親合標記法 的英文怎麼說
中文拼音 [qīngěbiāojìfǎ]
親合標記法
英文
affinity labeling- 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
- 標 : Ⅰ名詞1 [書面語] （樹梢） treetop; the tip of a tree2 （枝節或表面） symptom; outside appearance; ...
- 記 : Ⅰ動詞1 （把印象保持在腦子里） remember; bear in mind; commit to memory 2 （記錄; 記載;登記） writ...
- 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
- 標記 : （標志; 記號; 貨物標記） tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
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In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。
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