解凍液 的英文怎麼說
中文拼音 [jiědòngyè]
解凍液
英文
thawing solution-
The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52
以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。In the 2 - step ops method, embryos were pretreated with 10 % eg + 10 % dmso for 30s before exposed to edfs30, a vitrification solution, for 25s, then immersed in liquid nitrogen. the blastocyst rate of vitrified mouse morula after equilibration in 0. 5mol / l sucrose for 5min was 100 %
Ops二步法即胚胎預處理30s ，然後移入edfs30冷凍液中平衡25s冷凍保存，解凍后在0 . 5mol l蔗糖溶液中平衡5min ，培養后囊胚發育率為（ 100 ） 。Collection and preservation of samples : as soon as the three vital signs disappeared, the dogs were anatomized, and the heart, liver, kidney, spleen, lung, brain, muscle in the injection location and no injection location, the heart blood, urine, bile, cerebrospinal fluid ( csf ) in the lateral ventricle and spinal subarachnoid space, spinal cord ( medulla oblongata, cervical cord, the upper beast spinal cord, breast spinal cord and waist spinal cord ) were taken out, some of which were preserved at - 20 for qualitative and quantitative analysis, and the others were fixed with 4 % formaldehyde for the pathology observation
3 、樣品採集：當心電、血壓和呼吸全部消失時，迅速解剖動物，採取心臟、肝臟、腎臟、脾臟、肺臟、大腦、注射部位肌肉、注射部位20cm以外肌肉、心血、尿液、膽汁、側山西醫科大學碩士學位論文腦室腦脊液、脊髓腔腦脊液和不同節段的脊髓（包括延髓、頸髓、上胸部脊髓、胸部脊髓和腰部脊髓）等組織，冷凍保存。 4 、病理觀察:採取心臟、肝臟、 』腎臟、脾臟、肺臟、大腦、脊髓等組織， 4 %甲醛固定，石蠟包埋，切片， he染色，光鏡觀察。Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency
多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。Effects of thawing solution and oxytocin on the conception rates of cows inseminated with frozen semen
解凍液及添加催產素對母牛冷配效果的研究Characteristics : the freeze - dried vaccine looks like a pinkish crisp cake. after reconstitution it turns into a turbid liquid in pinkish color
：凍干疫苗為略帶粉紅色疏鬆體，溶解后為略帶粉紅色混濁液體。Tvb - n value, tba value, dehydration rate, thawing waste, cook drip were measured after being stored a period of time
定期取樣測定其揮發性鹽基氮（ tvb - n ）值、硫代巴比妥酸值（ tba ） 、冷藏乾耗率、物料解凍時的汁液流失量及煮汁率。After the acet is vaporized, the active substance in water is gotten. and which is vaporized at low temperature. then the crude active substance is purified by column chromatography on sephadex g - 75. after a series of purifications again, we could get some white powder at last. though the active substance is diluted to50 g / ml, the activity is still checkeded - up through phyto phtnora casicileon. the purified active substance is insensitive to heat, resistant to chloroform 、 ethanol and the orhers. in addition, the active substance is sensitive to high ph ( 10 ~ 14 ), but it is not sensitive to low ph ( 1 ~ 5 ). furthermre, when the ph is made to low again, the activity of it ' s comes back
用蒸餾水對菌體稀釋；加入適量吸附樹脂在150rpm 、 28下振蕩吸附4h ， 80 %的丙酮解吸，過濾解吸液得到活性物質的澄清溶液，旋轉蒸發儀旋轉蒸發去處丙酮，經sephadexg - 75分子篩層析得單一活性峰，收集峰值部分樣品液經冷凍乾燥得到淡褐色粉末，該活性物質用丙酮充分洗滌、甲醇-乙醚重結晶獲得略帶微黃的白色粉末，該活性物質50 g / ml仍可對蘇雲金芽孢桿菌hd - 1產生明顯的抑制作用。Flexor digitorum profundus tendons of chickens wer e cultured in the presence of deoxyguanosine ( dgua ) for 5 days, then cryopreserv ed in liquid nitrogen ( 196 ) without affecting their viability
用脫氧鳥苷培養處理雞屈趾深肌腱5天，無創凍存於液氮貯存器（ 196 ）中3個月，使用前將凍存腱在40溶液中融解並洗去腱中吸收的二甲基亞碸。Several fabrication methods, including nonwoven meshes / fiber bonding, solvent casting / particulate leaching, phase seperation / freeze drying, gas foaming and three - dimensional printing, which are used to prepare tissue engineered porous scaffolds from natural or synthetic biodegradable materials, are reviewed
摘要綜述了以天然或合成生物降解材料為原料的組織工程多孔支架的幾種制備方法，包括無紡織物纖維粘結法、溶液澆鑄粒子洗出法、相分離凍干法、氣體成孔法和三維「印刷」等方法。Transport and materials handling. road tankers for non - refrigerated liquefied and dissolved gas under pressure. floor filling and draining devices
運輸和裝卸.未冷凍壓力溶解液化氣道路罐車.下部裝卸裝置Bituminous mixtures - test methods for hot mix asphalt - part 41 : resistance to de - icing fluids
瀝青混合物.熱混瀝青的試驗方法.第41部分:耐解凍液Bituminous mixtures - test methods for hot mix asphalt - part 41 : resistance to de - icing fluids ; german version en 12697 - 41 : 2005
瀝青混合物.熱混合瀝青的試驗方法.第41部分:耐解凍液Cryomicroscope is an important tool in the research of low temperature biology and medicine. with the help of cryomicroscope, the dynamic, micron - scale, visual investigation of freezing and thawing processes in living tissues and individual cells can be carried out. in this paper, principles of low temperature biology and medicine are briefly introduced
人們可以藉助這一技術觀察到生物樣品在冷凍和復溫過程中的形態變化，以及溶液相變的過程，從而分析生物材料的相關特性，並以此為依據客觀的解釋實驗結果、修正理論模型和制定合理的治療或保存程序。Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。After washing with reagent ( 50mmol / l ph8. 0 tris - hcl, 100mmol / l nacl, 0. 5mmol / l edta, 2mol / l carbamide, 0. 2 % triton x - 100, 0. 2 % doc ), ultrasound crushing ( 200 w, 150 times, 3 seconds per time, spacing 3 seconds ) and freezing - melting methods, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %. 4. radioimmunoassay result shows that the radioimmune activity of of m - insulin fusion protein is 0. 5 unit per litre bacterial liquid
3 .表達產物初步純化仁gly 」一flumanin融合蛋白和人胰島素突變體融合蛋白包涵體的洗滌方案為: lj ]體沉澱溶於含zmol ， / l尿索洗滌液，超聲（ 200w 、巧o次、 3秒/次、間隔3秒） ， 4 、 i000orpm離心15min后沉澱用洗滌液充分重懸，一2 （ ）凍存過夜，次日融化后4 、 10000rpm離心15min ，取上清，即得到初步純化的融合蛋白，可去除土要的雜蛋白，融合蛋白溶解于上清液中，純度達到80 %以_ 1 : 。The eppendorf tube was immediately stored at - 80 c until the kinase assay was performed. the frozen eggs were frozen and thawed for three times, then added into 25l mpf buffer
Mpf及pka活性測定：將收集的鼠卵凍融3次，使細胞裂解，分別加人f反應液25山， 30t水浴反應7ruin 。分享友人