親和洗脫 的英文怎麼說
中文拼音 [qīnhéxǐtuō]
親和洗脫
英文
affinity elution- 和 : 和動詞(在粉狀物中加液體攪拌或揉弄使有黏性) mix (powder) with water, etc. : 和點兒灰泥 prepare some plaster
- 洗 : 洗動詞1 (用水等去掉物體上的臟東西) wash; bathe 2 [宗教] (洗禮) baptize 3 (洗雪) redress; ri...
- 脫 : Ⅰ動詞1 (脫落) cast; shed; drop; fall off 2 (取下; 除去) take off; strip; cast off 3 (脫離) ...
- 親和 : affine親和嫁接 congenial graft; 親和數 amicable number; friendly number
- 洗脫 : eluation
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The recombinant protein exhibited specific binding to antiviral antibodies of h5 subtype and has no cross - reaction with h7 and h9 subtype
分別用親和層析以及電洗脫兩種方法對gst - ha1融合蛋白進行了純化,都獲得了理想的純化結果。The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively
方法是先用硫酸綏鹽析,再過親和層析柱,即33飽和硫酸按沉澱后,透祈過夜,上p24抗原做配體的濱化氰活化的sepharose4b柱,流洗掉雜蛋白和抗人紅細胞單特異性抗體后,再洗脫並收集雙特異性抗體。Purification was conducted with ni - nta resin as there ' s a 6 x his tag at the n - terminal of the recombinant protein. after stepwise washing and elution at purification procedure, sublimated protein was obtained with the full - length ns5 accounting for more than 90 % and the aborted translated protein about 6. 5 %
用ni一nta樹脂親和純化表達產物,經過梯度漂洗和洗脫,獲得純度90 %以上的全長表達產物,另有6 . 5 %的非全長翻譯產物(約70kda ) 。
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