衣藻型的 的英文怎麼說
中文拼音 [yīzǎoxíngde]
衣藻型的
英文
chlamydomonad-
In the third part of the thesis, a chlamydomonas reinhardtii chloroplast expression vector, pactbvpl, containing the fusion of the foot and mouth disease virus ( fmdv ) vp1 gene and the cholera toxin b subunit ( ctb ) gene was constructed. transformation of c. reinhardtii chloroplast was achieved by biolistic bombardment with pactbvpl
論文第三部分主要敘述了將o型fmdvvp1與強黏膜免疫佐劑霍亂毒素b亞基（ ctb ）的融合基因克隆重組到衣藻葉綠體表達載體中，並採用基因槍法轉化衣藻葉綠體，獲得了具有壯觀黴素抗性的轉化子。To determine that the integration of foreign genes had occurred hi the chloroplast genome at the directed site by homologous recombiantion and to evaluate the degree of homogeneity of the c. reinhardtii transformants, pcr and pcr - southern blot analysis were performed using the primers of c. reinhardtii chloroplast homologous fragment
並根據衣藻葉綠體同源片段chll基因，設計一對引物對其葉綠體dna進行的pcr和southernblot分析。結果表明外源基因已經定點整合入衣藻葉綠體基因組的chll基因中，並且經過三輪抗生素篩選所得的衣藻葉綠體轉化子中，轉基因型葉綠體基因組拷貝佔多數。
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