we prepared is highly specific and rules out the possibility of cross - reacting with angiotensin fragments. 4
與己有抗體相比，本實驗制備的抗agt多克隆抗血清不僅具有較高的特異性，而且排除了與血管緊張素短片段的交叉反應。The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。Feasibility observation of 28 biochemical ssay index between anticoagulant blood plasma with heparin lithium and blood serum
肝素鋰抗凝血漿與血清樣品28項生化檢驗項目的可比性分析It is found that this qcm technique is not only to be comparable to the surface plasmon resonance ( spr ) in response characteristics, but also more simple, cheap and reproducible in its equipments. the fourth part, an amplified by peg mass piezoelectric immunoassay has been developed to detect the ceruloplasmin in human serum
將之用於測定了人血清白蛋白（ hsa ）抗體的活性，並與文獻報道的表面等離子體子共振法（ spr ）進行了比較，發現該傳感系統的響應性能可與spr法媲美，而且較之具有儀器簡單廉價、再生簡便等優點。Experiment shows that the antigen extracted by autoclave is best for dot - ppa - elisa. applying the x2 test, optimal concentration of antigen was determined, and the optimum dilution of enzyme hrp - spa was 1 : 10. the high specificity of dot - ppa - elisa was proved by the specific blocking test, and also by the cross - reaction test in which the diaphragm does not react with the antibodies against salmonellosis, pasteurellosis, chlamydiosis, hcv, ppv, brucellosis, erysipelas suis, colibacillosis, prv
試驗中對多種方法提取的診斷抗原進行了比較、篩選；確定了dot - ppa - elisa的最佳工作條件；測定了st - 171株的免疫豬血清抗體及c群、 2型人工感染豬血清抗體效價，並初步確定了該方法的陽性標準。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。In a comparison of nephron - sparing surgery and radical nephrectomies, the tumor size, operative time, blood loss, hospital stay, and preoperative and postoperative serum creatinine levels were analyzed
比較腎臟保留手術和根除性腎臟切除手術的差異，我們針對腫瘤大小、手術時間、血液流失程度、住院日數、術前和術后的血清肌酸酐值均加以分析及討論。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again
應用間接免疫熒光試驗進行血清學鑒定，結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序，在genbank上進行同源性比較，發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高，分別為94 、 95 ，從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上，本實驗對兩株病毒進行了核苷酸全序列測定。Guidelines for the immuno - turbidimetric determination of serum apolipoprotein a 1 and apolipoprotein b
血清載脂蛋白a1及載脂蛋白b免疫透射比濁測定法The endocrine cells in the digestive and glands of alligator sinensis embryos aged from 8th to 55th day were localized and compared by using immunohistochemical method with thirteen kinds of antiseras of hormone. during the development of pancreas in alligator sinensis embryos, somatostatin ( ss ) immunoreactive ( ir ) cells, 5 - hydroxytryptamine ( 5 - ht ) - ir cells, glucagon ( glu ) - ir cells, epidermal growth factor ( egf ) - ir cells appeared on 18th day. no p53 protein - ir cell, gastrin - ir cell, testosterone - ir cell, chromogranin a - ir cell, vasoactive intestinal polypeptide - ir cell, epithelial membrane antigen - ir cell or insulin - ir cell was found in the pancreas of alligator sinensis embryos
本實驗採用免疫組織化學技術，應用13種不同的抗血清，對孵育時間8 ? 55天揚子鱷胚胎消化道及消化腺內分泌細胞的種類進行鑒別、定位和比較，結果如下：揚子鱷胚胎胰腺中，生長抑素、 5 ?羥色胺、胰高血糖素、表皮生長因子、胰多肽免疫反應陽性細胞出現于第8天； p物質免疫陽性細胞出現于第18天； p53 、胃泌素、睪酮、嗜鉻素a 、血管活性腸肽、上皮膜骯原、胰島素免疫陽性細胞在各期揚子鱷胚胎胰腺中均未發現。The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production
應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。Comparison of serum trace element level between the coronary heart disease patients and the health volunteers
冠心病患者與健康人血清微量元素水平的比較Tentative method for quantitative analysis by immunoturbidimetry of human serum albumin
人體血清清蛋白免疫比濁法定量分析的暫行方法The purposes of the present study were to find out the changes regularity and provide some theoretical basis and guidance to the training, competition and recovery of the athletes. these problems have not been studied nowdays. it has not been reported for the effects of different exercises on kidney function of wushu routine athletes
以尿蛋白、白蛋白、 _ 2 -微球蛋白作為反映運動員腎功能狀態的指標，分析了血管緊張素對運動員腎功能的影響，試圖找出不同形式訓練對運動員腎功能影響的變化規律及其產生的可能機制；同時測定了血清肌酸激酶和血尿素氨來觀察運動員的身體機能狀態和恢復情況，為運動員進行科學訓練、比賽和恢復提供一些實驗依據。To explore the effect of bamboo stick contacting time with serum sample on serum potassium ion concentration, we have systematically measured serum potassium ion concentration of bamboo stick contacting with serum sample at different times using ion selective electrode method and analyzed their potassium ion concentrations using matching t test
摘要為探討竹簽接觸血清樣品時間對鉀離子濃度影響的程度，採用離子選擇電極法分別對竹簽在血清中浸泡不同時間的樣品鉀離子濃度進行測定，並以配對t檢驗對不同浸泡時間的血清鉀離子濃度進行對比。Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province. 2. the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough
應用熔解曲線法和pcr微板核酸雜交- elisa法對44例接受拉米夫定治療過程中出現病毒學反跳時的血清進行ymdd突變株的檢測，並與測序法和mpcr - rflp法比較它們的敏感性、一致性。By morphologic examination, structure characteristics ^ biological property n serological tests and pathology characteristic study, this virus was identified as the medium virulence chicken. new castle disease virus ( ndv ), whose pathology characteristics were appeared different from those of the standard virulence ndv. la sola vaccine could not give good protection against the infection of this virus isolate
用spf雞胚在吉林省患病雞群中分離到一株病毒，經病毒形態學、結構特徵、生物學、血清學及致病性等試驗研究，確定該病毒為雞新城疫強毒，對lasota免疫雞的致病性及其致死率與雞新城疫標準強毒e _ 9f _ （ 48 ）對比差異顯著， lasota疫苗對該株病毒免疫保護效果不理想。S, . antibodies were monitored at interal of one week, the results showed that it is positive between 2 to 4 weeks after inoculation ; the mice were weighed every 5 day, the results showed that weight difference is significant between the inoculated and control group. seroreaction of test group is positive using hbsag elisa kit, but control group is negative
6 、動物試驗：將重組矗因工程苗苗誘導表達波免疫家兔，然後每周采血分離血清測邑鉆抗體，結果在免疫后2周，血渝中檢出ss抗體；兔疫注射10日齡小白鼠，每5天稱重一次，結果表明：注射誘導表達液的試驗組比對照組槽重差異顯著叩0New york ( reuters health ) jun 20 - the proportion of plasma cells in bone marrow and the serum monoclonal protein leel at diagnosis influence the risk that smoldering ( asymptomatic ) multiple myeloma will progress to symptomatic disease, new research suggests
紐約路透通訊社健康欄6月20日訊：最新研究發現，骨髓血漿細胞和血清單克隆蛋白水平的比例會影響無癥狀性多發性骨髓瘤發展為有癥狀性骨髓瘤的風險。分享友人