解凍期 的英文怎麼說
中文拼音 [jiědòngqī]
解凍期
英文
break－up period-
The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52
以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency
多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。This paper unfolds engineering disposition and theoretical research of negative temperature high performance concrete ( nthpc ) with the damage of many factors motivation, the project background based on qing - zang railway engineering, which is one of four - emphasized engineering during the tenth five - plan. due to the difference of environment of construction and service between nthpc and ordinary concrete, and therefore durability of nthpc is required to higher level. at a first, nthpc must avoid frozen damage at early age and possess anti - freezing property at later period, in order to meet engineering practicable application need, and this are two emphasized and difficult problems, as for nthpc ; by means of mechanism analysis about deicing - agent ingredient and anti - freezing at early period, adopting composite technique routine of mineral addition + anti - freezing element + water - reducer4 - air - entraining + anti - erosion of steel component etc, based on orthogonal experimental approach, fd - 1 composite functional admixtu re was manufactured, which has more property and orientation on qing - zang railway
負溫混凝土由於和普通混凝土在施工環境及服役環境上存在的差異，因此表現為比普通混凝土更為較高的耐久性要求；負溫混凝土首先要避免早期的凍害以及具備長期抗凍性能，才能夠滿足工程實際應用的要求，這也是負溫混凝土必須解決的兩大技術關鍵；通過對目前常用防凍劑組分作用機理的分析研究及混凝土早期防凍機理探討，採用礦物外加劑+防凍組分+高效減水劑+引氣+阻銹組分功能復合的技術路線，通過正交試驗設計復配了適應青藏鐵路工程要求的專用多功能復合型外加劑fd - 1 ；並在此基礎上配製不同等級負溫高性能混凝土，開展一系列包括硫酸鹽侵蝕、氯離子滲透、抗凍融循環、收縮及耐磨性等耐久性能研究；通過對fd - 1組分和摻量的調整，優化負溫混凝土在施工特性、力學指標和耐久性三個方面的兼容、協調性。Abstract : by using early - strength frost - resistant agent in consolidatinganchor bolt, extra early - strength frost - resistant thickener and special technology in shotcreting, the difficulty arising from freezing weather with an ambient temperature of - 10 - 18 was solved
文摘:介紹在環境氣溫- 10 - 18條件下，採用早強抗凍錨固劑固結錨桿、超早強抗凍粘稠劑和特殊工藝噴射混凝土，使隧道施工支護及時，解決了冬期施工噴錨難的老大難問題。Calculation of the loss of runoff for the frozen ground during melting period ; 7
凍土融解期產流損失的計算； 7流域蒸散發的時變性。Tvb - n value, tba value, dehydration rate, thawing waste, cook drip were measured after being stored a period of time
定期取樣測定其揮發性鹽基氮（ tvb - n ）值、硫代巴比妥酸值（ tba ） 、冷藏乾耗率、物料解凍時的汁液流失量及煮汁率。That, at least, is what reiko yoshida, head of a small food firm in western japan, told a news conference when asked to explain why her company sold frozen spring rolls and other products that were well past their sell - by date1
至少，日本西部一個小食品公司的主管吉田玲子音在記者招待會上是這么說的，當時人們要求她解釋為何她的公司要出售已過期很久的凍春卷和其他產品。2. 32 discarded unmutured oocytes on dayo, forty - two discarded unfertilized oocytes on day2 and 91 discarded unfertilized oocytes on day3 ( dayo refers to the day of oocyte collection ) from 53 icsi cycles ; 127 normal fertilized embryos from 62 ivf and 41 icsi cycles with a grade of ih - ivand improper to freeze after day3 embryo transfer ; 97 discarded embryos after day3 thawed - embryo transfer with a grade of iii - ivfrom 39 cycles. 3
天）廢棄的未成熟卵子32個，受精后第二天山2天）未受精卵子42個和第三天口3天）未受精卵子91個; 62個ivt和41個icsl周期中d3天移植后評分較低的in一w級不適宜冷凍的正常受精胚胎共127個， 39個解凍周期中移植后廢棄的m一w級胚胎97個。Have to hand in, do not hand in the most serious consequence to close down namely withhold inn inside as equivalent as duty goods, or undertake freezing to your bank deposit account, pay tax till you till, if be not handed in namely inside proper time, can come to goods auction so pay imposition, or be deduct tax payment directly from inside bank account, morning and evening you should be handed in, be inferior to delighted hand over item by item, also have profit to you so, if duty wu asks the problem cannot be solved, so you cannot do the processing that abandon line of business in industrial and commercial bureau, is the trouble bigger
不能不交，不交最嚴重的後果就是查封扣繳店內與稅等值的貨物，或對你們的銀行存款賬戶進行凍結，直到你們交稅為止，假如在一定期限內就是不交，那麼可以將貨物拍賣來繳納稅款，或是從銀行賬戶中直接扣除稅款，早晚你是要交的，不如愉快點交，這樣對你們也有好處，假如稅務問題解決不了的話，那麼你就不能在工商局做廢業處理，麻煩不是更大？Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。Many places in our country have long cold season, because of construction period limit, concrete construction in winter is not avoided, knowing concrete construction cold principle in winter is important, so relevant winter concrete construction method must be selected according to local conditions
摘要因為冬季施工有較長的寒冷季節，由於工期的制約，冬季進行混凝土施工不可避免，故而了解混凝土施工的冬季凍害原理並根據當地的具體條件選擇適當的冬季施工方案就尤為重要。In one of the radio phone - in programmes, a caller said the budget proposal he appreciated most was the abolition of estate duty because it would help resolve cash flow problems encountered by small businesses when their assets were frozen during the estate duty assessment period
一位聽眾告訴我，預算案中他最欣賞的就是取消遺產稅的建議，因為這可協助小本經營的市民解?因資產在評核遺產稅期間被凍結，以致資金周轉不靈的困難。分享友人