表異構物 的英文怎麼說
中文拼音 [biǎoyìgòuwù]
表異構物
英文
epimeride- 表 : Ⅰ名詞1 (外面;外表) outside; surface; external 2 (中表親戚) the relationship between the child...
- 異 : 形容詞1 (有分別; 不相同) different 2 (奇異; 特別) strange; unusual; extraordinary 3 (另外的;...
- 構 : Ⅰ動詞1 (構造; 組合) construct; form; compose 2 (結成) fabricate; make up 3 (建造; 架屋) bui...
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
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Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板,用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增,得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上,篩選反向克隆,然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游,構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上,通過花粉管通道法轉化河套蜜瓜,共獲76顆瓜,並進行了硬度和含糖量的分析。In this thesis, the development of emulsion polymerization theory and related new technologies are comprehensively reviewed, and the synthetic technologies of acrylate latex modified by organosilicon both at home and abroad are also reviewed. based on the current development and research in this field, attentions of this study are focused on three ways for preparing novel acrylate latexes modified by organosilicons, that is, 1 ) the acrylate monomers are copolymerized with unsaturated silicons by emulsion copolymerization ; 2 ) a new latex with microphase separation morphology is synthesized, which shows a core - shell structure character ; 3 ) soap - free polymerization is explored for preparing a higher - performance latex using an unsaturated silicon monomer, different acrylates and a reactive emulsifier. by using techniques of delaying addition of organosilicon monomer and a hydrolysis inhibitor, the hydrolysis and condensation of 3 - ( trimethoxysilyl ) propyl methacrylate during polymerization can be effectively prevented, as a result, organosilicon content in the macromolecular chain is increased
同時採用有機硅單體延遲滴加及添加水解抑制劑等技術,有效防止了-甲基丙烯酰氧基丙基三甲氧基硅烷在乳液聚合過程中的過渡水解及縮合反應,提高了聚合物大分子鏈中有機硅鏈節的含量;利用紅外光譜與差示掃描量熱儀對產物分子結構進行了表徵,並通過對共聚產物力學和吸水率的測試,證實了本研究所制備的有機硅改性丙烯酸酯共聚物具有比純丙烯酸酯聚合物更優良的力學及耐水性能;從分子設計角度出發,利用醇解反應合成出水解、縮合反應速率較慢的兩種新型不飽和有機硅單體: ?甲基丙烯酰氧基丙基三乙氧基硅烷和?甲基丙烯酰氧基丙基三異丙氧基硅烷;利用核磁共振對新型硅烷單體結構進行了證實;研究表明利用新型硅烷單體可以制備出高硅烷含量的有機硅改性丙烯酸酯乳液,且其聚合產物具有較高的力學及耐水性能。Gene engineering technology is more superior than the cross breeding and directive breeding technology with its short cycle, low cost and high benefit. though traditional breeding technology has been used for a long time. now the direct reports for the changes of the flower color by the chi ( chalcone isomerase ) gene are a few what we known.
關于花色結構基因查爾酮異構酶chi ( chalconeisomerase )基因對花色改變的直接報道很少,因此,本論文選用了chi基因為目的基因,以純深紅色和純白色矮牽牛( petuniahybidavilm . )為材料,研究了chi基因的共抑制和反義抑制以及表達產物增加對花色改良的作用,並在花色改變植株中首次觀察到花器官變異。As an important part of the morph - functional research of animals, the diversity of hair micro - structure is of significance for the discovery of species adaptation and evolution. hair structural diversity provides parameters to interpret the divergent adaptation and evolution tendency in different ecocommunities. physical principles introduced probed into the interpretation of the mechanism that the hair microstructure forms and the relatedness between structure and function, animal behavior and ecology
毛發微觀結構多樣性研究作為動物形態與功能多樣性研究的內容之一,對揭示毛的形態結構與功能多樣性在物種生存和適應中的意義,具有積極作用;同時作為生態形態學的研究內容,對解釋不同生態類群中的動物體形態結構的適應和進化趨勢以及所表現出的趨同、趨異現象也將發揮重要作用;在結構形態學研究方面,應用物理學原理解釋毛的微觀結構形成的過程以及結構與功能、行為、生態的關系也很重要。Many studies show that leafy is high homolog even among distantly related plant species. exception of these, little studies on tissue culture and transformation of ginkgo have been done. this paper emphasizes on the isolation, cloning and analysing two ginkgo orthologs of leafy from the male tree
為此,本實驗從銀杏leafy同源基因的克隆入手,分析其雌雄株lfy基因結構差異,構建lfy基因的植物正義反義表達載體,建立矮牽牛遺傳轉化體系,以研究銀杏lfy同源基因的功能,同時建立了銀杏組織培養體系,為銀杏的遺傳轉化和提早開花結果奠定基礎。The results show that ( 1 ) loops are in general more variable than stems, and in loops a strong adenine bias are observed : ( 2 ) there does not exist a saturation effect in stems, loops or all positions of the 16s rrna gene fragments : ( 3 ) in the molecular cladogram, bagarius forms a sister group with glyptothorax, and euchiloganis forms a sister group with pareuchiloglanis, and exostoma forms a sister group with glyptosternum : ( 4 ) the phlogenetic positions of pseudecheneis, exostoma and glyptosternum are not recognized ; the glyptosternoid fishes are not monophyly although they are defined by 13 osteological apomorphies ; the incongruence of cladograms between molecular and morphological sets may be caused by less informative sites of the 550 homologous sites ; ( 5 ) e. davidi and e. kishinouyei could be the same species according to the genetic distances ; p. sinensis and p. anteanalis could be too
結果表明: ( 1 )環區平均變異位點較莖區多,有很強的a偏好性; ( 2 )沒有替代飽和現象; ( 3 )分子系統樹上?屬和紋胸?屬構成姐妹群,石爬?屬和?屬構成姐妹群, ?屬和原?屬構成姐妹群; ( 4 ) ?屬、原?屬和褶?屬的系統發育位置不定, ? ?魚類並未形成一個單系類群;可能的原因是所得到的16srrna基因片段信息位點太少; ( 5 )青石爬?和黃石爬?可能是同一物種,中華?和前臀?可能是同一物種。Through the research on the development character of overthrust fault, we analyzed its sealing mechanism. we also established evaluation standard and method of fault sealing ability through the following two simulation experiment : experiment on the quantitative relationship between property of fracture zone filler and fault sealing ability ; experiment on the deformation character of cream shale. based on these work, we evaluated sealing ability of overthrust faults on several typical structure in kuche depression
本文通過逆掩斷層發育特徵研究,分析了逆掩斷層封閉機理,在斷裂帶填充物性質與斷層封閉性定量關系模擬實驗和膏泥巖變形特徵模擬實驗基礎上建立了斷層封閉性評價標準及評價方法,以此對庫車坳陷幾個典型構造上逆掩斷層垂向封閉性進行了評價,結果表明現今斷層多是封閉的,這是庫車塌陷天然氣和異常孔隙流體壓力得以保存的主要條件。Their differences are based on many aspects which include characteristics of morphology, anatomy and epidermis of leaf, types of tapetum in anther walls, patterns of endothecial thickenings, and ways of development of endosperms, presence or absence of perisperm, components of photochemistry, and sequences of rbcl
菖蒲屬與天南星科其它屬在葉的形態、結構、表面特徵,花藥絨氈層類型,藥室內壁增厚的特點,胚乳的發育方式,外胚乳的有無,植物化學成分, rbcl基因序列等多方面存在著顯著的差異。In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上,根據國內外已發表的ibv基因序列,分別設計特異性引物,應用不同引物進行反轉錄合成cdna ,分片段對ibv的主要結構基因進行pcr擴增,並分別將各個目的片段克隆到puc19載體上,在大腸桿菌dh5中實現目的基因的分子克隆,經藍白斑篩選、限制性內切酶分析、 pcr鑒定,篩選出重組陽性質粒,並對各個目的基因片段進行序列測定,從而獲得ibv主要結構基因全序列。Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch
利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸,須在宿主細胞引入異源酶基因擴展代謝途徑;串聯表達酶基因,同時適量增加不同種屬的多個關鍵酶酶量,改善限速反應;利用同源重組進行基因整合和基因破壞,改造染色體結構定向改變微生物代謝途徑;目的是將碳代謝流最大程度的引向奎尼酸生成的方向。Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively
用bamhi和saci同時酶切ps2 ( s2表示來自雷司令的芪合酶基因) 、 ps1 ( s1表示來自粉紅玫瑰的芪合酶基因)以及pbi121 、 pev2 ,使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因,構建成植物表達載體pbs2 、 pev2s1 , pbs2中含camv35s組成型啟動子,使s2基因能在番茄植株的各個部位表達; pev2s1則含有果實特異性啟動子tfp2 ,使s1基因只在番茄果實中表達。The species that make up natural ecosystems usually display a wide range of genetic variability.
構成自然生態系統的生物種,通常表現有較大范圍的遺傳變異性。Study on branchlet surface sculpture does not reveal any hair - like structures as recorded by previous authors ; only some warty cuticles on the ridges and variable stomata in the grooves are noted. the surface of synangium in ephedra has secondary sculpture while the lip of the openings of synangium resembles the cerebral waves
對小枝表面掃描電鏡下的微形態構造的研究並沒有發現前人報道的毛狀體特徵,而只有一些疣狀角質突起和有變異的氣孔,同時注意到,麻黃屬植物的小枝表面特徵受到生境、發育階段的影響。It may have a preventional function from alzheimer ' s disease. molecular biological studies are shown that apoe isoform has a isoform - specific role to neurodegeneration. apoe3 and apoei aid to sustain the stabilization of cytoskeleton protein tau, while apoe4hyperphosphorylates tau to destabilize the microtubule protein, which will make neurocyte easy to form neurofibrillary tangles in ad
分子生物學研究發現, apoe異構體對神經細胞退化表現出異構體特異作用, apoe _ 3和apoe _ 2有助於維持微管的穩定性,而apoe _ 4則使tau蛋白超磷酸化,破壞微管的穩定性,使神經細胞易於形成神經纖維纏結。Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 ( hpf4 )原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點,設計合成一對特異引物,在pt7 - 7 - rpf4表達質粒的基礎上,應用聚合酶鏈式反應( pcr )對其cdna進行改造,通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ,用快速pcr檢測法、 dna測序分析,鑒定重組hpf4表達質粒的正確性。In the laboratory experiment part, human peripheral blood, cultured cells and icr mice were study objects. the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test. the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test. the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry, western blot and rt - pcr
流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關,為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制,本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象,用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響,用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage
構建了echistatin融合蛋白表達載體,經dna測序鑒定正確后,溫控誘導表達,獲得了高效表達,表達產物經505一隊ge分析,分子量為16000oa ,符合預計的融合蛋白分子量,表達產物. ll 』菌體總蛋白的30 %以上, westem一blotting結果顯示,該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應,表明我們成功構建了eohistatin的高效融合表達克隆。At the same time, the mechanics mode of newly - built structure is not the same as that single cavern is excavated in the half - unbounded space. its original stress field is disturbed by former time and time again, and once more by excavating of later. so the mechanics mode of the later is not symmetrical generally and infinite variability is put up
同時新建結構物的受力模式也不同於半無限體或無限體中修建單一洞室的一般狀況,其初始應力場往往是經過多次擾動的,其施工將再次進行擾動,使其受力往往是非對稱的,表現出極大的變異性。Image difference is the most direct change detection method for change area extraction, but the gray difference image that is only based on spectral feature is difficult in describing the structure change of an objects
圖像差分是實現變化區域檢測的最直接方法,但是僅僅根據地物光譜特徵差異得到的灰度差分圖像難以表徵地物局部結構的變化。Ingl plasndd was a1so prepared and delivered to the npc cell. subsequenly, v mbl was also de1ivered to the cell and intensity fluorescence was observed by nuorescence confocal ndcroscope. the degree of fluorscence enhancemen can be wttatively obtaind through anscence spectr8
4這種探針對乙肝病毒陽性和陰性病人血清的檢測有顯著性差異,生物素化的分子信標可以用橋式結構固定在玻璃片表面,製成dna晶元,根據晶元的熒光可以快速檢測到靶g dna的存在。分享友人